FlyBase Allele Report: Dmel\kto[T241]

General Information

Symbol

Dmel\kto^T241

Species

D. melanogaster

Name

FlyBase ID

FBal0121711

Feature type

allele

Associated gene

Dmel\kto

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Treisman, 2001)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q270term.

(Treisman, 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:19,837,544..19,837,544 [+]

Nucleotide change:

C19837544T

Amino acid change:

Q270term | kto-PA

Reported amino acid change:

Q270term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Treisman, 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Generation of kto^T241 clones specifically in the leg (using Scer\GAL4^Dll-em212 and Scer\FLP1^Scer\UAS.cUa) and in a Minute background results in severely stunted legs. In an other wild type background, these clones result in tarsal segmentation defects in all three paris of adult legs. Male prothoracic (T1) legs exhibit sex combs with slightly reduced teeth number but mostly aligned sex combs. Legs show strong distal leg shortening, and the most proximal tarsal joint as well as more distal joints are usually abnormal.

Generation of large kto^T241 clones specifically in the eye (using Scer\FLP1^ey.PB) in a Minute background results in the eye failing to differentiate ommatidia.

Heat-induced kto^T241 clones in the adult notum are associated with loss of bristles.

Generation of large Cdk8^K185 clones specifically in the eye (using Scer\GAL4^ap-md544 and Scer\FLP1^Scer\UAS.cUa) in a Minute background results in extensive loss of notal macrochaetes.

(Loncle et al., 2007)

kto^T241 clones in the anterior compartment of the wing disc which touch the anterior-posterior boundary often cross into the posterior compartment.

(Janody et al., 2003)

Clones in the eye contain few or no photoreceptors. The few that remain are found in posterior regions of the clone, and die later in development. Clone size is normal and clones do not interfere with the spread of differentiation-promoting signals. Antennal disc cell fates are also altered.

(Treisman, 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT suppressed by

Statement

Reference

kto^T241 has eye disc phenotype, non-suppressible by Delta::N^ΔECN.UAS/Scer\GAL4^ey.PH

(Treisman, 2001)

kto^T241 has eye disc phenotype, non-suppressible by ci^{m1-4.UAS.Tag:HA}/Scer\GAL4^ey.PH

(Treisman, 2001)

kto^T241 has photoreceptor neuron phenotype, non-suppressible by Delta::N^ΔECN.UAS/Scer\GAL4^ey.PH

(Treisman, 2001)

kto^T241 has photoreceptor neuron phenotype, non-suppressible by ci^{m1-4.UAS.Tag:HA}/Scer\GAL4^ey.PH

(Treisman, 2001)

kto^T241 has photoreceptor neuron phenotype, non-suppressible by hh^UAS.cAa/Scer\GAL4^ey.PH

(Treisman, 2001)

kto^T241 has eye disc phenotype, non-suppressible by hh^UAS.cAa/Scer\GAL4^ey.PH

(Treisman, 2001)

Other

Statement

Reference

Cdk8^K185, kto^T241 has sex comb tooth | somatic clone phenotype

(Loncle et al., 2007)

Cdk8^K185, kto^T241 has prothoracic tarsal segment | somatic clone phenotype

(Loncle et al., 2007)

Cdk8^K185, kto^T241 has eye | somatic clone - Minute background phenotype

(Loncle et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

The distal leg phenotypes provoked by Cdk8^K185, kto^T241 double mutant clones induced specifically in the leg (using Scer\GAL4^Dll-em212 and Scer\FLP1^Scer\UAS.cUa) are indistinguishable from kto^T241 alone but distinct from Cdk8^K185 alone, both for sex comb differentiation and for overall leg structure.

The phenotypes of Cdk8^K185, kto^T241 double mutant clones induced specifically in the eye (using Scer\FLP1^ey.PB) in a Minute background are indiscernible from kto^T241 alone.

(Loncle et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

kto^T555/kto^T241 is partially rescued by kto^UAS.cJa/Scer\GAL4^da.G32

(Janody et al., 2003)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

63126

w^*; kto^T241 P{neoFRT}80B/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Med12^T241

(Loncle et al., 2007)

kto^T241

(Kuang et al., 2020, de Ayala Alonso et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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