FlyBase Allele Report: Dmel\Ret[C168]

General Information

Symbol

Dmel\Ret^C168

Species

D. melanogaster

Name

FlyBase ID

FBal0216977

Feature type

allele

Associated gene

Dmel\Ret

Associated Insertion(s)

PBac{3HPy⁺}Ret^C168

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Soba et al., 2015)

Mutagen

Progenitor genotype

Associated Insertion(s)

PBac{3HPy⁺}Ret^C168

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:21,182,613..21,182,613 [-]

(Soba et al., 2015, FlyBase Curators, 2008-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Soba et al., 2015)

abnormal neuroanatomy (with Df(2L)BSC312)

(Soba et al., 2015)

Phenotype Manifest In

filamentous actin (with Df(2L)BSC312)

(Soba et al., 2015)

larval multidendritic class IV neuron

(Soba et al., 2015)

larval multidendritic class IV neuron (with Df(2L)BSC312)

(Soba et al., 2015)

Detailed Description

Statement

Reference

Homozygous Ret^C168 mutant larvae exhibit class IV dendritic arborizing (C4da) neuron dendrite patterning defects, resulting in out of plane crossing of isoneuronal branches.

More severe dendritic patterning defects are seen in Ret^C168/Df(2L)BSC312 mutant larvae. The C4da neurons do not show a significant reduction in dendrite length, but display excessive crossings of isoneuronal dendrites outside of their normal 2D growth plane and reduced dendritic field coverage. Large stretches of dendrites, in particular terminal arbors, are detached from the ECM and enclosed by the epidermis. These dendrite defects are seen from 48hr after egg laying. The C4da neurons also display disrupted dendritic dynamics compared to wild type, and aberrant F-actin localisation, with dendrites displaying increased proximal, but reduced distal, F-actin levels. Abnormal accumulation of F-Actin is seen in aberrant crossing Ret^C168/Df(2L)BSC312 mutant dendrites and many terminal dendrites exhibit lower levels of F-actin than controls.

(Soba et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Ret^C168/Df(2L)BSC312 has abnormal neuroanatomy phenotype, suppressible | partially by mew^UAS.cMBa/mys^UAS.cDa/Scer\GAL4^ppk.PH

(Soba et al., 2015)

Other

Statement

Reference

Ret^C168/Ret[+], mew^M6 has abnormal neuroanatomy phenotype

(Soba et al., 2015)

Ret^C168/Ret[+], mys¹ has abnormal neuroanatomy phenotype

(Soba et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

Ret^C168/Df(2L)BSC312 has larval multidendritic class IV neuron phenotype, suppressible | partially by mew^UAS.cMBa/mys^UAS.cDa/Scer\GAL4^ppk.PH

(Soba et al., 2015)

Other

Statement

Reference

Ret^C168/Ret[+], mew^M6 has larval multidendritic class IV neuron phenotype

(Soba et al., 2015)

Ret^C168/Ret[+], mys¹ has larval multidendritic class IV neuron phenotype

(Soba et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Ret^C168 mew^M6 transheterozygous third instar larvae exhibit defects in class IV dendritic arborizing neuron dendrite crossing and a substantial loss of dendrite-ECM interaction. No phenotypes are observed in either heterozygote alone.

Ret^C168 mys¹ transheterozygous third instar larvae exhibit defects in class IV dendritic arborizing neuron dendrite crossing and a substantial loss of dendrite-ECM interaction. No phenotypes are observed in either heterozygote alone.

Co-expression of mys^Scer\UAS.cDa and mew^{Scer\UAS.cMBa} in class IV dendritic arborizing neurons under the control of Scer\GAL4^ppk.PH partially suppresses the dendrite growth seen in Ret^C168/Df(2L)BSC312 mutant larvae and completely suppresses the loss of dendrite-ECM interaction. The reduction in dendritic field coverage is not suppressed.

Ret^C168 Rac1^J11 transheterozygous third instar larvae exhibit defects in class IV dendritic arborizing neuron dendrite-ECM interaction. The phenotype is not observed in either heterozygote alone.

(Soba et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Ret^C168/Df(2L)BSC312 is rescued by Ret^UAS.mCherry/Scer\GAL4^ppk.PH

(Soba et al., 2015)

Comments

Expression of Ret^{Scer\UAS.T:Disc\RFP-mCherry} in class IV dendritic arborizing neurons under the control of Scer\GAL4^ppk.PH rescues the dendrite growth and crossing phenotypes seen in Ret^C168/Df(2L)BSC312 mutant larvae.

(Soba et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Ret^C168

(Hoyer et al., 2018, Soba et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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