FlyBase Allele Report: Dmel\BEAF-32[AB-KO]

General Information

Symbol

Dmel\BEAF-32^AB-KO

Species

D. melanogaster

Name

FlyBase ID

FBal0215222

Feature type

allele

Associated gene

Dmel\BEAF-32

Associated Insertion(s)

Carried in Construct

Also Known As

BEAF^AB-KO

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Roy et al., 2007)

Mutagen

(Roy et al., 2007)

(Roy et al., 2007)

(Roy et al., 2007)

(Roy et al., 2007)

gene targeting by homologous recombination

(Roy et al., 2007)

Progenitor genotype

BEAF-32^A-KO

(Roy et al., 2007)

Cytology

Description

The duplication of BEAF-32 present in BEAF-32^A-KO has been reduced to a single copy which contains mutations that knock out both isoform A and isoform B function. One mutation eliminates the ATG codon of the BEAF-32A isoform and destroys an NsiI site. A second mutation eliminates the ATG codon of the BEAF-32B isoform and creates an ApaI site. A third mutation introduces two tandem stop codons into the exon shared by both A and B isoforms and destroys a BamHI site.

(Roy et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygous BEAF-32^AB-KO mutants are viable and do not have defects in pupal interommatidial cell number; these, BEAF-32^AB-KO/Df(2R)BSC429 animals and homozygous BEAF-32^AB-KO retinal clones (covering either the entire retina or a portion of the retina) have an increased proportion of pale R8 photoreceptors and a reciprocally decreased proportion of yellow R8 photoreceptors, compared to controls. Homozygous BEAF-32^AB-KO mutant clones (that cover a portion of the retina) do not have an altered ratio of pale to yellow R7 photoreceptors compared to control clones.

(Jukam et al., 2016)

Homozygotes cannot be maintained as a stock. They are weaker than their heterozygous siblings and die within a few days if they are not transferred to a new vial containing a limited number of flies. Homozygous males have normal fertility when crossed to wild-type females and can live at least 2 weeks if pampered.

Homozygous females have reduced fertility and the degree of fertility appears to be very sensitive to environmental conditions. Homozygous females mated to either homozygous or wild-type males lay fewer eggs than wild-type females, and the larval hatching rate of the progeny is approximately 40%. A higher proportion of progeny survive to pupal and adult stages if they are derived from the cross with wild-type (rather than homozygous) males, and the surviving adult progeny of this cross appear normal, are fertile and roughly equal numbers of female and male progeny are obtained.

Homozygous females (that have been mated with wild-type males for 4 days) have a normal number of ovarioles per ovary, but the mutant ovaries are smaller than wild-type, because most ovarioles lack mature oocytes. The mutant germaria appear normal. Oogenesis defects become apparent at around stage 8 or later. Some egg chambers have too many nurse cell nuclei (this could be due to the fusion of two egg chambers or to an extra round of cell division). Other egg chambers have small nuclei that are probably an intermediate step in egg chamber degeneration.

Homozygous flies have a rough eye phenotype.

The polytene X chromosome from the salivary glands of homozygous male larvae shows obvious structural defects; the banding pattern is lost and the chromosome appears shorter and puffier than normal.

(Roy et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

BEAF-32^AB-KO has female sterile | recessive phenotype, suppressible by Tofu¹/Tofu[+]

(McKowen and Hart, 2022)

BEAF-32^AB-KO has abnormal neuroanatomy | somatic clone | adult stage phenotype, suppressible | partially by sav^GMR.PT/wts^GMR.PT

(Jukam et al., 2016)

Df(2R)BSC429/BEAF-32^AB-KO has abnormal neuroanatomy | adult stage phenotype, suppressible | somatic clone by sd¹²/sd¹²

(Jukam et al., 2016)

BEAF-32^AB-KO has abnormal neuroanatomy | adult stage phenotype, suppressible | partially by ss^transgene6

(Jukam et al., 2016)

NOT suppressed by

Statement

Reference

BEAF-32^AB-KO has abnormal neuroanatomy | adult stage phenotype, non-suppressible by sev^unspecified

(Jukam et al., 2016)

Suppressor of

Statement

Reference

BEAF-32^AB-KO/BEAF-32^AB-KO is a suppressor of abnormal neuroanatomy | adult stage phenotype of ss^transgene6

(Jukam et al., 2016)

BEAF-32^AB-KO/BEAF-32^AB-KO is a suppressor of abnormal neuroanatomy | adult stage phenotype of sev^unspecified

(Jukam et al., 2016)

BEAF-32^AB-KO/BEAF-32^AB-KO is a suppressor | somatic clone of abnormal neuroanatomy | adult stage phenotype of sav^GMR.PT, wts^GMR.PT

(Jukam et al., 2016)

Other

Statement

Reference

Df(2R)BSC429/BEAF-32^AB-KO, sd¹² has abnormal neuroanatomy | somatic clone | adult stage phenotype

(Jukam et al., 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

BEAF-32^AB-KO has photoreceptor cell R8 of pale ommatidium phenotype, suppressible | partially by ss^transgene6

(Jukam et al., 2016)

BEAF-32^AB-KO has photoreceptor cell R8 of yellow ommatidium phenotype, suppressible | partially by ss^transgene6

(Jukam et al., 2016)

BEAF-32^AB-KO has photoreceptor cell R8 of pale ommatidium | somatic clone phenotype, suppressible | partially by sav^GMR.PT/wts^GMR.PT

(Jukam et al., 2016)

BEAF-32^AB-KO has photoreceptor cell R8 of yellow ommatidium | somatic clone phenotype, suppressible | partially by sav^GMR.PT/wts^GMR.PT

(Jukam et al., 2016)

Df(2R)BSC429/BEAF-32^AB-KO has photoreceptor cell R8 of pale ommatidium phenotype, suppressible | somatic clone by sd¹²/sd¹²

(Jukam et al., 2016)

Df(2R)BSC429/BEAF-32^AB-KO has photoreceptor cell R8 of yellow ommatidium phenotype, suppressible | somatic clone by sd¹²/sd¹²

(Jukam et al., 2016)

NOT suppressed by

Statement

Reference

BEAF-32^AB-KO has photoreceptor cell R8 of pale ommatidium phenotype, non-suppressible by sev^unspecified

(Jukam et al., 2016)

BEAF-32^AB-KO has photoreceptor cell R8 of yellow ommatidium phenotype, non-suppressible by sev^unspecified

(Jukam et al., 2016)

Suppressor of

Statement

Reference

BEAF-32^AB-KO/BEAF-32^AB-KO is a suppressor of photoreceptor cell R8 of pale ommatidium phenotype of ss^transgene6

(Jukam et al., 2016)

BEAF-32^AB-KO/BEAF-32^AB-KO is a suppressor of photoreceptor cell R8 of yellow ommatidium phenotype of ss^transgene6

(Jukam et al., 2016)

BEAF-32^AB-KO/BEAF-32^AB-KO is a suppressor of photoreceptor cell R8 of pale ommatidium phenotype of sev^unspecified

(Jukam et al., 2016)

BEAF-32^AB-KO/BEAF-32^AB-KO is a suppressor of photoreceptor cell R8 of yellow ommatidium phenotype of sev^unspecified

(Jukam et al., 2016)

BEAF-32^AB-KO/BEAF-32^AB-KO is a suppressor | somatic clone of photoreceptor cell R8 of pale ommatidium phenotype of sav^GMR.PT, wts^GMR.PT

(Jukam et al., 2016)

BEAF-32^AB-KO/BEAF-32^AB-KO is a suppressor | somatic clone of photoreceptor cell R8 of yellow ommatidium phenotype of sav^GMR.PT, wts^GMR.PT

(Jukam et al., 2016)

Other

Statement

Reference

Df(2R)BSC429/BEAF-32^AB-KO, sd¹² has photoreceptor cell R8 of pale ommatidium | somatic clone phenotype

(Jukam et al., 2016)

Df(2R)BSC429/BEAF-32^AB-KO, sd¹² has photoreceptor cell R8 of yellow ommatidium | somatic clone phenotype

(Jukam et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Addition of BEAF-32^AB-KO/BEAF-32^AB-KO to flies with ss^transgene6 or sev^unspecified suppresses the increased proportion of yellow R8 photoreceptors to the extent that the proportion is reduced compared to controls. In the case of sev^unspecified, BEAF-32^AB-KO/BEAF-32^AB-KO reduces the proportion of yellow R8 photoreceptors to a similar level to that seen in BEAF-32^AB-KO/BEAF-32^AB-KO animals without sev^unspecified.

BEAF-32^AB-KO/BEAF-32^AB-KO retinal clones have suppression of the increased proportion of yellow R8 photoreceptors in flies with sav^GMR.PT and wts^GMR.PT, to the extent that the proportion is reduced compared to wild-type controls.

sd¹²/sd¹² retinal clones have suppression of the increased proportion of pale R8 photoreceptors in flies with BEAF-32^AB-KO/Df(2R)BSC429, to the extent that the proportion is reduced compared to wild-type controls.

(Jukam et al., 2016)

Homozygosity for BEAF-32^AB-KO significantly increases the eye colour in flies carrying P{enh(loxP.ME)w.RW+}.

(Sultana et al., 2011)

Eye colour: flies carrying P{w^{MM.scs'.scs.cCa}} have darker pigmentation in the eyes if they are also homozygous for BEAF-32^AB-KO (in 3/4 of P{w^{MM.scs'.scs.cCa}} insertion lines tested).

Body colour: P{2scs'-2scs'} flies have darker pigmentation in the abdomen if they are also homozygous for BEAF-32^AB-KO.

Body colour: the level of pigmentation in the abdomen of P{gyp-gyp} or P{scs-2scs'} flies is unaffected if they are also homozygous for BEAF-32^AB-KO.

Eye colour: the eye pigmentation level of w^a and w^e flies is not affected by BEAF-32^AB-KO.

(Roy et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

BEAF-32^AB-KO is rescued by BEAF-32^+t5

(Jukam et al., 2016)

BEAF-32^AB-KO is rescued by BEAF-32^EGFP

(Roy et al., 2007)

BEAF-32^AB-KO is rescued by BEAF-32^+t5

(Roy et al., 2007)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Separable from: Tofu¹.

(McKowen and Hart, 2022)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

BEAF-32^AB-KO

(Jukam et al., 2016, Merzetti et al., 2013, Roy et al., 2007)

BEAF32^ABKO

(Deignan et al., 2016)

BEAF^AB-KO

(McKowen and Hart, 2022, Avva and Hart, 2016, Deignan et al., 2016, Jukam et al., 2016, Hart, 2014, Roy et al., 2011, Sultana et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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