FlyBase Allele Report: Dmel\Hs6st[UAS.cKa]

General Information

Symbol

Dmel\Hs6st^UAS.cKa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Kamimura

FlyBase ID

FBal0212983

Feature type

allele

Associated gene

Dmel\Hs6st

Associated Insertion(s)

Carried in Construct

P{UAS-Hs6st.K}

Also Known As

UAS-Hs6st

Key Links

Transgenic product class

wild_type

(Kamimura et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Kamimura et al., 2006)

Mutagen

in vitro construct

(Kamimura et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-Hs6st.K}

Cytology

Description

UAS regulatory sequences drive expression of Hs6st coding sequences.

(Kamimura et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Hs6st

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size, with Scer\GAL4^hh.PU

(Kamimura et al., 2011)

partially lethal - majority die, with Scer\GAL4^Act.PU

(Kamimura et al., 2011)

partially lethal - majority die, with Scer\GAL4^hh.PU

(Kamimura et al., 2011)

Phenotype Manifest In

wing disc, with Scer\GAL4^hh.PU

(Kamimura et al., 2011)

wing margin, with Scer\GAL4^hh.PU

(Kamimura et al., 2011)

wing margin bristle, with Scer\GAL4^Act.PU

(Kamimura et al., 2011)

Detailed Description

Statement

Reference

Expression of Hs6st^Scer\UAS.cKa using Scer\GAL4^Act.PU does not result in any detectable apoptosis in the wing disc, eye-antennal disc or central nervous system.

Expression of Hs6st^Scer\UAS.cKa using Scer\GAL4^hs.PU and a 30 minute heat shock during the third larval instar stage does not result in lethality or any morphological abnormality.

Expression of Hs2st^Scer\UAS.cKa with Scer\GAL4^Act.PU results in 89% lethality, and with Scer\GAL4^hh.PU results in 88% lethality.

Expression of Hs6st^Scer\UAS.cKa using Scer\GAL4^hh.PU results in posterior wing margin notching in 14% of flies.

Expression of Hs6st^Scer\UAS.cKa using Scer\GAL4^Act.PU impairs development of the chemosensory bristles at the wing margin.

Expression of Hs6st^Scer\UAS.cKa using Scer\GAL4^hh.PU significantly decreases the size of the posterior compartment of the wing disc.

(Kamimura et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Hs6st^UAS.cKa, Scer\GAL4^Act.PU has partially lethal - majority die phenotype, suppressible by Hsepi^UAS.cKa, Scer\GAL4^Act.PU

(Kamimura et al., 2011)

Hs6st^UAS.cKa, Scer\GAL4^hh.PU has partially lethal - majority die phenotype, suppressible by Hsepi^UAS.cKa, Scer\GAL4^hh.PU

(Kamimura et al., 2011)

Other

Statement

Reference

Hs6st^UAS.cKa, Scer\GAL4^hh.PU, sfl^UAS.cKa has lethal phenotype

(Kamimura et al., 2011)

Hs6st^UAS.cKa, Scer\GAL4^hh.PU, sfl^UAS.cKa has abnormal size phenotype

(Kamimura et al., 2011)

Hs6st^UAS.cKa, Scer\GAL4^Act.PU, sfl^UAS.cKa has partially lethal - majority die phenotype

(Kamimura et al., 2011)

Phenotype Manifest In

Other

Statement

Reference

Hs6st^UAS.cKa, Scer\GAL4^hh.PU, sfl^UAS.cKa has wing disc phenotype

(Kamimura et al., 2011)

Hs6st^UAS.cKa, Hsepi^UAS.cKa, Scer\GAL4^hh.PU has anterior crossvein phenotype

(Kamimura et al., 2011)

Hs6st^UAS.cKa, Hsepi^UAS.cKa, Scer\GAL4^hh.PU has posterior crossvein phenotype

(Kamimura et al., 2011)

Hs6st^UAS.cKa, Hsepi^UAS.cKa, Scer\GAL4^hh.PU has wing margin phenotype

(Kamimura et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Simultaneous expression of Hs6st^Scer\UAS.cKa and sfl^Scer\UAS.cKa using Scer\GAL4^Act.PU does not result in any detectable apoptosis in the wing disc, eye-antennal disc or central nervous system.

Simultaneous expression of Hs6st^Scer\UAS.cKa and sfl^Scer\UAS.cKa using Scer\GAL4^hs.PU and a 30 minute heat shock during the third larval instar stage does not result in lethality or any morphological abnormality.

Co-expression of Hsepi^Scer\UAS.cKa with Hs6st^Scer\UAS.cKa using Scer\GAL4^Act.PU results in 62% lethality.

Co-expression of Hs6st^Scer\UAS.cKa with sfl^Scer\UAS.cKa using Scer\GAL4^Act.PU results in 69% lethality.

Co-expression of Hsepi^Scer\UAS.cKa with Hs6st^Scer\UAS.cKa using Scer\GAL4^hh.PU results in 57% lethality.

Co-expression of Hs6st^Scer\UAS.cKa with sfl^Scer\UAS.cKa using Scer\GAL4^hh.PU results in 100% lethality.

Co-expression of Hs6st^Scer\UAS.cKa with Hsepi^Scer\UAS.cKa using Scer\GAL4^hh.PU results in posterior wing margin notching in 4% of flies, loss of the anterior crossvein in 4 % of flies, and ectopic posterior cross vein tissue in 29% of flies.

Co-expression of sfl^Scer\UAS.cKa and Hs6st^Scer\UAS.cKa using Scer\GAL4^hh.PU significantly decreases the size of the posterior compartment of the wing disc.

(Kamimura et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^btl.PS/Hs6st^UAS.cKa rescues Hs6st^d770

(Kamimura et al., 2006)

Comments

The small tracheoblast phenotype of Hs6st^d770 mutants is completely rescued by expression of Hs6st^Scer\UAS.cKa under the control of Scer\GAL4^btl.PS.

(Kamimura et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Hs6st^Scer\UAS.cKa

Hs6st^UAS.cKa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Kamimura

Secondary FlyBase IDs

References (2)

Report Sections

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