UASt regulatory sequences drive expression of an inverted repeat.
Adulthood-only expression of ttkGD4414 under the control of Scer\GAL4esg-NP5130 (using Gal80[ts] for the temporal control of expression) leads to a severe increase in the number of enteroendocrine cells in the adult midgut, as compared to controls.
Adults expressing ttkGD4414 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Expression of ttkGD4414 in the eye under the control of Scer\GAL4GMR.PF has a very mild effect on eye development and ommatidial arrangement.
Expression under the control of Scer\GAL4pnr-MD237 results in external sensory organs consisting of empty or multiple sockets in 20-30% of the Scer\GAL4pnr-MD237 expression domain of the notum.
Scer\GAL4NP5130, ttkGD4414 has increased cell number | adult stage phenotype, non-suppressible by phylGD12579/Scer\GAL4NP5130
ttkGD4414/Scer\GAL4Act.PU is a suppressor of decreased cell number | somatic clone | adult stage phenotype of sina2
ttkGD4414, pebEP55, Scer\GAL4GMR44E10 is a non-suppressor of abnormal oviposition phenotype of Scer\GAL4GMR44E10, ctUAS.cPa
Scer\GAL4NP5130, ttkGD4414 has enteroendocrine cell | adult stage phenotype, non-suppressible by phylGD12579/Scer\GAL4NP5130
Scer\GAL4NP5130, ttkGD4414 has adult midgut epithelium phenotype, non-suppressible by phylGD12579/Scer\GAL4NP5130
ttkGD4414/Scer\GAL4Act.PU is a suppressor of adult midgut | somatic clone phenotype of sina2
ttkGD4414/Scer\GAL4Act.PU is a suppressor of enteroendocrine cell | somatic clone | adult stage phenotype of sina2
ttkGD4414/Scer\GAL4lz-gal4 is a suppressor of photoreceptor cell R1 | third instar larval stage phenotype of msi1, sina3/sina2
ttkGD4414/Scer\GAL4lz-gal4 is a suppressor of photoreceptor cell R6 | third instar larval stage phenotype of msi1, sina3/sina2
ttkGD4414/Scer\GAL4lz-gal4 is a suppressor of photoreceptor cell R7 | third instar larval stage phenotype of msi1, sina3/sina2
ttkGD4414, pebEP55, Scer\GAL4GMR44E10 is a non-suppressor of egg chamber | oogenesis stage S14 phenotype of Scer\GAL4GMR44E10, ctUAS.cPa
MEP-1GD7728, Scer\GAL4GMR.PF, ttkGD4414 has eye phenotype
The clonal expression of ttkGD4414 under the control of Scer\GAL4Act.PU suppresses the absence of enteroendocrine cells in adult midgut sina2 clones, even leading to a severe increase in number as compared to control clones.
The severely increased number of enteroendocrine cells in the adult midgut resulting from the adulthood-only expression of ttkGD4414 under the control of Scer\GAL4esg-NP5130 (using Gal80[ts]) is not suppressed by the co-expression of phylGD12579.
Co-expression of ttkGD4414 and MEP-1GD7728 in the eye under the control of Scer\GAL4GMR.PF strongly affects ommatidial organisation, and a rough-eye phenotype is observed.