UASt regulatory sequences drive expression of an inverted repeat.
Expression under the control of Scer\GAL41407 results in an extreme increase in neuroblast size in the larval brain, without loss of neuroblasts.
Expression under the control of Scer\GAL41407 results in shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls.
Expression under the control of Scer\GAL41407 results in mild defects in the shape of neuroblasts in the larval brain.
Multinucleate spermatids are formed in males expressing scraGD9720 under the control of Scer\GAL4bam.T:Hsim\VP16, indicating a defect in cytokinesis. Only 4.4% of spermatids are mononucleate in these animals, 16.7% have two nuclei per cell (indicating failure of cytokinesis during meiosis I or meiosis II) and 78.9% have four nuclei per cell (indicating failure of cytokinesis during both meiosis I and II).
No obvious effects are seen on polarity, elongation or differentiation of spermatid cysts in males expressing scraGD9720 under the control of Scer\GAL4bam.T:Hsim\VP16, however, investment cone defects are seen.
Expression of scraGD9720 under the control of Scer\GAL4GR1 results in a significant increase in the number of binucleate follicle cells compared to control egg chambers. The increase in binucleate follicle cells is accompanied by a significant decrease in the average number of follicle cell intercellular bridges per nucleus.
Adults expressing scraGD9720 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Depending on the insertion line used, expression under the control of Scer\GAL4Mef2.PR can result in viable flies or undefined lethality.
Expression under the control of Scer\GAL4pnr-MD237 may result in semi-lethality, depending on the insertion line used.
Expression under the control of Scer\GAL4pnr-MD237 results in the absence of 0% or 70-80% of the Scer\GAL4pnr-MD237-expressing area of the notum, depending on the insertion line used.
Expression under the control of Scer\GAL4pnr-MD237 may result in mild thorax closure defects, depending on the insertion line used.
Scer\GAL4VP16.bam, scraGD9720 has abnormal cytokinesis | male phenotype, suppressible | partially by shgUbi-p63E.sgGFP
scraGD9720, Scer\GAL4repo.PU is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Pi3K92EUAS.Tag:MYC,Tag:PM(hKRAS), Scer\GAL4repo.PU, btl::Egfrλ.UAS
scraGD9720, Scer\GAL4repo.PU is a suppressor of neoplasia | third instar larval stage phenotype of Pi3K92EUAS.Tag:MYC,Tag:PM(hKRAS), Scer\GAL4repo.PU, btl::Egfrλ.UAS
Scer\GAL4VP16.bam, scraGD9720 has spermatid phenotype, suppressible | partially by shgUbi-p63E.sgGFP
scraGD9720, Scer\GAL4repo.PU is a suppressor of embryonic/larval brain | third instar larval stage phenotype of Pi3K92EUAS.Tag:MYC,Tag:PM(hKRAS), Scer\GAL4repo.PU, btl::Egfrλ.UAS
scraGD9720, Scer\GAL4repo.PU is a suppressor of larval brain neuropil glial cell | third instar larval stage phenotype of Pi3K92EUAS.Tag:MYC,Tag:PM(hKRAS), Scer\GAL4repo.PU, btl::Egfrλ.UAS
The cytokinesis defects that are seen in the spermatids of males expressing scraGD9720 under the control of Scer\GAL4bam.T:Hsim\VP16 are significantly suppressed by co-expression of shgUbi-p63E.T:Avic\GFP-rs such that 39.1% of spermatids are mononucleate, 19.3% are binucleate and 41.6% have four nuclei.