Expression of Pburs^GD6814 under the control of Scer\GAL4^nSyb.PU (in combination with UAS-Dicer2 to increase RNAi efficiency) results in a significant increase in the latency to sperm ejection in females after the end of copulation.

In contrast to wild type flies placed in a confined environment, newly emerged flies expressing Pburs^GD6814 under the control of Scer\GAL4^Burs.PP do not show wing expansion upon stimulation of the CCAP-expressing neurons. Approximately 30% of the mutant flies do show abdominal flexion (a behavioural signature of wing expansion), but only after environmental delays similar to those of flies in which the CCAP-expressing neurons are not activated.

Newly emerged flies expressing Pburs^GD6814 in the B[[AG]] neurons (the intersection of the Hsim\VP16⁹⁹ and Scer\GAL4^{DBD.Burs.T:Zzzz\Zip-} split-gal4 hemidrivers) and placed in a confined environment still show the abdominal flexion behaviours characteristic of wing expansion, although the wings typically undergo little to no expansion during the abdominal flexion.

Expression of pburs^GD6814 under the control of Scer\GAL4^dpp.blk1 results in multiple cellular hairs in the wing.

Expression of pburs^GD6814 under the control of Scer\GAL4^en-e16E results in multiple cellular hairs in the wing.

Expression of pburs^GD6814 under the control of Scer\GAL4^ap-md544 results in multiple cellular hairs in the wing.

Adults expressing Pburs^GD6814 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.