FlyBase Allele Report: Dmel\lwr[GD10017]

General Information

Symbol

Dmel\lwr^GD10017

Species

D. melanogaster

Name

FlyBase ID

FBal0209954

Feature type

allele

Associated gene

Dmel\lwr

Associated Insertion(s)

Carried in Construct

P{GD10017}

Also Known As

lwr RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD10017}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD10017

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage, with Scer\GAL4^C-765

(Zhang et al., 2017)

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^ey.PU

(Kanakousaki and Gibson, 2012)

visible | adult stage, with Scer\GAL4^Bx-MS1096

(Zhang et al., 2017)

visible | adult stage, with Scer\GAL4^C-765

(Zhang et al., 2017)

Phenotype Manifest In

adult head, with Scer\GAL4^ey.PU

(Kanakousaki and Gibson, 2012)

eye, with Scer\GAL4^ey.PU

(Kanakousaki and Gibson, 2012)

eye disc, with Scer\GAL4^ey.PU

(Kanakousaki and Gibson, 2012)

wing, with Scer\GAL4^Bx-MS1096

(Zhang et al., 2017)

wing, with Scer\GAL4^C-765

(Zhang et al., 2017)

Detailed Description

Statement

Reference

Expression of lwr^GD10017 under the control of Scer\GAL4^Bx-MS1096 leads to severe wing morphology defects with rudimentary deformed wings, expression driven by Scer\GAL4^C-765 results in in smaller wings with mildly crinkled appearance.

(Zhang et al., 2017)

Expression of lwr^GD10017 under the control of Scer\GAL4^GMR.PF does not result in a mutant phenotype in the eye.

Expression of lwr^GD10017 under the control of Scer\GAL4^ey.PU results in a dramatic reduction in the size of the eye disc and severely reduced adult eyes. In many cases, head formation is abolished and the animals do not eclose.

(Kanakousaki and Gibson, 2012)

Adults expressing lwr^GD10017 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in viable flies or late pupal lethality.

(Schnorrer et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

10017

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Scer\GAL4^C-765/lwr^GD10017 is an enhancer of visible | adult stage phenotype of Scer\GAL4^C-765, smo^PKA12.UAS

(Zhang et al., 2017)

Phenotype Manifest In

Enhancer of

Statement

Reference

Scer\GAL4^C-765/lwr^GD10017 is an enhancer of wing phenotype of Scer\GAL4^C-765, smo^PKA12.UAS

(Zhang et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The wing defects (partial fusion of veins L3 and L4) characteristic for flies expressing smo^{PKA12.Scer\UAS} under the control of Scer\GAL4^C-765 are strongly exacerbated by co-expression of lwr^GD10017 (which on its own induces moderately reduced wing size and crinkled appearance) leading to small severely misshapen wings.

(Zhang et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v33685

w¹¹¹⁸; P{GD10017}v33685

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

10017

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

lwr^GD10017

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections

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