UASt regulatory sequences drive expression of an inverted repeat.
Expression of gloGD12082 under the control of Scer\GAL4GMR.PU has no obvious effect on adult eye morphology, whereas expression driven by Scer\GAL4elav.PU results in significant decrease in adult climbing ability.
Adults expressing gloGD12082 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies.
Expression under the control of Scer\GAL4Mef2.PR results in early pupal lethality.
Expression under the control of Scer\GAL4pnr-MD237 results in loss of bristles on the notum in 30-40% of the Scer\GAL4pnr-MD237 expression domain.
Expression under the control of Scer\GAL4pnr-MD237 results in the absence of 50-60% of the Scer\GAL4pnr-MD237-expressing area of the notum.
Expression of gloGD12082 under the control of Scer\GAL4477 results in a reduction in the density of terminal dendritic branches of class IV da neurons in third instar larvae.
Scer\GAL4elav.PU, gloGD12082 has abnormal locomotor behavior | adult stage phenotype, enhanceable by TBPHRNAi.UAS.cUa/Scer\GAL4elav.PU/TBPH[+]/TBPHΔ23
Scer\GAL4elav.PU/gloGD12082 is an enhancer of abnormal locomotor behavior | adult stage phenotype of Scer\GAL4elav.PU, TBPHΔ23, TBPHRNAi.UAS.cUa
gloGD12082/Scer\GAL4GMR.PU is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4GMR.PU, TBPHUAS.Tag:FLAG
gloGD12082/Scer\GAL4GMR.PU is a suppressor | partially of melanotic mass phenotype | adult stage phenotype of Scer\GAL4GMR.PU, TBPHUAS.Tag:FLAG
gloGD12082/Scer\GAL4GMR.PU is a suppressor | partially of abnormal eye color phenotype of Scer\GAL4GMR.PU, TBPHUAS.Tag:FLAG
gloGD12082/Scer\GAL4GMR.PU is a suppressor | partially of eye phenotype of Scer\GAL4GMR.PU, TBPHUAS.Tag:FLAG
gloGD12082/Scer\GAL4GMR.PU is a suppressor | partially of ommatidium | adult stage phenotype of Scer\GAL4GMR.PU, TBPHUAS.Tag:FLAG
The climbing ability deficit observed both in the TBPH hypomorphic flies (TBPHΔ23/+;Scer\GAL4elav.PU>TBPHdsRNA.Scer\UAS.cUa, UAS-Dicer2) and flies expressing gloGD12082 (also with UAS-Dicer2 and under Scer\GAL4elav.PU control) is much enhanced when the two mutants are combined.
The eye degeneration phenotype (appearance of melanotic spots, fusion of ommatidia) characteristic for adult flies expressing TBPHScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4GMR.PU is strongly suppressed by co-expression of gloGD12082.