FB2024_03 , released June 25, 2024
Allele: Dmel\MycGD1419
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General Information
Symbol
Dmel\MycGD1419
Species
D. melanogaster
Name
FlyBase ID
FBal0209363
Feature type
allele
Associated gene
Dmel\Myc
Associated Insertion(s)
Carried in Construct
P{GD1419}
Also Known As
UAS-dmyc-RNAi, UAS-Myc-RNAi, UAS-dMycdsRNA, dMycdsRNA, dMYC RNAi
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Dickson et al., 2007.7.18)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Dickson et al., 2007.7.18)
Mutagen
in vitro construct
(Dickson et al., 2007.7.18)
Progenitor genotype
Carried in construct
P{GD1419}
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-GD1419
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  cancer
      CEA
      (Qiao et al., 2020)
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  cancer
      modeled by ykiUAS.cHa
      (Ziosi et al., 2010)
      ameliorates  high grade glioma
      modeled by Wdr62UAS.Tag:HA
      (Shohayeb et al., 2020)
      ameliorates  brain cancer
      modeled by bratHMS01121
      (Diaz et al., 2024)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of MycGD1419 under the control of Scer\GAL4repo.PU leads to a decrease in the brain volume, glial cell size and glial nucleolus size in third instar larvae.

      (Shohayeb et al., 2020)

      Expressing MycGD1419 under the control of Scer\GAL4ptc-559.1 results in small, underdeveloped larval salivary glands, as compared to controls; there is a severe decrease in DNA content.

      (Yanku et al., 2018)

      Scer\GAL4Cg.PA MycGD1419 animals reach pupation with a delay of 48h at their third instar transition; these animals are smaller throughout development, and eclose 2 days later than controls as smaller, lighter adults. Scer\GAL4Cg.PA MycGD1419 flies have small wings, owing to decreased size and number of cells. Scer\GAL4Cg.PA MycGD1419 flies also have eyes with smaller and fewer ommatidia. Scer\GAL4ppl.PU MycGD1419 flies have smaller wings comprising smaller cells.

      Scer\GAL4Hml.PG-mediated expression of MycGD1419 does not affect developmental timing or body/wing size.

      Fat cells in the fat bodies of Scer\GAL4Cg.PA MycGD1419 animals contain cytoplasmic lipid droplets larger in size than controls. The fat cells are smaller and contain less triglyceride compared to controls.

      The half-life of Scer\GAL4Cg.PA MycGD1419 animals during starvation is decreased compared to controls.

      (Parisi et al., 2013)

      Over-expression of dmGD1419 using Scer\GAL4sca-109-68 decreases the nuclear and nucleolar size of the posterior scutellar shaft cells.

      (Kawamori and Yamaguchi, 2011)

      Adults expressing MycGD1419 under the control of the cardioblast-specific Scer\GAL4tin.CΔ4 driver show significantly reduced survival on day 6 after a shift to 29[o]C compared to control flies.

      (Neely et al., 2010)

      Adults expressing MycGD1419 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

      (Neely et al., 2010)

      Depending on the insertion line used, expression under the control of Scer\GAL4Mef2.PR can result in early pupal lethality or late larval lethality.

      Expression under the control of Scer\GAL4Mef2.PR results in grossly normal larval body wall muscles.

      Expression under the control of Scer\GAL4Mef2.PR results in wild-type sarcomeres in the larval body wall muscles.

      (Schnorrer et al., 2010)

      Mosaic clones expressing dmGD1419 under the control of Scer\GAL4αTub84B.PP exhibit a reduced number of cells with respect to those observed in wild-type clones.

      (Ziosi et al., 2010)

      Expression under the control of Scer\GAL4pnr-MD237 results in lethality (either before or at the pupal stage), depending on the insertion line used.

      (Mummery-Widmer et al., 2009)
      External Data
      Linkouts
      Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Enhanced by
      NOT suppressed by
      Suppressor of
      Other
      Phenotype Manifest In
      NOT Enhanced by
      NOT suppressed by
      Suppressor of
      NOT Suppressor of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The co-expression of HUWE1GD13830 and MycGD1419 under the control of Scer\GAL4Hey.PO (in combination with Dicer-2, for an efficient RNAi) results in small cells in the larval salivary gland.

      (Yanku et al., 2018)

      Expression of dmGD1419 is unable to suppress the dorsal-ventral boundary defects seen when banC.Scer\UAS.T:Avic\GFP-EGFP is expressed under the control of Scer\GAL4sd-SG29.1.

      (Becam et al., 2011)

      Expression of MycGD1419 under the control of Scer\GAL4tub.PU suppresses the cell polarity defects seen in l(2)gl4 mutant wing disc clones generated in a Minute background, even when the mutant clone tissue occupies a larger proportion of the disc. No degradation of the basement membrane occurs and clones do not form tumorous masses, either in the wing pouch or proximal (hinge and pleura) regions.

      (Froldi et al., 2010)
      Xenogenetic Interactions
      Statement
      Reference

      Expression of BacA\p35Scer\UAS.cHa in wing disc clones expressing dmGD1419 under the control of Scer\GAL4Act5C.PP results in reduced nucleolar cell size. Mutant cells display a rounded-morphology.

      (Mitchell et al., 2010)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      MycGD1419
      dmGD1419
      Name Synonyms
      Secondary FlyBase IDs
        References (27)