FlyBase Allele Report: Dmel\bun[GD1392]

General Information

Symbol

Dmel\bun^GD1392

Species

D. melanogaster

Name

FlyBase ID

FBal0209195

Feature type

allele

Associated gene

Dmel\bun

Associated Insertion(s)

Carried in Construct

P{GD1392}

Also Known As

bun^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD1392}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD1392

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult midgut, with Scer\GAL4^Delta.PU

(Nie et al., 2015)

adult midgut, with Scer\GAL4^NP5130

(Nie et al., 2015)

adult mushroom body alpha-lobe, with Scer\GAL4^ey-OK107

(Kim et al., 2009)

adult mushroom body beta-lobe, with Scer\GAL4^ey-OK107

(Kim et al., 2009)

enteroblast | adult stage, with Scer\GAL4^NP5130

(Nie et al., 2015)

enteroendocrine cell | adult stage, with Scer\GAL4^NP5130

(Nie et al., 2015)

intestinal stem cell | adult stage, with Scer\GAL4^NP5130

(Nie et al., 2015)

mesothoracic tergum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Expression of bun^GD1392 under the control of Scer\GAL4^esg-NP5130 (in combination with a Gal80[ts] transgene to restrict expression to the adult stage) results in defects in the adult midgut, including a reduction in the number of mitotic cells, decreased numbers of intestinal stem cells and enteroblasts and remaining cells were abnormally round-shaped; these flies also exhibit reduced numbers of enteroendocrine cells, but do not exhibit defects in the normal intestinal stem cell-enteroblast asymmetry, nor any significant change in the size of intestinal stem cells, as compared to controls.

Expression of bun^GD1392 under the control of Scer\GAL4^Dl.PU (in combination with a Gal80[ts] transgene to restrict expression to the adult stage) results in a significant reduction in the number of mitotic cells, as compared with controls.

(Nie et al., 2015)

Adults expressing bun^GD1392 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression of bun^GD1392 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies or partial lethality, depending on the particular P{GD1392} insertion line used.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in late pupal lethality.

(Schnorrer et al., 2010)

Scer\GAL4^ey-OK107-mediated expression of bun^GD1392 results in thinner alpha/beta lobes than wild type.

(Kim et al., 2009)

Expression under the control of Scer\GAL4^pnr-MD237 may result in semi-lethality, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 0% or 70-80% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 may result in mild thorax closure defects, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1392

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Scer\GAL4^NP5130, bun^GD1392 has decreased occurrence of cell division | adult stage phenotype, non-suppressible by BacA\p35^UAS.cUa, Scer\GAL4^NP5130

(Nie et al., 2015)

Suppressor of

Statement

Reference

bun^GD1392/Scer\GAL4^NP5130 is a suppressor of increased occurrence of cell division | adult stage phenotype of Scer\GAL4^NP5130, msn^JF03219

(Nie et al., 2015)

bun^GD1392/Scer\GAL4^NP5130 is a suppressor of increased cell size | adult stage phenotype of Scer\GAL4^NP5130, gig^GD1454

(Nie et al., 2015)

bun^GD1392/Scer\GAL4^NP5130 is a suppressor of increased occurrence of cell division | adult stage phenotype of Scer\GAL4^NP5130, hpo^JF02740

(Nie et al., 2015)

bun^GD1392/Scer\GAL4^NP5130 is a suppressor of increased occurrence of cell division | adult stage phenotype of Scer\GAL4^NP5130, hpo^KK101704

(Nie et al., 2015)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Scer\GAL4^NP5130, bun^GD1392 has adult midgut phenotype, non-suppressible by BacA\p35^UAS.cUa, Scer\GAL4^NP5130

(Nie et al., 2015)

Suppressor of

Statement

Reference

bun^GD1392/Scer\GAL4^NP5130 is a suppressor of adult midgut phenotype of Scer\GAL4^NP5130, hpo^JF02740

(Nie et al., 2015)

bun^GD1392/Scer\GAL4^NP5130 is a suppressor of adult midgut phenotype of Scer\GAL4^NP5130, hpo^KK101704

(Nie et al., 2015)

bun^GD1392/Scer\GAL4^NP5130 is a suppressor of adult midgut phenotype of Scer\GAL4^NP5130, msn^JF03219

(Nie et al., 2015)

bun^GD1392/Scer\GAL4^NP5130 is a suppressor of adult midgut phenotype of Scer\GAL4^NP5130, gig^GD1454

(Nie et al., 2015)

bun^GD1392/Scer\GAL4^NP5130 is a suppressor of intestinal stem cell | adult stage phenotype of Scer\GAL4^NP5130, gig^GD1454

(Nie et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of bun^GD1392 suppresses the increased intestinal stem cell size seen in flies expressing gig^GD1454 under the control of Scer\GAL4^esg-NP5130.

Co-expression of bun^GD1392 suppresses the increased number of mitotic cells seen in the midguts of flies expressing msn^JF03219, vn^Scer\UAS.cUa, upd1^Scer\UAS.cUa, N^DN.Scer\UAS, InR^{A1325D.Scer\UAS}, upd3^{Scer\UAS.P\T.cZa}, Egfr^{U.A887T.Scer\UAS}, hpo^KK101704, or hpo^JF02740 under the control of Scer\GAL4^esg-NP5130.

(Nie et al., 2015)

Xenogenetic Interactions

Statement

Reference

Co-expression of BacA\p35^Scer\UAS.cUa does not rescue the decreased cell proliferation observed in the midguts of flies expressing bun^GD1392 under the control of Scer\GAL4^esg-NP5130.

(Nie et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v19680

w¹¹¹⁸ P{GD1392}v19680

Notes on Origin

Discoverer

Comments

"bun[RNAi]" in FBrf0208074 could refer to P{GD10387} or P{GD1392}.

(Kim et al., 2009)

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1392

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

bun^GD1392

bun^RNAi

(Nie et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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