FlyBase Allele Report: Dmel\Rab7[GD11800]

General Information

Symbol

Dmel\Rab7^GD11800

Species

D. melanogaster

Name

FlyBase ID

FBal0208211

Feature type

allele

Associated gene

Dmel\Rab7

Associated Insertion(s)

Carried in Construct

P{GD11800}

Also Known As

Rab7 RNAi, UAS-Rab7 RNAi, Rab7^RNAi, UAS rab7-RNAi, UAS-rab7-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD11800}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD11800

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult gut, with Scer\GAL4^NP0001

(Nassari et al., 2022)

adult midgut epithelium, with Scer\GAL4^esg.PU

(Zhang et al., 2019)

adult midgut epithelium, with Scer\GAL4^NP5130

(Nagy et al., 2016)

autophagosome | larval stage | somatic clone, with Scer\GAL4^Act.PU

(Lőrincz et al., 2017)

embryonic/larval fat body | larval stage | somatic clone, with Scer\GAL4^Act.PU

(Lőrincz et al., 2017)

endosome, with Scer\GAL4^hh-Gal4

(Yousefian et al., 2013)

endosome, with Scer\GAL4^ptc-559.1

(Yousefian et al., 2013)

enteroblast of posterior adult midgut epithelium, with Scer\GAL4^NP5130

(Nagy et al., 2016)

eye disc, with Scer\GAL4^da.G32

(Yuva-Aydemir et al., 2011)

garland cell, with Scer\GAL4^pros.PU

(Lőrincz et al., 2019)

glial cell, with Scer\GAL4^da.G32

(Yuva-Aydemir et al., 2011)

intestinal stem cell of posterior adult midgut epithelium, with Scer\GAL4^NP5130

(Nagy et al., 2016)

late endosome, with Scer\GAL4^pros.PU

(Lőrincz et al., 2019)

lysosome, with Scer\GAL4^pros.PU

(Lőrincz et al., 2019)

multivesicular body, with Scer\GAL4^ptc-559.1

(Yousefian et al., 2013)

Detailed Description

Statement

Reference

Garland cells expressing Rab7^GD11800 under the control of Scer\GAL4^pros.PU show enlarged late endosomes and abnormal lysosomes.

(Lőrincz et al., 2019)

Adulthood-only expression of Rab7^GD11800 under the control of Scer\GAL4^esg.PU leads to a significant increase in the number of mitotic cells (phospho-H3 staining) in the adult midgut, as compared to controls. (The temporal regulation of expression is dependent on Gal80[ts] and induced by temperature shift to 29[o]C for 5-6 days during adulthood).

(Zhang et al., 2019)

Expression of Rab7^GD11800 under the control of Scer\GAL4^Act.PU in clones of larval fat body cells prevents formation of autolysosome.

(Lőrincz et al., 2017)

Animals expressing Rab7^GD11800 under the control of Scer\GAL4^grh.D4 are viable, no obvious defects in larval dorsal trunk elongation and airway morphology are observed and the larvae are of comparable size to age-matched controls.

(Wingen et al., 2017)

The adulthood-only expression of Rab7^GD11800 under the control of Scer\GAL4^esg-NP5130 (and Gal80[ts], for the temporal control of expression) leads to a significant increase in the numbers of both intestinal stem cells and enteroblasts in the adult midgut, as compared to controls.

(Nagy et al., 2016)

Expression of Rab7^GD11800 under the control of Scer\GAL4^hh-Gal4 or Scer\GAL4^ptc-559.1 results in an enlarged endosome phenotype.

Expression of Rab7^GD11800 under the control of Scer\GAL4^hh-Gal4 in the posterior compartment of the wing disc results in an increase in perimeter of the multivesicular bodies in the posterior compartment compared to those in the anterior compartment.

(Yousefian et al., 2013)

Primary macrophages isolated from animals expressing Rab7^GD11800 under the control of Scer\GAL4^Pxn.PS have an similar number of F-actin rich protrusions to wild type.

(Jean et al., 2012)

Expression of Rab7^GD11800 under the control of Scer\GAL4^da.G32 results in a glial overmigration phenotype in the eye disc.

Expression of Rab7^GD11800 under the control of Scer\GAL4^repo.PU or Scer\GAL4^ey.3.5.Exel has no effect on glial cell migration in the eye disc.

(Yuva-Aydemir et al., 2011)

Adults expressing Rab7^GD11800 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in lethality by 7 days after eclosion.

(Schnorrer et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

11800

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Rab7^GD11800, Scer\GAL4^esg.PU has increased occurrence of cell division | adult stage phenotype, suppressible by Rab11^JF02812, Scer\GAL4^esg.PU

(Zhang et al., 2019)

Suppressor of

Statement

Reference

Scer\GAL4^nSyb.PU/Rab7^GD11800 is a suppressor of abnormal neurophysiology | third instar larval stage phenotype of sky¹/sky²

(Fernandes et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

Rab7^GD11800, Scer\GAL4^esg.PU has adult midgut epithelium phenotype, suppressible by Rab11^JF02812, Scer\GAL4^esg.PU

(Zhang et al., 2019)

Suppressor of

Statement

Reference

Rab7^GD11800, Scer\GAL4^Act.PU is a suppressor | somatic clone of embryonic/larval fat body | somatic clone | larval stage phenotype of Rab2^{Q65L.UASp.YFP}, Scer\GAL4^Act.PU

(Lőrincz et al., 2017)

Rab7^GD11800, Scer\GAL4^Act.PU is a suppressor | somatic clone of autophagosome | somatic clone | larval stage phenotype of Rab2^{Q65L.UASp.YFP}, Scer\GAL4^Act.PU

(Lőrincz et al., 2017)

Scer\GAL4^hh-Gal4/Rab7^GD11800 is a suppressor of phenotype of l(2)gd1^d7

(Schneider et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

The formation of abnormally large autophagic structures in the clones of larval fat body cells expressing Rab2^{Q65L.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^Act.PU is prevented by co-expression of Rab7^GD11800.

(Lőrincz et al., 2017)

The increase in excitatory junctional current amplitude at the neuromuscular junction which is seen in sky¹/sky² larvae is suppressed if they are also expressing Rab7^GD11800 under the control of Scer\GAL4^nSyb.PU. However, the increased synaptic vesicle trafficking to endosomes seen at the neuromuscular junction in the sky¹/sky² larvae is not suppressed.

(Fernandes et al., 2014)

Scer\GAL4^hh-Gal4-mediated expression of Rab7^GD11800 suppresses the ectopic N activation phenotype (ectopic expression of wg) in the posterior of l(2)gd1^d7 wing discs.

Expression of Rab7^GD11800 does not suppress the ectopic N activation phenotype in shrb⁴ follicle cell clones.

(Schneider et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v40337

w¹¹¹⁸; P{GD11800}v40337

v40338

w¹¹¹⁸; P{GD11800}v40338

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

11800

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Rab7_dsRNA

(Yuva-Aydemir et al., 2011)

Rab7^GD11800

Rab7^RNAi

(Fernandes et al., 2014)

rab7^RNAi

(Lőrincz et al., 2019)

Name Synonyms

Secondary FlyBase IDs

References (33)

Report Sections

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