UASt regulatory sequences drive expression of an inverted repeat.
Expression of Ncc69GD14863 in glial cells under the control of Scer\GAL4repo.PU causes substantial bang-sensitive paralysis. The threshold to evoked seizure-like discharges in these flies is reduced compared to controls. No bang-sensitive paralysis is observed when Ncc69GD14863 is expressed in neurons under the control of Scer\GAL4elav-C155.
Expression of Ncc69GD14863 under the control of Scer\GAL4repo.PU causes nerve swelling. Third instar larval abdominal nerves display an increase in the average never cross-sectional area compared to controls.
Expression of Ncc69GD14863 in the subperineurial glia under the control of Scer\GAL4Gli-rL82.29 results in bulges in the abdominal nerves of third instar larvae.
Expression of Ncc69GD14863 in the neurons under the control of Scer\GAL4elav-C155 has no effect on the morphology of the abdominal nerves of third instar larvae.
Adults expressing Ncc69GD14863 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Scer\GAL4c747/Ncc69GD14863 is a suppressor of paralytic | recessive phenotype of kccDHS1
The bang-sensitive paralytic phenotype of homozygous kccDHS1 mutants is markedly suppressed by the expression of Ncc69GD14863 via Scer\GAL4c747.