UASt regulatory sequences drive expression of an inverted repeat.
Adults expressing HnGD12227 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
DdcGD1189, HnGD12227, Scer\GAL4G0431 has abnormal memory phenotype
DdcGD1189, HnGD12227, Scer\GAL4c316, Scer\GAL80ts.αTub84B has abnormal memory phenotype
1 day memory is impaired after spaced training, but not after massed training in an olfactory associative conditioning assay in flies co-expressing DdcGD1189 and HnGD12227 under the control of Scer\GAL4G0431. The defect in memory after spaced training is still seen if the temperature sensitive Scer\GAL80ts.αTub84B allele is used to restrict Scer\GAL4 expression to the adult stage.
Temporal induction after development of both DdcKK100293 and HnGD12227 in Scer\GAL4c316 neurons impairs anesthesia-resistant memory formation.