UASt regulatory sequences drive expression of an inverted repeat.
Expression of Fmr1GD1288 results in learning and memory defects reminiscent of those observed in fragile X syndrome patients.
The expression of Fmr1GD1288 under the control of Scer\GAL4c135 (one driver copy) or Scer\GAL4upd1.PU (one driver copy), but not of Scer\GAL4nos.PU (one or two driver copies), leads to the formation of crystalline aggregates (of Stellate protein) in spermatocytes, which are absent in control spermatocytes.
Expression of Fmr1GD1288 under the control of Scer\GAL4elav.PU (in combination with GAL80ts to limit expression to adult stage) results in learning and memory defects as compared to controls in a negatively reinforced olfactory learning paradigm. Learning defects are reversed by feeding flies with the pyrrolidinone Rolipram.
Expression of Fmr1GD1288 under the control of Scer\GAL4c747 or Scer\GAL4Mef2.247 results in learning defects as compared to controls in a negatively reinforced olfactory learning paradigm.
Expression of Fmr1GD1288 under the control of Scer\GAL4c747 (in combination with GAL80ts to limit expression to adult stage) results in learning and memory defects as compared to controls in a negatively reinforced olfactory learning paradigm. Learning defects are reversed by feeding the flies 2-Methyl-6-(phenylethynyl)pyridine.
Expression of Fmr1GD1288 under the control of Fmr1GD1288, Scer\GAL4c747, Scer\GAL4Mef2.247 or Scer\GAL4c747 results in increased learning performance with 2 or 4 CS-US pairings, worse performance with 6 or 8 pairings, and similar performance with 12 pairings, compared to controls.
Expression of Fmr1GD1288 under the control of Scer\GAL4NP1131 or under the control of Scer\GAL4Alp4-c507 does not result in learning defects as compared to controls in a negatively reinforced olfactory learning paradigm.
Adults expressing Fmr1GD1288 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Fmr1GD1288, Scer\GAL4GMR.PU is an enhancer of abnormal eye color | adult stage phenotype of Hsap\TARDBPUAS.YFP.cEa, Scer\GAL4GMR.PU
Fmr1GD1288, Scer\GAL4Toll-6-D42 is an enhancer of abnormal locomotor behavior | third instar larval stage phenotype of Hsap\TARDBPUAS.YFP.cEa, Scer\GAL4Toll-6-D42
Fmr1GD1288, Scer\GAL4GMR.PU is an enhancer of abnormal eye color | adult stage phenotype of Hsap\TARDBPG298S.UAS.YFP, Scer\GAL4GMR.PU
Fmr1GD1288, Scer\GAL4Toll-6-D42 is an enhancer of abnormal locomotor behavior | third instar larval stage phenotype of Hsap\TARDBPG298S.UAS.YFP, Scer\GAL4Toll-6-D42
Fmr1GD1288, Scer\GAL4Toll-6-D42 is an enhancer of abnormal locomotor behavior | third instar larval stage phenotype of Scer\GAL4Toll-6-D42, TBPHGD6943
Fmr1GD1288, Scer\GAL4GMR.PU is an enhancer of eye | adult stage phenotype of Hsap\TARDBPUAS.YFP.cEa, Scer\GAL4GMR.PU
Fmr1GD1288, Scer\GAL4GMR.PU is an enhancer of eye | adult stage phenotype of Hsap\TARDBPG298S.UAS.YFP, Scer\GAL4GMR.PU
The longer time it takes for third instar larvae expressing TBPHGD6943 under the control of Scer\GAL4Toll-6-D42 to roll over in a turning assay compared to wild-type controls is lengthened further by co-expression of Fmr1GD1288 RNAi and, to a lesser extent, also by co-expression of Fmr1Scer\UAS.T:Avic\GFP-YFP.
The depigmentation phenotype in the adult eye and the lengthened time it takes for larvae to roll over in a turning assay characteristic for animals expressing Hsap\TARDBPScer\UAS.T:Avic\GFP-YFP.cEa under the control of Scer\GAL4GMR.PU or Scer\GAL4Toll-6-D42 respectively, are exacerbated further by co-expression of Fmr1GD1288 RNAi. However, co-expression of Hsap\TARDBPScer\UAS.T:Avic\GFP-YFP.cEa and Fmr1GD1288 under the Scer\GAL4Toll-6-D42 driver is pupal stage lethal.
The depigmentation phenotype in the adult eye and the lengthened time it takes for larvae to roll over in a turning assay characteristic for animals expressing Hsap\TARDBPG298S.Scer\UAS.T:Avic\GFP-YFP under the control of Scer\GAL4GMR.PU or Scer\GAL4Toll-6-D42 respectively, are exacerbated further by co-expression of Fmr1GD1288 RNAi. However, co-expression of Hsap\TARDBPG298S.Scer\UAS.T:Avic\GFP-YFP and Fmr1GD1288 under the Scer\GAL4Toll-6-D42 driver is pupal stage lethal.