The expression of CadN^GD161 under the control of Scer\GAL4^sev.PM181 leads to the larval medulla showing significantly disturbed columns and R7 photoreceptor axon terminals expanding toward the peripheral part of the column, but still innervating on the larval medulla neuropil, as compared with controls; R8 photoreceptor and Mi1 neuron terminals also become disorganized, expanded and overlap R7 photoreceptor terminals.

The expression of CadN^GD161 under the control of Scer\GAL4^sens.F2 leads to the larval medulla showing R8 photoreceptor terminals that are deformed and expanded to fuse with neighboring columns; R7 photoreceptor terminals also become expanded and enwrapped by R8 terminals; Mi1 neuron terminals also become expanded and overlap more with R8 photoreceptor terminals.

Clonal expression of CadN^GD161 under the control of Scer\GAL4^GMR.PC in R7 photoreceptors (induced by Scer\FLP1^G5.20C11) leads to axon terminals expanding toward the peripheral part of the column in the larval medulla, although the column morphology is largely preserved, as compared with controls.

Clonal expression of CadN^GD161 under the control of Scer\GAL4^GMR.PC in R8 photoreceptors (induced by Scer\FLP1^G5.sens.PC) leads to axon terminals expanding toward the peripheral part of the column in the larval medulla, as compared to controls.

Adults expressing CadN^GD161 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) can show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies, depending on the particular P{GD161} insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 may result in an enlarged notum, depending on the insertion line used.