FlyBase Allele Report: Dmel\Rnb[GD12299]

General Information

Symbol

Dmel\Rnb^GD12299

Species

D. melanogaster

Name

FlyBase ID

FBal0205626

Feature type

allele

Associated gene

Dmel\Rnb

Associated Insertion(s)

Carried in Construct

P{GD12299}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD12299}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD12299

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell size | adult stage, with Scer\GAL4^Pdf.PU

(Kozlov et al., 2017)

abnormal circadian rhythm, with Scer\GAL4¹¹¹⁸

(Kozlov et al., 2017)

abnormal circadian rhythm, with Scer\GAL4^Pdf.PU

(Kozlov et al., 2017)

abnormal circadian rhythm, with Scer\GAL4^tim.PU

(Kozlov et al., 2017)

abnormal circadian rhythm, with Scer\GAL4^Tub.PU

(Kozlov et al., 2017)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

adult s-LNv neuron | adult stage, with Scer\GAL4^Pdf.PU

(Kozlov et al., 2017)

dendritic tree | adult stage, with Scer\GAL4^Pdf.PU

(Kozlov et al., 2017)

Detailed Description

Statement

Reference

In adults previously entrained under 12h:12h light:dark cycles, constitutive expression of Rnb^GD12299 driven by either Scer\GAL4^tim.PU or Scer\GAL4^Pdf.PU, adulthood-only expression driven by either Scer\GAL4^Pdf.PU or Scer\GAL4¹¹¹⁸ and temporally-controlled by Gal80[ts], or expression only during development driven by Scer\GAL4^Pdf.PU and temporally-controlled by Gal80[ts], leads to significant increases in the circadian period, despite of insignificant changes in the proportion of rhythmic individuals, upon shift to constant darkness conditions, as compared to controls; constitutive expression under control of Scer\GAL4^tub.PU alone leads both to a significant increase in the circadian period and to a significant decrease in the proportion of rhythmic individuals upon shift to constant darkness conditions, as compared to controls.

In adults maintained under constant darkness conditions, the expression of Rnb^GD12299 under the control of Scer\GAL4^Pdf.PU leads to a significant increase in the dendritic tree area of s-LNv neurons, as compared to controls.

(Kozlov et al., 2017)

Adults expressing CG7837^GD12299 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12299

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Rnb^GD12299, Scer\GAL4¹¹¹⁸ has abnormal circadian rhythm phenotype, enhanceable by Hr51^RNAi.UAS.a, Scer\GAL4¹¹¹⁸

(Kozlov et al., 2017)

Enhancer of

Statement

Reference

Rnb^GD12299, Scer\GAL4¹¹¹⁸ is an enhancer of abnormal circadian rhythm phenotype of Hr51^RNAi.UAS.a, Scer\GAL4¹¹¹⁸

(Kozlov et al., 2017)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Adulthood co-expression of Rnb^GD12299 and Hr51^{miRNA.Scer\UAS.a}, driven by Scer\GAL4¹¹¹⁸ and temporally-controlled by Gal80[ts], enhances the increase in circadian period observed upon the adulthood expression of either Rnb^GD12299 alone or Hr51^{miRNA.Scer\UAS.a} alone.

(Kozlov et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v22573

w¹¹¹⁸; P{GD12299}v22573

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12299

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG7837^GD12299

Rnb^GD12299

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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