FlyBase Allele Report: Dmel\Vps35[GD11710]

General Information

Symbol

Dmel\Vps35^GD11710

Species

D. melanogaster

Name

FlyBase ID

FBal0204753

Feature type

allele

Associated gene

Dmel\Vps35

Associated Insertion(s)

Carried in Construct

P{GD11710}

Also Known As

dVPS35 RNAi, UAS-Vps35-RNAi, UAS-vps35 RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD11710}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD11710

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Parkinson's disease

CEA

(Miura et al., 2014)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Parkinson's disease

modeled by Hsap\TMEM230^{184PGext5.UAS.2}

(Ma et al., 2022)

modeled by Hsap\SNCA^UAS.cFa

(Miura et al., 2014)

ameliorates Huntington's disease

modeled by Hsap\HTT^{128Q.1-336.UAS}

(Shirasaki et al., 2012)

model of Parkinson's disease

is exacerbated by Hsap\SNCA^UAS.cFa

(Miura et al., 2014)

exacerbates amyotrophic lateral sclerosis type 8

modeled by Vap33^{P58S.UAS.Tag:FLAG}

(Sanhueza et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal circadian behavior | adult stage, with Scer\GAL4^ple.PF

(Inoshita et al., 2017)

abnormal locomotor behavior | adult stage | progressive, with Scer\GAL4^GMR.PS

(Miura et al., 2014)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^Mhc.PK

(Inoshita et al., 2017)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^Toll-6-D42

(Inoshita et al., 2017)

abnormal neurophysiology | third instar larval stage, with Scer\GAL4^Mhc.PK

(Inoshita et al., 2017)

abnormal neurophysiology | third instar larval stage, with Scer\GAL4^ple.PF

(Inoshita et al., 2017)

abnormal neurophysiology | third instar larval stage, with Scer\GAL4^Toll-6-D42

(Inoshita et al., 2017)

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

partially lethal - majority die, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

The expression of Vps35^GD11710 under the control of either Scer\GAL4^Toll-6-D42 or Scer\GAL4^Mhc.PK leads to significant increases both in the number of type Ib synaptic boutons and in the miniature excitatory junction potential amplitude at the third instar larval neuromuscular junction, as compared to controls. Expression under the control of Scer\GAL4^ple.PF leads to a significant decrease in spontaneous synaptic activity in adult dendritic arborizing neurons and to defective sleep behavior, shown by reciprocal significant increase in sleep bouts during the light period and significant decrease in sleep bouts during the dark period, as compared to controls.

(Inoshita et al., 2017)

Expression of Vps35^GD11710 under the control of Scer\GAL4^GMR.PS does not result in any obvious eye phenotype, but does lead to a progressive defect in climbing ability, as compared to controls.

(Miura et al., 2014)

Adults expressing Vps35^GD11710 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) can show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies, depending on the particular P{GD11710} insertion line used.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 may result in semi-lethality, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 0% or 10-20% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

11710

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PS, Vps35^GD11710 has abnormal locomotor behavior | adult stage | progressive phenotype, enhanceable by Hsap\SNCA^UAS.cFa, Scer\GAL4^GMR.PS

(Miura et al., 2014)

Enhancer of

Statement

Reference

Vps35^GD11710, Scer\GAL4^GMR.PS is an enhancer of abnormal locomotor behavior | adult stage | progressive phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^GMR.PS

(Miura et al., 2014)

NOT Enhancer of

Statement

Reference

Vps35^GD11710, Scer\GAL4^ple.PU is a non-enhancer of abnormal flight | adult stage phenotype of Hsap\TMEM230^{184PGext5.UAS.2}, Scer\GAL4^ple.PU

(Ma et al., 2022)

Vps35^GD11710, Scer\GAL4^ple.PU is a non-enhancer of abnormal locomotor behavior | adult stage phenotype of Hsap\TMEM230^{184PGext5.UAS.2}, Scer\GAL4^ple.PU

(Ma et al., 2022)

Suppressor of

Statement

Reference

Vps35^GD11710, Scer\GAL4^ple.PU is a suppressor of short lived phenotype of Hsap\TMEM230^{184PGext5.UAS.2}, Scer\GAL4^ple.PU

(Ma et al., 2022)

Vps35^GD11710, Scer\GAL4^elav.PU is a suppressor of abnormal locomotor behavior | adult stage | progressive phenotype of Hsap\HTT^{128Q.1-336.UAS}, Scer\GAL4^elav.PU

(Shirasaki et al., 2012)

NOT Suppressor of

Statement

Reference

Vps35^GD11710, Scer\GAL4^ple.PU is a non-suppressor of abnormal flight | adult stage phenotype of Hsap\TMEM230^{184PGext5.UAS.2}, Scer\GAL4^ple.PU

(Ma et al., 2022)

Vps35^GD11710, Scer\GAL4^ple.PU is a non-suppressor of abnormal locomotor behavior | adult stage phenotype of Hsap\TMEM230^{184PGext5.UAS.2}, Scer\GAL4^ple.PU

(Ma et al., 2022)

Other

Statement

Reference

Hsap\SNCA^UAS.cFa, Scer\GAL4^GMR.PS, Vps35^GD11710 has visible | adult stage phenotype

(Miura et al., 2014)

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

Vps35^GD11710/Scer\GAL4^Mef2.PR is a non-suppressor of endoplasmic reticulum | larval stage phenotype of Pink1^B9

(Ham et al., 2023)

Vps35^GD11710/Scer\GAL4^Mef2.PR is a non-suppressor of embryonic/larval muscle cell | larval stage phenotype of Pink1^B9

(Ham et al., 2023)

Vps35^GD11710/Scer\GAL4^Mef2.PR is a non-suppressor of mitochondrion | adult stage phenotype of Pink1^B9

(Ham et al., 2021)

Vps35^GD11710/Scer\GAL4^Mef2.PR is a non-suppressor of flight muscle cell | adult stage phenotype of Pink1^B9

(Ham et al., 2021)

Other

Statement

Reference

Hsap\SNCA^UAS.cFa, Scer\GAL4^GMR.PS, Vps35^GD11710 has ommatidium phenotype

(Miura et al., 2014)

Hsap\SNCA^UAS.cFa, Scer\GAL4^GMR.PS, Vps35^GD11710 has interommatidial bristle phenotype

(Miura et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of Vps35^GD11710 and Hsap\SNCA^Scer\UAS.cFa under the control of Scer\GAL4^GMR.PS leads to a small reduction in size of each ommatidium along with loss of interommatidial bristles, as compared to controls; and leads to a more severe progressive climbing defect, as compared to expression of either single allele alone.

(Miura et al., 2014)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v22180

w¹¹¹⁸ P{GD11710}v22180

v45570

w¹¹¹⁸; P{GD11710}v45570