UASt regulatory sequences drive expression of an inverted repeat.
Expression of incGD7621 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to enhance RNAi effects) results in sleep defects: the flies show a reduction in sleep duration compared to controls, with a reduction in the average sleep bout length.
Both females and males expressing incGD7621 under the control of Scer\GAL4elav-C155 show a severe reduction in total sleep per day compared to controls.
Expression of incGD7621 under the control of Scer\GAL4elav-C155 does not result in a decrease in longevity.
Adults expressing incGD7621 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Expression under the control of Scer\GAL4pnr-MD237 results in bristle morphology defects on the notum in 10-20% or 30-40% of the Scer\GAL4pnr-MD237 expression domain, depending on the insertion line used.
incGD7621 is rescued by incUAS.cSa/Scer\GAL4elav-C155
Co-expression of incScer\UAS.cSa rescues the reduction in sleep/day that is seen in animals expressing incGD7621 under the control of Scer\GAL4elav-C155.