FlyBase Allele Report: Dmel\Syx1A[UAS.cBa]

General Information

Symbol

Dmel\Syx1A^UAS.cBa

Species

D. melanogaster

Name

FlyBase ID

FBal0197820

Feature type

allele

Associated gene

Dmel\Syx1A

Associated Insertion(s)

Carried in Construct

P{UAS-Syx1A.B}

Also Known As

UAS-Syx

Key Links

Transgenic product class

wild_type

(Burgess et al., 1997)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Burgess et al., 1997)

Mutagen

in vitro construct

(Saitoe et al., 2001, Burgess et al., 1997)

Progenitor genotype

Carried in construct

P{UAS-Syx1A.B}

Cytology

Description

Syx1A is expressed under the control of UAS regulatory sequences.

(Burgess et al., 1997)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Syx1A

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates amyotrophic lateral sclerosis

modeled by TBPH^Δ23

(Romano et al., 2014)

modeled by TBPH^GD6943

(Romano et al., 2014)

exacerbates neuronal ceroid lipofuscinosis 4

modeled by Hsap\DNAJC5^L116Δ.UAS

(Imler et al., 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology | heat sensitive, with Scer\GAL4^elav-C155, Syx1A^Δ229/Syx1A^3-69

(Lagow et al., 2007)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Hiesinger et al., 2005)

ommatidium, with Scer\GAL4^GMR.PF

(Hiesinger et al., 2005)

Detailed Description

Statement

Reference

Expression of Syx1A^Scer\UAS.cBa under the control of Scer\GAL4^elav-C155 has no significant effect on either the constitutive secretion rate or the amplitude of evoked EPSPs, compared to those in wild-type larvae. However, neuronal expression of Syx1A^Scer\UAS.cBa under the control of Scer\GAL4^elav-C155 in Syx1A^3-69/Syx1A^Δ229 results in a dramatic reduction in the frequency of constitutive secretion to 8.8Hz from 24.9Hz. The average amplitude of EPSPs is also significantly reduced in Syx1A^3-69/Syx1A^Δ229 mutants expressing Syx1A^Scer\UAS.cBa under the control of Scer\GAL4^elav-C155. This EPSP amplitude is similar to that in Syx1A^Scer\UAS.cBa/Scer\GAL4^elav-C155 flies, but significantly higher than wild-type.

(Lagow et al., 2007)

Syx1A^Scer\UAS.cBa; Scer\GAL4^GMR.PF flies raised at 18^oC have rough eyes.

(Hiesinger et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^elav.PU/Syx1A^UAS.cBa is a suppressor of abnormal locomotor behavior phenotype of TBPH^Δ23

(Romano et al., 2014)

Scer\GAL4^elav.PU/Syx1A^UAS.cBa is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of TBPH^Δ23

(Romano et al., 2014)

Scer\GAL4^Tub.PU/Syx1A^UAS.cBa is a suppressor of abnormal locomotor behavior | adult stage | progressive phenotype of Scer\GAL4^Tub.PU, TBPH^Δ23, TBPH^GD6943

(Romano et al., 2014)

NOT Suppressor of

Statement

Reference

Scer\GAL4^elav.PU/Syx1A^UAS.cBa is a non-suppressor of lethal - all die before end of pupal stage phenotype of TBPH^Δ23

(Romano et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

Syx1A^UAS.cBa has ommatidium phenotype, suppressible by Vha100-1¹/Scer\GAL4^GMR.PF

(Hiesinger et al., 2005)

Syx1A^UAS.cBa has eye phenotype, suppressible by Scer\GAL4^GMR.PF/Vha100-1⁴

(Hiesinger et al., 2005)

Syx1A^UAS.cBa has ommatidium phenotype, suppressible by Scer\GAL4^GMR.PF/Vha100-1⁴

(Hiesinger et al., 2005)

Syx1A^UAS.cBa has eye phenotype, suppressible by Vha100-1¹/Scer\GAL4^GMR.PF

(Hiesinger et al., 2005)

NOT suppressed by

Statement

Reference

Syx1A^UAS.cBa has eye phenotype, non-suppressible by Scer\GAL4^GMR.PF/Vha100-1²

(Hiesinger et al., 2005)

Syx1A^UAS.cBa has ommatidium phenotype, non-suppressible by Scer\GAL4^GMR.PF/Vha100-1²

(Hiesinger et al., 2005)

Syx1A^UAS.cBa has eye phenotype, non-suppressible by Vha100-1³/Scer\GAL4^GMR.PF

(Hiesinger et al., 2005)

Syx1A^UAS.cBa has ommatidium phenotype, non-suppressible by Vha100-1³/Scer\GAL4^GMR.PF

(Hiesinger et al., 2005)

Suppressor of

Statement

Reference

Scer\GAL4^elav.PU/Syx1A^UAS.cBa is a suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of TBPH^Δ23

(Romano et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Syx1A^Scer\UAS.cBa under the control of Scer\GAL4^elav.PU rescues the locomotive defects seen in TBPH^Δ23 mutant third instar larvae. The synaptic defects are also rescued. However, the expression of Syx1A^Scer\UAS.cBa under the control of Scer\GAL4^elav.PU is not sufficient to rescue the pupal lethality associated with TBPH^Δ23.

Expression of Syx1A^Scer\UAS.cBa significantly suppresses the climbing defects seen in adult hypomorphic TBPH mutants (generated by expressing TBPH^GD6943 in adult flies under the control of Scer\GAL4^tub.PU and Scer\GAL80^ts.αTub84B, in a TBPH^Δ23/+ background).

(Romano et al., 2014)

The rough eye phenotype of Syx1A^Scer\UAS.cBa; Scer\GAL4^GMR.PF flies raised at 18^oC is largely suppressed when the eye is made homozygous for Vha100-1¹ or Vha100-1⁴ but not Vha100-1² or Vha100-1³.

(Hiesinger et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Syx1A^UAS.cBa/Scer\GAL4^elav.PLu rescues Syx1A^L371

(Saitoe et al., 2001)

Syx1A^UAS.cBa/Scer\GAL4^hs.PB rescues Syx1A^L371

(Burgess et al., 1997)

Partially rescues

Scer\GAL4^elav-C155/Syx1A^UAS.cBa partially rescues Syx1A^Δ229/Syx1A^3-69

(Lagow et al., 2007)

Fails to rescue

Scer\GAL4^G14/Syx1A^UAS.cBa fails to rescue Syx1A^L371

(Saitoe et al., 2001)

Comments

Evoked and mESCs are readily observed in Syx1A^L371 embryos expressing Syx1A^Scer\UAS.cSa under the control of Scer\GAL4^elav.PLu, but not in Syx1A^L371 embryos expressing Syx1A^Scer\UAS.cSa under the control of Scer\GAL4^G14.

(Saitoe et al., 2001)

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

51618

P{UAS-Syx1A.B}2, y¹ w^*

51619

y¹ w^*; P{UAS-Syx1A.B}6

51620

y¹ w^*; P{UAS-Syx1A.B}13B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

Syx1A^Scer\UAS.cBa

(Schwarz, 2007.3.20)

Syx1A^Scer\UAS.cSa

Syx1A^UAS.cBa

syx1A^Scer\UAS.cBa

syx1A^UAS.cBa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Burgess

(Burgess et al., 1997)

Saccharomyces cerevisiae UAS construct a of Saitoe

(Saitoe et al., 2001)

Secondary FlyBase IDs

FBal0065668
FBal0126478

References (9)

Report Sections

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