FlyBase Allele Report: Dmel\trol[null]

General Information

Symbol

Dmel\trol^null

Species

D. melanogaster

Name

FlyBase ID

FBal0197223

Feature type

allele

Associated gene

Dmel\trol

Associated Insertion(s)

Carried in Construct

Also Known As

pcan^null

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Voigt et al., 2002)

Progenitor genotype

P{EP}EP1619

(Voigt et al., 2002)

P{lacW}trol^G0271

(Voigt et al., 2002)

Cytology

Description

Simultaneous mobilisation of the two progenitor insertions, resulting in a 47908bp deletion that spans the entire trol transcription unit.

(Voigt et al., 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

X:2,470,235..2,518,143 [-]

Comment:

A 47,908 bp deficiency resulting from the simultaneous mobilization of P{EP}EP1619 and P{lacW}trol^G0271. Site of deletion on reference sequence inferred by FlyBase curator.

(Voigt et al., 2002)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | embryonic stage

(Cho et al., 2012)

lethal

(Voigt et al., 2002)

Phenotype Manifest In

embryonic/larval hemocyte

(Nagaosa et al., 2011)

follicle cell | somatic clone

(Schneider et al., 2006)

germarium cap cell | somatic clone

(Díaz-Torres et al., 2021)

larval intersegmental nerve branch ISNb of A1-7 | embryonic stage

(Cho et al., 2012)

larval segmental nerve branch SNa of A1-7 | embryonic stage

(Cho et al., 2012)

Detailed Description

Statement

Reference

trol^null cap cell clones, induced during the larval and pupal stages, result in cap cell displacement from the niche; only germaria containing 3 or more mutant cap cells showed the cap cell displacement.

(Díaz-Torres et al., 2021)

Hemizygous embryos show ISNb motor axon guidance defects (59.9% of hemisegments) and SNa motor axon guidance defects (38.7% of hemisegments). ISNb axons fail to defasciculate at muscles 6 and 7 and some axons stall between muscles 6 and 13. SNa axons fail to reach their proper targets.

(Cho et al., 2012)

trol^null hemizygous embryonic hemocytes show decreased competence to undertake phagocytosis of apoptotic cells, as compared to controls.

(Nagaosa et al., 2011)

Homozygous follicle cell clones have normal apical-basal polarity under normal food conditions.

(Mirouse et al., 2009)

Homozygous somatic follicle-cell clones often lose epithelial tissue organisation; about 50% of clones in stage 6-9 egg chambers show a multilayer phenotype.

(Schneider et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

trol[+]/trol^null is a suppressor of abnormal neuroanatomy | embryonic stage phenotype of Scer\GAL4^P52, Sema1a^UAS.cYa, Sema1a^k13702

(Cho et al., 2012)

Other

Statement

Reference

Sema1a^k13702, trol[+]/trol^null has abnormal neuroanatomy | dominant | embryonic stage phenotype

(Cho et al., 2012)

Df(4)C3/+, trol^null has abnormal neuroanatomy | dominant | embryonic stage phenotype

(Cho et al., 2012)

Phenotype Manifest In

Suppressor of

Statement

Reference

trol[+]/trol^null is a suppressor of larval posterior commissure | embryonic stage phenotype of Scer\GAL4^P52, Sema1a^UAS.cYa, Sema1a^k13702

(Cho et al., 2012)

Other

Statement

Reference

Sema1a^k13702, trol[+]/trol^null has larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype

(Cho et al., 2012)

Sema1a^k13702, trol[+]/trol^null has larval segmental nerve branch SNa of A1-7 | embryonic stage phenotype

(Cho et al., 2012)

Df(4)C3/+, trol^null has larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype

(Cho et al., 2012)

Df(4)C3/+, trol^null has larval segmental nerve branch SNa of A1-7 | embryonic stage phenotype

(Cho et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

The commissural axon phenotype (failure to cross the midline) seen in embryos expressing Sema-1a^Scer\UAS.cYa under the control of Scer\GAL4^P52 in a Sema-1a^k13702 null background is significantly suppressed if the embryos are also heterozygous for trol^null.

trol^null/+ ; Sema-1a^k13702/+ double heterozygous embryos show ISNb motor axon guidance defects (51.0% of hemisegments) and SNa motor axon guidance defects (41.8% of hemisegments).

trol^null/+ ; Df(4)C3/+ double heterozygous embryos show ISNb motor axon guidance defects (51.5% of hemisegments) and SNa motor axon guidance defects (40% of hemisegments).

(Cho et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

trol^null is partially rescued by trol^UAS.RG/Scer\GAL4^elav.PU

(Cho et al., 2012)

Not rescued by

trol^null is not rescued by trol^UAS.RG/Scer\GAL4^twi.PU

(Cho et al., 2012)

Comments

Expression of trol^Scer\UAS.RG under the control of Scer\GAL4^elav.PU significantly rescues the ISNb and SNa motor axon guidance defects seen in trol^null embryos. Expression under the control of Scer\GAL4^repo.PU only very modestly rescues the motor axon defects, and expression under the control of Scer\GAL4^twi.PU fails to rescue the motor axon defects.

(Cho et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

99934

trol^null w^* P{FRT(w^hs)}101/FM7i, P{ActGFP}JMR3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Pcan^null

(Cho et al., 2012)

pcan^null

(Mirouse et al., 2009, Denef et al., 2008, Schneider et al., 2006)

trol^null

(Guss et al., 2023, Díaz-Torres et al., 2021, Matsubayashi et al., 2020, Kanda et al., 2019, Dai et al., 2018, Matsubayashi et al., 2017, Nagaosa et al., 2011, Schneider et al., 2006, Voigt et al., 2002)

ΔPerl

(Matsubayashi et al., 2020)

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

Open Close