FlyBase Allele Report: Dmel\upd2[Δ3-62]

General Information

Symbol

Dmel\upd2^Δ3-62

Species

D. melanogaster

Name

FlyBase ID

FBal0194746

Feature type

allele

Associated gene

Dmel\upd2

Associated Insertion(s)

Carried in Construct

Also Known As

upd2^Δ

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Hombria et al., 2005)

Progenitor genotype

upd2^GG01031

(Hombria et al., 2005)

Cytology

Description

Imprecise excision of the P{Mae-UAS.6.11} element, which results in deletion of 2530bp of the upd2 genomic region, from 2264bp upstream of the AUG codon to 266bp downstream. This removes the 5'UTR and coding sequence for the first 89 amino acid residues of the protein product.

(Hombria et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

X:18,242,739..18,245,269 [-]

Comment:

A 2530 bp deletion resulting from the imprecise excision of P{Mae-UAS.6.11}upd2^GG01031, which extends from 2264bp upstream of the upd2 AUG codon to 266bp downstream.

(Hombria et al., 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cancer

modeled by Ras85D^V12.UAS, l(2)gl^unspecified

(Wu et al., 2010)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased body size | adult stage

(Rajan and Perrimon, 2012)

decreased cell number | adult stage

(Rajan and Perrimon, 2012)

decreased cell size | adult stage

(Rajan and Perrimon, 2012)

fertile | recessive

(Hombria et al., 2005)

viable | recessive

(Hombria et al., 2005)

Phenotype Manifest In

embryonic/larval oenocyte | third instar larval stage

(Rajan and Perrimon, 2012)

wing

(Rajan and Perrimon, 2012)

Detailed Description

Statement

Reference

Hemizygous adult males appear smaller and slimmer than wild-type controls. Wing area is reduced by 10% overall and there is a significant reduction in both cell number and cell size compared to wild type.

Mutant adults show a significant reduction in triacylglycerol levels and a significant increase in circulating glucose levels compared to controls.

Mutant adults do not show defects in feeding behaviour.

Mutant larvae show a striking accumulation of lipid droplets in oenocytes under fed conditions, in contrast to wild-type controls.

(Rajan and Perrimon, 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

upd2^Δ3-62 has decreased body size | adult stage phenotype, suppressible by Scer\GAL4^Ilp3.PB/Bhal\NaChBac^UAS.EGFP

(Rajan and Perrimon, 2012)

upd2^Δ3-62 has decreased body size | adult stage phenotype, suppressible by Scer\GAL4^Cg.PA/Hsap\LEP^UAS.cRa

(Rajan and Perrimon, 2012)

NOT suppressed by

Statement

Reference

upd2^Δ3-62 has decreased body size | adult stage phenotype, non-suppressible by Scer\GAL4^Ilp3.PB/Sh^{EKO.UAS.GFP(GL)}

(Rajan and Perrimon, 2012)

Enhancer of

Statement

Reference

upd3^Δ, upd3^Δ, upd2^Δ3-62, upd2^Δ3-62 is an enhancer of visible | adult stage phenotype of Bub3^GD9924, Scer\GAL4^ey.PH

(Clemente-Ruiz et al., 2016)

upd3^Δ, upd3^Δ, upd2^Δ3-62, upd2^Δ3-62 is an enhancer of abnormal size | adult stage phenotype of Bub3^GD9924, Scer\GAL4^ey.PH

(Clemente-Ruiz et al., 2016)

upd3^Δ, upd3^Δ, upd2^Δ3-62, upd2^Δ3-62 is an enhancer of visible phenotype of Bub3^GD9924, Scer\GAL4^ey.PH

(Clemente-Ruiz et al., 2016)

Suppressor of

Statement

Reference

upd2^Δ3-62 is a suppressor of neoplasia | somatic clone | cell autonomous phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, l(2)gl^unspecified, upd1^RNAi.UAS.cUa

(Wu et al., 2010)

NOT Suppressor of

Statement

Reference

upd2^Δ3-62, Scer\GAL4^Act.PU is a non-suppressor of neoplasia | somatic clone | cell autonomous phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, l(2)gl^unspecified

(Wu et al., 2010)

Phenotype Manifest In

Suppressed by

Statement

Reference

upd2^Δ3-62 has wing phenotype, suppressible by Scer\GAL4^Ilp3.PB/Bhal\NaChBac^UAS.EGFP

(Rajan and Perrimon, 2012)

upd2^Δ3-62 has wing phenotype, suppressible by Scer\GAL4^Cg.PA/Hsap\LEP^UAS.cRa

(Rajan and Perrimon, 2012)

NOT suppressed by

Statement

Reference

upd2^Δ3-62 has wing phenotype, non-suppressible by Scer\GAL4^Ilp3.PB/Sh^{EKO.UAS.GFP(GL)}

(Rajan and Perrimon, 2012)

Enhancer of

Statement

Reference

upd3^Δ, upd3^Δ, upd2^Δ3-62, upd2^Δ3-62 is an enhancer of eye phenotype of Bub3^GD9924, Scer\GAL4^ey.PH

(Clemente-Ruiz et al., 2016)

Suppressor of

Statement

Reference

upd2^Δ3-62 is a suppressor of eye disc | somatic clone | cell autonomous phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, l(2)gl^unspecified, upd1^RNAi.UAS.cUa

(Wu et al., 2010)

NOT Suppressor of

Statement

Reference

upd2^Δ3-62, Scer\GAL4^Act.PU is a non-suppressor of eye disc | somatic clone | cell autonomous phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, l(2)gl^unspecified

(Wu et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of upd1^{dsRNA.Scer\UAS.cUa} partially suppresses the overgrowth and invasion of the nerve cord seen in l(2)gl^unspecified mutant eye-antennal disc clones that simultaneously express Ras85D^V12.Scer\UAS under the control of Scer\GAL4^Act.PU. The phenotype is further suppressed when the clones are generated in a upd2^Δ3-62 mutant background.

upd2^Δ3-62 does not suppress the overgrowth and invasion of the nerve cord seen in l(2)gl^unspecified mutant eye-antennal disc clones that simultaneously express Ras85D^V12.Scer\UAS under the control of Scer\GAL4^Act.PU.

(Wu et al., 2010)

Xenogenetic Interactions

Statement

Reference

Expression of Bhal\NaChBac^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^Ilp3.PB rescues the reduced wing area of upd2^Δ3-62 mutants.

Expression of Sh^{EKO.Scer\UAS.T:Avic\GFP-GL} under the control of Scer\GAL4^Ilp3.PB does exacerbate the reduced wing area phenotype of upd2^Δ3-62 mutants.

Expression of Hsap\LEP^Scer\UAS.cRa under the control of Scer\GAL4^Cg.PA rescues the reduced wing area and reduced triacylglycerol levels seen in upd2^Δ3-62 flies.

(Rajan and Perrimon, 2012)

Complementation and Rescue Data

Rescued by

upd2^Δ3-62 is rescued by upd2^UAS.EGFP/Scer\GAL4^Cg.PA

(Rajan and Perrimon, 2012)

Comments

The reduction in wing size and reduction in triacylglycerol levels seen in upd2^Δ3-62 flies is rescued by expression of upd2^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^Cg.PA.

(Rajan and Perrimon, 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

upd2^3-62

(Wu et al., 2010)

upd2^Δ3-62

(Dong et al., 2021, Brent and Rajan, 2020, Hombria et al., 2005)

upd2^Δ

(Dong et al., 2021, Gao et al., 2015, Rajan and Perrimon, 2012)

upd2del362

(Wilson et al., 2020)

upd2Δ

(Clemente-Ruiz et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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