FlyBase Allele Report: Dmel\upd2[UAS.EGFP]

General Information

Symbol

Dmel\upd2^UAS.EGFP

Species

D. melanogaster

Name

FlyBase ID

FBal0194744

Feature type

allele

Associated gene

Dmel\upd2

Associated Insertion(s)

Carried in Construct

P{UAS-upd2.EGFP}

Also Known As

UAS-Upd2

Key Links

Transgenic product class

wild_type

(Hombria et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Hombria et al., 2005)

Mutagen

in vitro construct

(Hombria et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-upd2.EGFP}

Cytology

Description

UASt regulatory sequences drive expression of the 1.8kb open reading frame of upd2, which is tagged with EGFP.

(Hombria et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

upd2

Tagged with

EGFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal starvation stress response | adult stage, with Scer\GAL4^ppl.PP

(Pathak and Varghese, 2021)

short lived | nutrition conditional, with Scer\GAL4^Hml.Δ

(Woodcock et al., 2015)

Phenotype Manifest In

wing, with Scer\GAL4^Bx-MS1096

(Hombria et al., 2005)

Detailed Description

Statement

Reference

Adults expressing upd2^UAS.EGFP under the control of Scer\GAL4^ppl.PP are resistant to starvation.

(Pathak and Varghese, 2021)

Expression of upd2^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^Hml.Δ impairs the lifespan of adult flies on a standard diet but does not further decrease the lowered survival of flies fed lard-supplemented diet.

(Woodcock et al., 2015)

Expression of upd2^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^ppl.PP suppresses the reduction in triacylglycerol levels that normally occurs under starvation conditions.

(Rajan and Perrimon, 2012)

Expression of upd2^{Scer\UAS.T:Avic\GFP-EGFP} in wing imaginal discs, driven by Scer\GAL4^Bx-MS1096, results in adults with wings of reduced size.

(Hombria et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

upd2^UAS.EGFP, Scer\GAL4^ppl.PP is a suppressor | partially of abnormal starvation stress response | adult stage phenotype of Edem1^GD3196, Scer\GAL4^ppl.PP

(Pathak and Varghese, 2021)

Scer\GAL4^elav.PU/upd2^UAS.EGFP is a suppressor of abnormal feeding behavior | adult stage phenotype of Scer\GAL4^elav.PU, upd1^JF03149

(Beshel et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^Bx-MS1096, upd2^UAS.EGFP has wing phenotype, suppressible by dome^ΔEXT.UAS/Scer\GAL4^Bx-MS1096

(Hombria et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

The increased attraction to food cues, increased food consumption and higher body weight of adult flies expressing upd1^JF03149 under the control of Scer\GAL4^elav.PU can be rescued by co-expression of upd2^{Scer\UAS.T:Avic\GFP-EGFP}.

(Beshel et al., 2017)

Coexpression of the dome^{ΔEXT.Scer\UAS} transgene with upd2^{Scer\UAS.T:Avic\GFP-EGFP} (both under the control of Scer\GAL4^Bx-MS1096) suppresses the reduced wing phenotype seen when upd2^{Scer\UAS.T:Avic\GFP-EGFP} is expressed alone.

Expression of upd2^{Scer\UAS.T:Avic\GFP-EGFP} in the spiracles, driven by Scer\GAL4^klu-G410, results in partial rescue of the Df(1)os1A2 spiracle defect.

(Hombria et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

upd2^UAS.EGFP/Scer\GAL4^Cg.PA rescues upd2^Δ3-62

(Rajan and Perrimon, 2012)

Comments

The reduction in wing size and reduction in triacylglycerol levels seen in upd2^Δ3-62 flies is rescued by expression of upd2^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^Cg.PA.

(Rajan and Perrimon, 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

upd2^{Scer\UAS.T:Avic\GFP-EGFP}

upd2^UAS.EGFP

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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