FlyBase Allele Report: Dmel\upd1[JF03149]

General Information

Symbol

Dmel\upd1^JF03149

Species

D. melanogaster

Name

FlyBase ID

FBal0239648

Feature type

allele

Associated gene

Dmel\upd1

Associated Insertion(s)

Carried in Construct

P{TRiP.JF03149}

Also Known As

UAS-upd1^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

(Perkins et al., 2009)

Progenitor genotype

Carried in construct

P{TRiP.JF03149}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-JF03149

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of congenital leptin deficiency

CEA

(Beshel et al., 2017)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal feeding behavior | adult stage, with Scer\GAL4^elav.PU

(Beshel et al., 2017)

abnormal size, with Scer\GAL4^en-e16E

(Djiane et al., 2013)

increased cell number | larval stage, with Scer\GAL4^Hand.Δ

(Destalminil-Letourneau et al., 2021)

increased cell number | larval stage, with Scer\GAL4^NP1029

(Destalminil-Letourneau et al., 2021)

Phenotype Manifest In

embryonic/larval crystal cell | increased number | larval stage, with Scer\GAL4^Hand.Δ

(Destalminil-Letourneau et al., 2021)

embryonic/larval crystal cell | increased number | larval stage, with Scer\GAL4^NP1029

(Destalminil-Letourneau et al., 2021)

wing | posterior, with Scer\GAL4^en-e16E

(Djiane et al., 2013)

Detailed Description

Statement

Reference

Expression of upd1^JF03149 under the control of Scer\GAL4^Cg.PA has no effect on either body weight or attraction to food cues in adult flies, whereas expression driven in the neural tissue by Scer\GAL4^elav.PU heightens the attraction to food in fed flies so that it is comparable to the response observed in their starved counterparts as well as starved control flies (and higher than in fed controls). Scer\GAL4^elav.PU>upd1^JF03149 flies also consume more food and weigh more than controls (but their relative fat storage levels are not significantly increased).

Limiting the Scer\GAL4^elav.PU-driven expression of upd1^JF03149 only to the developmental stage (by using tub-Gal80[ts] in conjunction with temperature manipulations to control the temporal expression profile) does not lead to any significant changes in response to food odor, food intake or average body weight in adult flies, whereas adult-onset upd1 knock-down results in a marked increase in all three, as compared to controls.

(Beshel et al., 2017)

Scer\GAL4^en-e16E-mediated expression results in a modest reduction in size of the posterior compartment of the adult wing.

(Djiane et al., 2013)

Expression of upd1^JF03149 under the control of Scer\GAL4^ppl.PP does not result in a reduction in body size.

Expression of upd1^JF03149 under the control of Scer\GAL4^Cg.PA has no effect on triacylglycerol levels in adults.

(Rajan and Perrimon, 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^elav.PU, upd1^JF03149 has abnormal feeding behavior | adult stage phenotype, suppressible by Scer\GAL4^elav.PU/upd2^UAS.EGFP

(Beshel et al., 2017)

Scer\GAL4^elav.PU, upd1^JF03149 has abnormal feeding behavior | adult stage phenotype, suppressible by Hsap\LEP^UAS.cRa, Scer\GAL4^elav.PU

(Beshel et al., 2017)

Suppressor of

Statement

Reference

upd1^JF03149/upd3^HM05061, Scer\GAL4^ap-md544 is a suppressor of abnormal developmental rate phenotype of BacA\p35^UAS.cHa, Bub3^GD9924, Scer\GAL4^ap-md544

(Romão et al., 2021)

upd1^JF03149, Scer\GAL4^ptc-559.1 is a suppressor | partially of hyperplasia phenotype of N^UAS.icd, Scer\GAL4^ptc-559.1

(Djiane et al., 2013)

NOT Suppressor of

Statement

Reference

upd1^JF03149, Scer\GAL4^ap-md544 is a non-suppressor of abnormal developmental rate phenotype of BacA\p35^UAS.cHa, Bub3^GD9924, Scer\GAL4^ap-md544

(Romão et al., 2021)

upd1^JF03149/Ilp8^GD1670, Scer\GAL4^ap-md544 is a non-suppressor of abnormal developmental rate phenotype of BacA\p35^UAS.cHa, Bub3^GD9924, Scer\GAL4^ap-md544

(Romão et al., 2021)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The increased attraction to food cues, increased food consumption and higher body weight of adult flies expressing upd1^JF03149 under the control of Scer\GAL4^elav.PU can be rescued by co-expression of upd2^{Scer\UAS.T:Avic\GFP-EGFP}.

(Beshel et al., 2017)

Xenogenetic Interactions

Statement

Reference

(Beshel et al., 2017)

Complementation and Rescue Data

Rescued by

upd1^JF03149 is rescued by Scer\GAL4^elav.PU/upd1^UAS.cUa

(Beshel et al., 2017)

Comments

(Beshel et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

28722

y¹ v¹; P{TRiP.JF03149}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Upd1^RNAi

(Ma et al., 2019)

os^JF03149

upd1^JF03149

Name Synonyms

Secondary FlyBase IDs

References (13)

Report Sections

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