FlyBase Allele Report: Dmel\ft[ΔICD.UAS.Tag:HA]

General Information

Symbol

Dmel\ft^{ΔICD.UAS.Tag:HA}

Species

D. melanogaster

Name

FlyBase ID

FBal0193592

Feature type

allele

Associated gene

Dmel\ft

Associated Insertion(s)

Carried in Construct

P{UAS-ft.ΔICD.HA}

Also Known As

UAS-ftΔICD, UAS-ft^ΔICD

Key Links

Transgenic product class

dominant_negative_variant

(Matakatsu and Blair, 2006)

inframe_deletion

(Matakatsu and Blair, 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Matakatsu and Blair, 2006)

inframe_deletion

(Matakatsu and Blair, 2006)

Mutagen

in vitro construct

(Matakatsu and Blair, 2006)

Progenitor genotype

ft^UAS.cMa

(Matakatsu and Blair, 2006)

Carried in construct

P{UAS-ft.ΔICD.HA}

Cytology

Description

Nucleotide 13858 of the ft coding region to the stop codon has been replaced with a Tag:HA tag.

(Matakatsu and Blair, 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

wing, with Scer\GAL4^Act5C.PI

(Matakatsu and Blair, 2006)

wing disc, with Scer\GAL4^Act5C.PI, ft⁸/ft^G-rv

(Matakatsu and Blair, 2006)

wing disc, with Scer\GAL4^da.G32, ft⁸/ft^G-rv

(Matakatsu and Blair, 2006)

wing disc, with Scer\GAL4^hh.PU

(Degoutin et al., 2013)

wing disc, with Scer\GAL4^hh-Gal4

(Matakatsu and Blair, 2012, Willecke et al., 2006)

wing disc | dorsal, with Scer\GAL4^ap-md544

(Matakatsu and Blair, 2006)

wing disc | posterior, with Scer\GAL4^en-e16E

(Matakatsu and Blair, 2006)

wing disc | posterior, with Scer\GAL4^hh-Gal4

(Matakatsu and Blair, 2006)

Detailed Description

Statement

Reference

Expression of ft^{ΔICD.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^ptc.PU in cells of the wing disc proper has no significant effect on the morphology or proliferation of the overlying peripodial membrane cells in third instar larvae.

(Panneton et al., 2015)

Expression of ft^{ΔICD.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^hh.PU causes substantial overgrowth and folding of the posterior wing disc.

(Degoutin et al., 2013)

Expression of ft^{ΔICD.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^hh-Gal4 induces massive overgrowth of the wing disc.

(Matakatsu and Blair, 2012)

Expression of ft^{ΔICD.Scer\UAS.T:Ivir\HA1} in dorsal cells of the wing disc, driven by Scer\GAL4^ap-md544, results in the autonomous overgrowth of this region. Likewise, expression in the posterior, driven by Scer\GAL4^en-e16E or Scer\GAL4^hh-Gal4, causes autonomous overgrowth of the posterior region.

Expression of ft^{ΔICD.Scer\UAS.T:Ivir\HA1}, under the control of either Scer\GAL4^Act5C.PI or Scer\GAL4^da.G32, enhances the wing disc tissue overgrowth phenotype of ft^G-rv/ft⁸ mutants.

Expression of ft^{ΔICD.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Act5C.PI causes mild perturbation of planar cell polarity in the wing and a foreshortening along the proximodistal axis of the wing blade as the anterior and posterior crossveins are abnormally close.

(Matakatsu and Blair, 2006)

Expression of ft^{ΔICD.Scer\UAS.T:Ivir\HA1} in the wing disc, under the control of Scer\GAL4^hh-Gal4 results in an overgrowth of the posterior compartment.

(Willecke et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^hh.PU, ft^{ΔICD.UAS.Tag:HA} has increased cell number phenotype, suppressible | partially by wap^VDRC.cUa/Scer\GAL4^hh.PU

(Degoutin et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^hh.PU, ft^{ΔICD.UAS.Tag:HA} has wing disc phenotype, suppressible | partially by wap^VDRC.cUa/Scer\GAL4^hh.PU

(Degoutin et al., 2013)

Scer\GAL4^hh-Gal4, ft^{ΔICD.UAS.Tag:HA} has wing disc phenotype, suppressible | partially by ds^UAO71

(Matakatsu and Blair, 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of wap^VDRC.cUa partially suppresses the wing disc overgrowth phenotype seen when ft^{ΔICD.Scer\UAS.T:Ivir\HA1} is expressed under the control of Scer\GAL4^hh.PU.

(Degoutin et al., 2013)

The overgrowth of the wing disc caused by expression of ft^{ΔICD.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^hh-Gal4 is partially suppressed in a ds^UAO71 background.

(Matakatsu and Blair, 2012)

S2 cells that express ft^{ΔICD.Scer\UAS.T:Ivir\HA1} and ds^Scer\UAS.cTa, under the control of Scer\GAL4^Act5C.PI, aggregate.

(Matakatsu and Blair, 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Scer\GAL4^Act.PU/ft^{ΔICD.UAS.Tag:HA} partially rescues ft⁸/ft^G-rv

(Matakatsu and Blair, 2012)

Fails to rescue

Scer\GAL4^hh-Gal4/ft^{ΔICD.UAS.Tag:HA} fails to rescue ft^unspecified

(Matakatsu and Blair, 2012)

Scer\GAL4^Act5C.PI/ft^{ΔICD.UAS.Tag:HA} fails to rescue ft⁸/ft^G-rv

(Matakatsu and Blair, 2006)

Scer\GAL4^da.G32/ft^{ΔICD.UAS.Tag:HA} fails to rescue ft⁸/ft^G-rv

(Matakatsu and Blair, 2006)

Scer\GAL4^en-e16E/ft^{ΔICD.UAS.Tag:HA} fails to rescue ft⁸/ft^G-rv

(Matakatsu and Blair, 2006)

Comments

Expression of ft^{ΔICD.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Act.PU in ft^G-rv/ft⁸ animals does not rescue eclosion but very weakly improves hair planar cell polarity defects in the abdomen of pharate adults.

Expression of ft^{ΔICD.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^hh-Gal4 fails to rescue overgrowth of the posterior compartment of the wing disc in ft^unspecified animals.

(Matakatsu and Blair, 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Carried in a plasmid and transfected into S2 cells for use in cell aggregation assays.

(Matakatsu and Blair, 2006)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

ft^{ΔICD.Scer\UAS.T:Ivir\HA1}

ft^{ΔICD.UAS.Tag:HA}

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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