FB2024_03 , released June 25, 2024
Allele: Dmel\DarkD1292A.UAS.Tag:polyHis,Tag:MYC
Open Close
General Information
Symbol
Dmel\DarkD1292A.UAS.Tag:polyHis,Tag:MYC
Species
D. melanogaster
Name
FlyBase ID
FBal0193515
Feature type
allele
Associated gene
Dmel\Dark
Associated Insertion(s)
Carried in Construct
P{UAS-Dark.D1292A}
Also Known As
UAS-darkV
Key Links
Transgenic product class
gain_of_function_variant
(Akdemir et al., 2006)
missense_variant
(Akdemir et al., 2006)
Mutagen
Nature of the Allele
Transgenic product class
gain_of_function_variant
(Akdemir et al., 2006)
missense_variant
(Akdemir et al., 2006)
Mutagen
in vitro construct
(Akdemir et al., 2006)
Progenitor genotype
Carried in construct
P{UAS-Dark.D1292A}
Cytology
Description

UASt regulatory sequences drive expression of Dark coding sequences, with a mutated caspase site (carries the amino acid replacement D1292A), with 8xTag:polyHis at the N-terminal end and 6xTag:MYC tags at the carboxy terminal end.

(Akdemir et al., 2006)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Dark
Tagged with
Tag:MYC
Tag:polyHis
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of ArkScer\UAS.T:Zzzz\His6,T:Hsap\MYC in hemocytes, under the control of Scer\GAL4Hml.PG almost completely restores apoptosis levels after Smac mimetic treatment.

      (Akdemir et al., 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      DarkD1292A.UAS.Tag:polyHis,Tag:MYC/Scer\GAL4Tub.PU rescues Dark82

      (Akdemir et al., 2006)

      DarkD1292A.UAS.Tag:polyHis,Tag:MYC/Scer\GAL4da.G32 rescues Dark82

      (Akdemir et al., 2006)

      Scer\GAL4Hml.PG/DarkD1292A.UAS.Tag:polyHis,Tag:MYC rescues Dark82

      (Akdemir et al., 2006)
      Partially rescues

      DarkD1292A.UAS.Tag:polyHis,Tag:MYC/Scer\GAL4C833 partially rescues Dark82

      (Akdemir et al., 2006)

      DarkD1292A.UAS.Tag:polyHis,Tag:MYC partially rescues Dark82

      (Akdemir et al., 2006)
      Fails to rescue

      Scer\GAL4c81/DarkD1292A.UAS.Tag:polyHis,Tag:MYC fails to rescue Dark82

      (Akdemir et al., 2006)
      Comments

      Adult Ark82 flies rescued to viability by expression of ArkB1292A.Scer\UAS.T:Zzzz\His6,T:Hsap\MYC under the control of Scer\GAL4unspecified display split thorax phenotypes and bristle abnormalities in the notum.

      Surprisingly, in the absence of any Scer\GAL4 driver, `leaky' expression of ArkB1292A.Scer\UAS.T:Zzzz\His6,T:Hsap\MYC partially rescues Ark82 lethality.

      (Akdemir et al., 2006)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      ArkB1292A.Scer\UAS.T:Zzzz\His6,T:Hsap\MYC
      DarkB1292A.Scer\UAS.T:Zzzz\His6,T:Hsap\MYC
      DarkD1292A.UAS.Tag:polyHis,Tag:MYC
      Name Synonyms
      Secondary FlyBase IDs
        References (3)