Expression of cidScer\UAS.cJa under the control of Scer\GAL4GMR.PF results in a rough eye phenotype.
Expression of cidScer\UAS.cJa under the control of Scer\GAL4ey.PH results in a severe rough eye phenotype.
Expression of cidScer\UAS.cJa under the control of Scer\GAL4GMR.PF results in a rough eye phenotype, primarily in the posterior part of the eye.
Scer\GAL4GMR.PF, cidUAS.cJa has visible phenotype, enhanceable by Cenp-C[+]/Cenp-CIR35
Scer\GAL4GMR.PF, cidUAS.cJa has visible phenotype, enhanceable by Cap-G49Ff-3/l(2)49Ff[+]
Scer\GAL4GMR.PF, cidUAS.cJa has visible phenotype, enhanceable by l(2)49Ff[+]/Cap-G49Ff-6
Scer\GAL4GMR.PF, cidUAS.cJa has visible phenotype, enhanceable by Df(2R)CX1/+
Scer\GAL4GMR.PF, cidUAS.cJa has visible phenotype, enhanceable by Df(2R)vg56/+
Scer\GAL4GMR.PF, cidUAS.cJa has visible phenotype, enhanceable by l(2)49Ff[+]/Cap-G49Ff-1
Scer\GAL4GMR.PF, cidUAS.cJa has visible phenotype, non-enhanceable by Df(2R)vg-D/+
Scer\GAL4GMR.PF, cidUAS.cJa has eye phenotype, enhanceable by Cenp-C[+]/Cenp-CIR35
Scer\GAL4GMR.PF, cidUAS.cJa has eye phenotype, enhanceable by Cap-G49Ff-3/l(2)49Ff[+]
Scer\GAL4GMR.PF, cidUAS.cJa has eye phenotype, enhanceable by l(2)49Ff[+]/Cap-G49Ff-6
Scer\GAL4GMR.PF, cidUAS.cJa has eye phenotype, enhanceable by Df(2R)CX1/+
Scer\GAL4GMR.PF, cidUAS.cJa has eye phenotype, enhanceable by Df(2R)vg56/+
Scer\GAL4GMR.PF, cidUAS.cJa has eye phenotype, enhanceable by l(2)49Ff[+]/Cap-G49Ff-1
Scer\GAL4GMR.PF, cidUAS.cJa has eye phenotype, non-enhanceable by Df(2R)vg-D/+
Simultaneous co-expression of cal1Scer\UAS.T:Avic\GFP-EGFP and cidScer\UAS.cJa under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 results in mitotic defects in embryos at mitosis 16.
Simultaneous co-expression of cal1Scer\UAS.T:Avic\GFP-EGFP, cidScer\UAS.cJa and Cenp-CScer\UAS.cHa under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 results in strong mitotic defects in embryos at mitosis 16. The most prominent defects are abnormal anaphase and telophase figures with chromatin bridges containing lagging centromeres.
Embryos simultaneously co-expressing cal1Scer\UAS.cSa, cidScer\UAS.cJa and Cenp-CScer\UAS.cHa under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 already show mitotic defects during mitosis 15. Chromosome congression into a metaphase plate is always slower than normal and metaphase is usually prolonged. Chromosome segregation is abnormal in approximately half of anaphases, with the defect usually consisting of lagging centromeres. The distance between sister kinetochores in metaphase plates is scattered over a larger range in the mutant embryos compared to the range seen in controls.