Apparently normal ciliary rootlets form in the olfactory neurons of mutant adults. Mutant adults show normal climbing ability in a negative geotaxis assay.

Cep135^c04199/Df(3L)Brd15 mutant neuroblasts show symmetric centrosome behavior at interphase: in wild type one apical microtubule organizing center (MTOC) is seen whereas in Cep135^c04199/Df(3L)Brd15 two centromeres of similar size and MTOC activity are observed close together on the apical cortex. The two centrosomes progressively separate from each other until they reach their respective positions on the apical and basal cortex by propmetaphase. Rate and direction of centriole migration appears normal. Unlike in wild type, the pericentriolar matrix (PCM) is not downregulated after centrosome splitting (the 'shedding phase'). Moreover shedding phase basal centrioles fail to undergo the reduction in size seen in wild type, with the basal centrioles appearing similar in size to wild type apical centrosomes. As in wild type, the Cnb+ mother centriole is larger in Cep135^c04199/Df(3L)Brd15 mutants, but 45% of neuroblasts wrongly retain the Cnb[+] centriole and segregate the daughter centriole into the ganglion mother cell.

Neuroblast numbers are unchanged in Cep135^c04199 mutant brains compared to control brains and no symmetric neuroblast divisions are observed. Mutant centrosomes prematurely form misaligned bipolar spindles, but spindle rotation during metaphase corrects this misalignment.

Only 14% of basal body/ciliary structures of mutant G2 spermatocytes contain a "singlet microtubule" (a microtubule within the basal body lumen that in some cases extends uninterruptedly into the cilium), in contrast to 48% of basal body/ciliary complexes wild-type G2 spermatocytes. The majority of basal bodies of mutant early spermatids do not contain a singlet microtubule (in contrast wild type, where 80% of early spermatid basal bodies contain one).

Cep135^c04199 mutant mature primary spermatocytes exhibit clear central cartwheel defects. Mutant centrioles do not contain a detectable central tube. Nevertheless, when viewed in cross section, the structure and ninefold symmetry of the centriolar microtubule blades appears unperturbed.

Cep135[c04199] cartwheel defects are milder in younger centrioles. Relatively normal cartwheels are initially formed, but these appear to deteriorate over time and become more disorganised.

Centrioles in Cep135^c04199 mutant spermatocytes are shorter than in wild-type. These centrioles are also wider than found in wild-type. The diameter of the central hub of the cartwheel is unaffected.

Mutant Cep135^c04199 wing disc cells exhibit shorter and wider centrioles than found in wild-type. The majority of the mutant centrioles exhibit a perturbed cartwheel structure, displaying an abnormally undulating appearance, with the remaining centrioles displaying only remnants of a cartwheel structure. As in spermatocytes, the inner centriole diameter is enlarged.

There are no obvious defects in embryonic centrioles in Cep135^c04199 mutants compared to wild-type, although the diameter of the inner centriole is slightly enlarged in the mutant, indicating that the centrioles are not entirely normal even when only a few hours old.

Cep135^c04199/Df(3L)Brd15 males are sterile. Spermatocytes have shorter centrioles than normal and show premature centriole disengagement associated with defects in meiosis I of spermatogenesis. The number of axonemes in mutant 64 spermatid cysts is similar to that seen in wild type. However, the central microtubule pair is absent from the mutant axonemes.

Cep135^c04199/Df(3L)ED218 flies have shorter centrioles than controls and occasionally have mislocalized proximal centriole-like (PCL) structures. However, PCL formation is unaffected.

Homozygous adult males derived from homozygous females show normal centriole duplication in the spermatocytes.

Neuroblasts in homozygous larvae lacking either zygotic or zygotic plus maternal Cep135 function produce robust microtubule asters and assemble apparently normal mitotic spindles.

Mutant adults have no obvious uncoordinated movement.

Homozygous, hemizygous and Cep135^c04199/Cep135^f01951 males develop mature spermatozoa, but the sperm have no motile flagella.

Homozygous dividing spermatocytes have significantly shorter centrioles than normal. These centrioles appear to assemble astral microtubules normally, and onion stage spermatids have a single round nucleus associated with a nebenkern of similar shape and size as in wild type. The basal bodies of elongating homozygous spermatids are shorter than normal.

Sperm tails do assemble in the homozygous males. Approximately 48% of axonemes in homozygous elongating spermatids lack the two central tubules. At later stages of spermatid elongation, the mutant axonemes do develop the nine supplementary accessory tubules and nine radial spokes with secondary fibrils that are seen in wild type, but 56% of the axonemes have no central tubules, 27% have only one central tubule, 15% have a central amorphous electron-dense mass, and only 2% have the normal complement of two central tubules. The association of the axoneme with the nebenkern is maintained in mutants, but the orientations of spermatid tails within the cyst are irregular. The nuclei are still connected to the axoneme by the basal bodies in mutant males. The nuclei in the spermatid bundles are moderately dispersed along the bundle in the mutant males (in wild type they are packed at one end of the bundle).