FlyBase Allele Report: Dmel\boi[e01708]

General Information

Symbol

Dmel\boi^e01708

Species

D. melanogaster

Name

FlyBase ID

FBal0185919

Feature type

allele

Associated gene

Dmel\boi

Associated Insertion(s)

PBac{RB}boi^e01708

Carried in Construct

Also Known As

boi^e

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - molecular evidence

Mutagen

piggyBac activity

Nature of the Allele

Allele class

hypomorphic allele - molecular evidence

(Hartman et al., 2010)

Mutagen

piggyBac activity

(Gene Disruption Project members and Exelixis, 2005)

Progenitor genotype

Associated Insertion(s)

PBac{RB}boi^e01708

Cytology

Description

Allele components

Component

Use(s)

Inserted element

PBac{RB}

gene trap

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

X:2,465,487..2,465,487 [+]

(Hartman et al., 2010, Gene Disruption Project members and Exelixis, 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

hyperplasia

(Hartman et al., 2010)

hyperplasia, with Scer\GAL4^bab1-Pgal4-2, boi^ΔFN1.UAS

(Hartman et al., 2010)

hyperplasia (with boi^d04197)

(Hartman et al., 2010)

increased occurrence of cell division | adult stage | female

(Hartman et al., 2010)

increased occurrence of cell division | adult stage | female (with boi^d04197)

(Hartman et al., 2010)

Phenotype Manifest In

follicle cell

(Hartman et al., 2010)

follicle cell (with boi^d04197)

(Hartman et al., 2010)

follicle stem cell

(Hartman et al., 2010)

follicle stem cell, with Scer\GAL4^bab1-Pgal4-2, boi^ΔFN1.UAS

(Hartman et al., 2010)

follicle stem cell (with boi^d04197)

(Hartman et al., 2010)

stalk follicle cell

(Hartman et al., 2010)

stalk follicle cell (with boi^d04197)

(Hartman et al., 2010)

Detailed Description

Statement

Reference

Flies bearing homozygous or trans-heterozygous mutations in boi^d04197 and boi^e01708 exhibit excess follicle cells that accumulate between developing egg chambers. Although stalks of follicle cells between egg chambers in wild-type flies contain nine follicle cells on average, the mutant stalks have twice as many cells. Additional defects associated with excessive follicle cell production, including improper egg chamber packaging, delayed differentiation, and follicle cell polarity defects are also observed. Follicle stem cells (FSCs) in the mutants proliferate more frequently than in wild-type. The number of dividing pre-follicle cells derived from mutant FSCs is also higher than wild-type.

Expression of boi^{ΔFN1.Scer\UAS} in apical follicle cells under the control of Scer\GAL4^bab1-Pgal4-2 fails to rescue the boi^e01708 mutant follicle stem cell hyper-proliferation phenotype.

(Hartman et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

boi^e01708 has hyperplasia phenotype, non-enhanceable by ihog[+]/ihog^e02142

(Hartman et al., 2010)

Suppressed by

Statement

Reference

boi^e01708 has hyperplasia phenotype, suppressible by hh[+]/hh^AC

(Hartman et al., 2010)

boi^e01708 has hyperplasia phenotype, suppressible by hh^JF01804/Scer\GAL4^bab1-Pgal4-2

(Hartman et al., 2010)

boi^e01708 has hyperplasia phenotype, suppressible by smo[+]/smo³

(Hartman et al., 2010)

boi^e01708 has hyperplasia phenotype, suppressible by smo^RNAi.UAS.WIZ/Scer\GAL4^109-30

(Hartman et al., 2010)

boi^e01708 has hyperplasia phenotype, suppressible by ci^JF01715/Scer\GAL4^109-30

(Hartman et al., 2010)

boi^e01708 has hyperplasia phenotype, suppressible by ihog^G13202/Scer\GAL4^bab1-Pgal4-2

(Hartman et al., 2010)

NOT suppressed by

Statement

Reference

boi^e01708 has hyperplasia phenotype, non-suppressible by ihog[+]/ihog^e02142

(Hartman et al., 2010)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

boi^e01708 has stalk follicle cell phenotype, non-enhanceable by ihog[+]/ihog^e02142

(Hartman et al., 2010)

boi^e01708 has follicle cell phenotype, non-enhanceable by ihog[+]/ihog^e02142

(Hartman et al., 2010)

boi^e01708 has follicle stem cell phenotype, non-enhanceable by ihog[+]/ihog^e02142

(Hartman et al., 2010)

Suppressed by

Statement

Reference

boi^e01708 has follicle stem cell phenotype, suppressible by hh[+]/hh^AC

(Hartman et al., 2010)

boi^e01708 has follicle stem cell phenotype, suppressible by hh^JF01804/Scer\GAL4^bab1-Pgal4-2

(Hartman et al., 2010)

boi^e01708 has follicle stem cell phenotype, suppressible by smo[+]/smo³

(Hartman et al., 2010)

boi^e01708 has follicle stem cell phenotype, suppressible by smo^RNAi.UAS.WIZ/Scer\GAL4^109-30

(Hartman et al., 2010)

boi^e01708 has follicle stem cell phenotype, suppressible by ci^JF01715/Scer\GAL4^109-30

(Hartman et al., 2010)

NOT suppressed by

Statement

Reference

boi^e01708 has stalk follicle cell phenotype, non-suppressible by ihog[+]/ihog^e02142

(Hartman et al., 2010)

boi^e01708 has follicle cell phenotype, non-suppressible by ihog[+]/ihog^e02142

(Hartman et al., 2010)

boi^e01708 has follicle stem cell phenotype, non-suppressible by ihog[+]/ihog^e02142

(Hartman et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

The penetrance and severity of the follicle cell phenotypes of homozygous boi^e01708 flies are not affected by ihog^e02142/+.

Expression of ihog^G13202 in apical follicle cells under the control of Scer\GAL4^bab1-Pgal4-2 suppresses the boi^e01708 mutant follicle stem cell phenotype.

The follicle stem cell hyper-proliferation and excess follicle cell accumulation phenotypes are suppressed in boi^e01708 mutants by hh^AC/+.

Expression of hh^JF01804 in apical follicle cells under the control of Scer\GAL4^bab1-Pgal4-2 suppresses the follicle stem cell hyper-proliferation and excess follicle cell accumulation phenotypes in boi^e01708 mutants.

The follicle stem cell hyper-proliferation and excess follicle cell accumulation phenotypes are suppressed in boi^e01708 mutants by smo³/+.

Expression of smo^{dsRNA.Scer\UAS.WIZ} under the control of Scer\GAL4^109-30 suppresses the boi^e01708 mutant follicle stem cell phenotype.

Expression of ci^JF01715 under the control of Scer\GAL4^109-30 suppresses the boi^e01708 mutant follicle stem cell phenotype.

(Hartman et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

boi^e01708 is rescued by boi^UAS.cHa

(Hartman et al., 2010)

boi^e01708 is rescued by boi^ΔFN2.UAS

(Hartman et al., 2010)

Not rescued by

boi^e01708 is not rescued by boi^ΔFN1.UAS

(Hartman et al., 2010)

Comments

Expression of boi^{ΔFN1.Scer\UAS} in apical follicle cells under the control of Scer\GAL4^bab1-Pgal4-2 fails to rescue the boi^e01708 mutant follicle stem cell hyper-proliferation phenotype.

Expression of boi^Scer\UAS.cHa in apical follicle cells under the control of Scer\GAL4^bab1-Pgal4-2 rescues the boi^e01708 mutant follicle stem cell hyper-proliferation phenotype.

Expression of boi^{ΔFN2.Scer\UAS} in apical follicle cells under the control of Scer\GAL4^bab1-Pgal4-2 rescues the boi^e01708 mutant follicle cell phenotypes.

(Hartman et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

86015

PBac{RB}boi^e01708 w¹¹¹⁸

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CG32796^e01708

boi^e01708

(Hartman et al., 2013, Hartman et al., 2010)

boi^e

(Hartman et al., 2013, Hartman et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

Open Close