Imprecise excision of P{SUPor-P}CG11739KG9756 has led to deletion of approximately 5kb from the P{SUPor-P}CG11739KG9756 insertion site into the Cont coding sequence.
axolemma & nerve
embryonic epidermis & pleated septate junction
scolopidium & abdominal lateral pentascolopidial chordotonal organ lch5
septate junction & peripheral glial cell
In Contex956 homozygotes lateral pentascolopidial chordotonal organs have a disorganised morphology and rounded, rather than fusiform scolopales. Unlike in wild-type embryos, lateral pentascolopidial chordotonal organs in these embryos fail to exclude a 10kDa dextran dye. Ultrastructural analyses of peripheral nerves in these mutants at late stages of embryogenesis show that, while glial membranes are present and normally spaced around these nerves, the associated septate junctions linking inner and outer glial sheath cells are missing and the boundary between the inner glial membrane and axolemma is abnormally fuzzy.
In the epidermis of late stage Contex956/Contex956 embryos, the strands of septa that form the septate junctions on the apical side of adherens junctions are usually present but disrupted. The strands present consist of small clusters rather than the long ladder-like structures seen in wild-type septate junctions, although the spacing of septa in these clusters is normal and the strands are narrower than wild-type. These mutants also lack electron-dense intramembranous particles seen in wild-type septate junctions. Unlike wild-type, the epithelia of the salivary glands fail to exclude rhodamine-dextran dye (approximately 10 kDa) injected into the body cavity of late embryos from the salivary gland lumen.