FB2024_03 , released June 25, 2024
Allele: Dmel\Contex956
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General Information
Symbol
Dmel\Contex956
Species
D. melanogaster
Name
FlyBase ID
FBal0184320
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Caused by aberration
Cytology
Description

Imprecise excision of P{SUPor-P}CG11739KG9756 has led to deletion of approximately 5kb from the P{SUPor-P}CG11739KG9756 insertion site into the Cont coding sequence.

Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DNA sequence
Protein sequence
 
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

axolemma & nerve

embryonic epidermis & pleated septate junction

scolopidium & abdominal lateral pentascolopidial chordotonal organ lch5

septate junction & peripheral glial cell

Detailed Description
Statement
Reference

In Contex956 homozygotes lateral pentascolopidial chordotonal organs have a disorganised morphology and rounded, rather than fusiform scolopales. Unlike in wild-type embryos, lateral pentascolopidial chordotonal organs in these embryos fail to exclude a 10kDa dextran dye. Ultrastructural analyses of peripheral nerves in these mutants at late stages of embryogenesis show that, while glial membranes are present and normally spaced around these nerves, the associated septate junctions linking inner and outer glial sheath cells are missing and the boundary between the inner glial membrane and axolemma is abnormally fuzzy.

In the epidermis of late stage Contex956/Contex956 embryos, the strands of septa that form the septate junctions on the apical side of adherens junctions are usually present but disrupted. The strands present consist of small clusters rather than the long ladder-like structures seen in wild-type septate junctions, although the spacing of septa in these clusters is normal and the strands are narrower than wild-type. These mutants also lack electron-dense intramembranous particles seen in wild-type septate junctions. Unlike wild-type, the epithelia of the salivary glands fail to exclude rhodamine-dextran dye (approximately 10 kDa) injected into the body cavity of late embryos from the salivary gland lumen.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Cont+t7 rescues survival of Contex956/Contex956 animals to adulthood.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (4)