Expression of DifScer\UAS.cIa, driven by Scer\GAL4hs.PB at 37oC (as a 1 hour pulse) is lethal.
Overexpression of DifScer\UAS.cIa under the control of Scer\GAL4Cg.PA causes a significant increase in the amount of hemocytes, including lamellocyte and plasmatocyte levels, in the hemolymph of third instar larvae compared to wild type.
Scer\GAL4hs.PB, DifUAS.cIa, Dif1 is a suppressor of lethal | larval stage phenotype of dl1
DifUAS.cIa, Scer\GAL4Cg.PA has lamellocyte | increased number phenotype, non-enhanceable by dlUAS.cHa, Scer\GAL4Cg.PA
DifUAS.cIa, Scer\GAL4Cg.PA has embryonic/larval hemocyte | increased number phenotype, non-enhanceable by dlUAS.cHa, Scer\GAL4Cg.PA
DifUAS.cIa, Scer\GAL4Cg.PA has embryonic/larval plasmatocyte | increased number phenotype, non-enhanceable by dlUAS.cHa, Scer\GAL4Cg.PA
DifUAS.cIa, Scer\GAL4Cg.PA has lamellocyte | increased number phenotype, non-suppressible by dlUAS.cHa, Scer\GAL4Cg.PA
DifUAS.cIa, Scer\GAL4Cg.PA has embryonic/larval plasmatocyte | increased number phenotype, non-suppressible by dlUAS.cHa, Scer\GAL4Cg.PA
DifUAS.cIa, Scer\GAL4Cg.PA has embryonic/larval hemocyte | increased number phenotype, non-suppressible by dlUAS.cHa, Scer\GAL4Cg.PA
Ubiquitous expression of DifScer\UAS.cIa, driven by Scer\GAL4hs.PB at 25oC (without heat shock) fully rescues the lethality of Dif1 dl1 double mutants.
Overexpression of DifScer\UAS.cIa and dlScer\UAS.cHa, both under the control of Scer\GAL4Cg.PA, causes the same hemocyte phenotype as when only DifScer\UAS.cIa is expressed.
Y.T. Ip