FB2024_03 , released June 25, 2024
Allele: Dmel\MbsN300.UAS
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General Information
Symbol
Dmel\MbsN300.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0182792
Feature type
allele
Associated gene
Dmel\Mbs
Associated Insertion(s)
Carried in Construct
P{UAS-Mbs.N300}
Also Known As
UAS-MbsN300, UAS-mbsN300
Key Links
Transgenic product class
inframe_deletion
(Lee and Treisman, 2004)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Lee and Treisman, 2004)
Mutagen
in vitro construct
(Lee and Treisman, 2004)
Progenitor genotype
Carried in construct
P{UAS-Mbs.N300}
Cytology
Description

Expression of a constitutively active form of Mbs, which consists of the N-terminal 300 amino acids, is governed by UASt regulatory sequences.

(Lee and Treisman, 2004)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Mbs
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Stage 16 MbsN300.Scer\UAS embryos under the control of the Scer\GAL4how-24B driver show laterality defects in the proventriculus and anterior midgut. The laterality of the other parts of the embryonic gut, including the hindgut and the posterior part of the midgut is normal in these mutants. The proventriculus and the anterior midgut do not rotate (as in wild-type) in these mutants at stages 15 to 17.

      Stage 16 MbsN300.Scer\UAS embryos under the control of either Scer\GAL4NP5021 or Scer\GAL4NP221 do not show any loss of rotation in the proventriculus and anterior midgut.

      (Okumura et al., 2010)

      Expression of MbsN300.Scer\UAS in larval epidermal cells, under the control of Scer\GAL4NP0056, results in a significant proportion of these cells failing to extrude and remaining within the abdominal epidermis at postmetamorphosis periods. This results in cuticular clefts in the adult abdomen.

      (Ninov et al., 2007)

      Expression of MbsN300.Scer\UAS, under the control of Scer\GAL4da.G32, causes lethality at or before the pupal stage. When its expression is driven in the eye disc by Scer\GAL4ey.PH or Scer\GAL4GMR.PF, there are no apparent defects in eye receptor differentiation.

      (Lee and Treisman, 2004)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhancer of
      Statement
      Reference

      MbsN300.UAS/Scer\GAL4GMR.PU is an enhancer of retina phenotype of eys1, prom[+]/prom1

      (Nie et al., 2014)
      Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of MbsN300.Scer\UAS under the control of Scer\GAL4GMR.PU enhances the rhabdomere fusion seen in eys1 prom1 double heterozygotes.

      (Nie et al., 2014)

      Overexpression of both rokCAT.Scer\UAS and MbsN300.Scer\UAS, under the control of Scer\GAL4da.G32, allow ~30% of flies to survive to adulthood, instead of dying as pupae.

      (Lee and Treisman, 2004)
      Xenogenetic Interactions
      Statement
      Reference

      Co-expression of MbsN300.Scer\UAS and BacA\p35Scer\UAS.cHa in the dorsal compartment of the wing disc (under the temperature-regulated control of Scer\GAL4ap-md544 by Scer\GAL80ts.αTub84B) does not alter apical-basal cell length in mutant cells compared with control cells.

      The shorter cell length observed in wing disc tissue expressing DadScer\UAS.cTa and BacA\p35Scer\UAS.cHa (under the temperature-regulated control of Scer\GAL4ap-md544 by Scer\GAL80ts.αTub84B), is rescued by co-expression of MbsN300.Scer\UAS.

      (Widmann and Dahmann, 2009)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Encodes a constitutively activated form of Mbs.

      (Okumura et al., 2010)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      MbsN300.Scer\UAS
      (Pulido Companys et al., 2020)
      MbsN300.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (13)