Insertion of the sequence SVRQ after residue 272. This insertion is in the splice site between exons 4 and 5.
NNNGTNNGNCAR
Reporte as the insertion of a four codons encoding SVRQ at the splice site after mys amino acid S272. Same mutation as in mys[b50]. Position of mutation on reference sequence inferred by FlyBase curator based on author statement.
20% of mutant males raised at 18-22[o]C through the first or second larval instar stages and then shifted to 28[o]C prior to pupal development have an under-rotated genitalia phenotype. The frequency of rotation defects is reduced if the flies are reared at 22[o]C.
Strong allele.
Selected as: a mutation that permits the survival of an F1 male.
