pyx³ mutant larvae have minimally reduced rolling (escape) behavior in response to noxious temperature increases, compared to controls.

pyx³ mutant adults show normal preference for less solid sucrose-agarose food than a harder one (higher agarose conc., same sucrose content) one in a two-way food choice assay.

pyx³ mutant adults have slightly, but significantly, reduced aversive shock, but not temperature, olfactory memory, compared to controls.

The gustatory aversion of mutant flies to 6mM camphor in a two way-choice test is not significantly different from that seen in wild-type flies.

pyx³ mutants do not exhibit a noxious heat avoidance phenotype.

In contrast to adults, pyx³ mutant larvae show delays and defects in responding to thermal pain, indicating defects in thermal nociception.

Mutant third instar larvae show the same preference for 17.5[o]C over 14[o]C in a two-way choice test as do wild-type controls.

pyx³ mutants display impaired negative geotaxis behaviour.

The Johnston's organ of pyx³ mutant flies show impaired neural activity in response to body rotation.

pyx³ mutants show auditory responses that are similar to that of wild-type.

pyx³ mutants retain a preference for 18[o]C (i.e. are thermotactic).

pyx³ mutants have similar temperature preferences to wild-type flies, although their preferred mean temperature (24.5⁰C) is lower than that of wild-type flies (25.3⁰C). More than 60% of pyx³ mutant flies are paralyzed within 3 minutes of being placed on an aluminum block of 40⁰C, whereas only 9% of wild-type flies are paralyzed under these conditions.