Under starvation conditions, Arl8^e00336 homozygous third instar larvae exhibit fewer and smaller autolysosomes, and more autophagosomes in fat cells compared to controls.

Arl8^e00336/Df(3R)BSC222 larval Garland cells show enlarged vacuoles and show postendocytic trafficking defects (e.g. endocytozed proteins fail to be efficiently incorporated into Lysotracker-positive autolysosomes), as compared to controls.

Arl8^e00336 homozygous third instar larvae exhibit posterior paralysis resulting in a characteristic tail flipping phenotype when compared to controls.

Arl8^e00336 homozygous larvae exhibit reduced number of synaptic boutons per synapse at the neuromuscular junction of the muscle 6/7, abdominal segment A2, and smaller synapses in axons compared to controls.