[84E8-84E8];[84F6-84F6];
A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
84E8;84F6
Breakpoint from FlyBase's release 5 sequence location of progenitor insertion.
The presence of P+PBac{XP5.WH5}BSC222 was verified using the PCR methods and primers described in FBrf0175003.
Exelixis, Inc. determined the insertion site of P{XP}d08394 to be Release 3 genomic coordinate 4076070 on chromosome arm 3R. The Gene Disruption project determined the insertion site of P{XP}d08394 to be Release 3 genomic coordinate 4076158 on arm 3R. This corresponds to 84F6 on both the Release 3 and Release 4 genome maps. The predicted position of PBac{WH}CG10435f03495 on the Release 4 map is 84E8.
Consequently, the cytological breakpoints of Df(3R)BSC222 are predicted to be 84E8;84F6.