FlyBase Allele Report: Dmel\Ogg1[f08013]

Insertion integrated 277 bp upstream of the Ogg1 ATG codon. Scer\UAS within the transposon are in the same orientation as the Ogg1 transcription unit, thus allowing Scer\UAS-regulated over-expression.

(Yasukawa et al., 2015)

Allele components

Component

Use(s)

Inserted element

PBac{WH}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

X:8,690,409..8,690,409 [-]

(Gene Disruption Project members and Exelixis, 2005, Bloomington Drosophila Stock Center, 2004.11.6)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal DNA repair | adult stage

(Okumura et al., 2019)

abnormal oxidative stress response

(Yasukawa et al., 2015)

abnormal oxidative stress response, with Scer\GAL4^Act5C.PI

(Yasukawa et al., 2015)

fertile

(Bloomington Drosophila Stock Center, 2004.11.6)

long lived, with Scer\GAL4^Act5C.PI

(Yasukawa et al., 2015)

viable

(Bloomington Drosophila Stock Center, 2004.11.6)

Phenotype Manifest In

adult midgut

(Okumura et al., 2019, Yasukawa et al., 2015)

adult midgut, with Scer\GAL4^Act5C.PI

(Yasukawa et al., 2015)

Detailed Description

Statement

Reference

The midgut of Ogg1^f08013 hemizygous male adults exhibit increased DNA damage (accumulation of 8-oxoG and γH2AvD).

(Okumura et al., 2019)

Mutant (Ogg1^f08013) adult flies exhibit hyper-accumulation of 8-oxo-guanines in midgut cells after paraquat feeding. However, the lifespan of these mutant flies in this oxidative stress assay is not decreased and is actually longer than controls.

Adult flies over-expressing Ogg1 (Scer\GAL4^Act5C.PI, Ogg1^f08013) exhibit a significant decrease in 8-oxo-guanines in midgut cells after paraquat feeding.

Scer\GAL4^Act5C.PI-mediated expression of Ogg1^f08013 results in a significantly longer lifespan than controls, either under normal feeding conditions or when adults are fed paraquat.

(Yasukawa et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Ogg1^f08013 has abnormal DNA repair phenotype, enhanceable by RpS3[+]/RpS3¹

(Okumura et al., 2019)

Ogg1^f08013 has abnormal DNA repair phenotype, enhanceable by RpS3[+]/RpS3²

(Okumura et al., 2019)

Enhancer of

Statement

Reference

Ogg1^f08013 is an enhancer of abnormal DNA repair | adult stage | dominant phenotype of RpS3¹

(Okumura et al., 2019)

Ogg1^f08013 is an enhancer of abnormal DNA repair | adult stage | dominant phenotype of RpS3²

(Okumura et al., 2019)

Ogg1^f08013/Ogg1^f08013 is an enhancer of bang sensitive phenotype of Sod2ⁿ²⁸³/Sod2^KG06854

(Itsara et al., 2014)

Ogg1^f08013/Ogg1^f08013 is an enhancer of short lived phenotype of Sod2ⁿ²⁸³/Sod2^KG06854

(Itsara et al., 2014)

Phenotype Manifest In

Enhanced by

Statement

Reference

Ogg1^f08013 has adult midgut phenotype, enhanceable by RpS3[+]/RpS3¹

(Okumura et al., 2019)

Ogg1^f08013 has adult midgut phenotype, enhanceable by RpS3[+]/RpS3²

(Okumura et al., 2019)

Enhancer of

Statement

Reference

Ogg1^f08013 is an enhancer of adult midgut phenotype of RpS3¹

(Okumura et al., 2019)

Ogg1^f08013 is an enhancer of adult midgut phenotype of RpS3²

(Okumura et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

19122

w¹¹¹⁸ PBac{WH}Ogg1^f08013

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Ogg1^f08013

(Okumura et al., 2019, Yasukawa et al., 2015, Itsara et al., 2014)

Ogg1^ins

(Itsara et al., 2014)

Ppt1^f08013

(Bloomington Drosophila Stock Center, 2004.11.6)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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