FB2024_03 , released June 25, 2024
Allele: Dmel\Ptp69D10
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General Information
Symbol
Dmel\Ptp69D10
Species
D. melanogaster
Name
FlyBase ID
FBal0152288
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Nature of the Allele
Allele class
Progenitor genotype
Cytology
Description

Nucleotide substitution: T?A. Amino acid replacement: V134D.

Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Nucleotide change:

T12736453A

Reported nucleotide change:

T?A

Amino acid change:

V134D | Ptp69D-PA; V134D | Ptp69D-PB

Reported amino acid change:

V134D

Variant Molecular Consequences
Associated Sequence Data
DNA sequence
Protein sequence
 
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

In Ptp69D1/Ptp69D10 adults, thoracic mechanosensory neurons exhibit decreased connectivity: branch loss; full extension of the contralateral branch, but complete loss of synaptic arborizations; decreased presynapse number; and contralateral branch arrest at the midline/on the ipsilateral side.

At 25oC, the survival rates of homozygotes are: adult survival - 77%, eclosion - 80%, pupation 03%. At 18oC, the survival rates of homozygotes are: adult survival - 85%, eclosion - 93%, pupation 88%. Temperature shift experiments indicate that to survive after eclosion, Ptp69D1/Ptp69D10 animals must be shifted to the permissive temperature (18oC) from the restrictive temperature (25oC) as early pupae. The rate of eclosion drops from 100% at 18oC to 75% when Ptp69D1/Ptp69D10 animals are reared at 25oC for their first 24 hours and drops further to 40% if the animals are raised at 25oC until the early pupal stage. Ptp69D10 embryos show motor axon defects; 33% of ISNb axons, 14% of ISN axons, 22% of SNa axons and 4% of SNc axons show defects. 9% of ISNb axons show a complete bypass phenotype and 15% show a partial bypass phenotype.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference

Ptp69D10 has abnormal neuroanatomy phenotype, suppressible by Ptp99A[+]/Ptp99A1

Phenotype Manifest In
Suppressed by
Statement
Reference

Ptp69D10 has larval intersegmental nerve phenotype, suppressible by Ptp99A[+]/Ptp99A1

Additional Comments
Genetic Interactions
Statement
Reference

The ISNb bypass phenotype seen in Ptp69D10 homozygous embryos is largely suppressed by one copy of Ptp99A1.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (5)