Nucleotide substitution: T?A. Nucleotide substitution: G?A. Nucleotide substitution: G?T. Amino acid replacement: W171R. Amino acid replacement: G757E. Amino acid replacement: R903L.
T12736563A
T?A
W171R | Ptp69D-PA; W171R | Ptp69D-PB
W171R
One of three nucleotide substitutions in Ptp69D coding region in mutant.
G12738384A
G?A
G758E | Ptp69D-PA; G758E | Ptp69D-PB
G757E
One of three nucleotide substitutions in Ptp69D coding region in mutant.
G12738945T
G?T
R903L | Ptp69D-PA; R902L | Ptp69D-PB
R903L
One of three nucleotide substitutions in Ptp69D coding region in mutant.
lethal | heat sensitive (with Df(3L)8ex34)
lethal | heat sensitive (with Ptp69D1)
lethal | heat sensitive (with Ptp69D4)
lethal | heat sensitive (with Ptp69D8ex25)
At 25oC, the survival rates of homozygotes are: adult survival - 0%, eclosion - 1%, pupation 34%. At 18oC, the survival rates of homozygotes are: adult survival - 59%, eclosion - 87%, pupation 90%. Temperature shift experiments indicate that to survive after eclosion, Ptp69D12/Df(3L)8ex34 animals must be at the mid pupal stage prior to the shift to the restrictive temperature (25oC). The rate of eclosion drops from more than 80% at 18oC to less than 20% when Ptp69D12/Df(3L)8ex34 animals are shifted to the nonpermissive temperature prior to the onset of pupation. Temperature shift experiments also reveal a requirement for Ptp69D function during late embryonic development. Ptp69D12 embryos show motor axon defects; 18% of ISNb axons, 9% of ISN axons, 7% of SNa axons and 6% of SNc axons show defects.