Construct: Amino acid replacement: S?A.
eye | ectopic, with Scer\GAL4dpp.blk1
The average penetrance of ectopic eye induction in eyaS-A.Scer\UAS; Scer\GAL4dpp.blk1 flies is 19% (over 8 independent P{UAS-eyaS-A} insertions). Ectopic eye patches seen in these flies are smaller than those seen in eyaScer\UAS.cHa; Scer\GAL4dpp.blk1 flies. i.e. - the penetrance of ectopic eye phenotypes is lower than that seen in eyaScer\UAS.cHa; Scer\GAL4dpp.PH flies. The penetrance of ectopic eye induction in eyaS-A.Scer\UAS; Scer\GAL4dpp.blk1 flies is reduced from 42% (n=306) to 20% (n=304) by heterozygosity for eyaG130 (data from a single, 'strong' P{UAS-eyaS-A} insertion). eyaS-A.Scer\UAS; Scer\GAL4dpp.PH flies have highly variable wing defects, including ectopic bristles on L3 and in more extreme cases, blistering, and defects in overall shape: Proportion with blistered wings = 14%; proportion with only mild wing defects = 71%, while 15% have wild-type wings. These flies also have increased numbers of scutellar machrochaete (average= 5.6, compared to 4 in wild-type). Expressivity of all of these phenotypes is lower than for eyaScer\UAS.cHa; Scer\GAL4dpp.PH flies. Note: all of the above data are averages from 8 independent P{UAS-eyaS-A} insertions.
Scer\GAL4dpp.blk1, eyaS-A.UAS has eye | ectopic phenotype, suppressible | partially by rl[+]/rlS-135
The penetrance of ectopic eye induction in eyaS-A.Scer\UAS; Scer\GAL4dpp.blk1 flies is reduced from 42% (n=306) to 13% (n=310) by heterozygosity for rlS-135 (data from a single, 'strong' P{UAS-eyaS-A} insertion).
These mutations of putative MAPK phosphorylation sites eliminate in vitro phosphorylation by rl, Mpk2 and p38b.