FlyBase Allele Report: Dmel\verm[KG07819]

General Information

Symbol

Dmel\verm^KG07819

Species

D. melanogaster

Name

FlyBase ID

FBal0148139

Feature type

allele

Associated gene

Dmel\verm

Associated Insertion(s)

P{SUPor-P}verm^KG07819

Carried in Construct

Also Known As

verm^KG

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Luschnig et al., 2006)

Mutagen

P-element activity

(Gene Disruption Project members, 2001-)

Progenitor genotype

Associated Insertion(s)

P{SUPor-P}verm^KG07819

Cytology

Description

P{SUPor-P}LCBP1^KG07819 is inserted 100bp upstream of the LCBP1 translation start site.

(Wang et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:19,695,124..19,695,124 [+]

(Förster and Luschnig, 2012, Cooper et al., 2010, Luschnig et al., 2006, Wang et al., 2006, Gene Disruption Project members, 2001-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during embryonic stage

(Luschnig et al., 2006)

some die during embryonic stage

(Luschnig et al., 2006)

Phenotype Manifest In

cuticle

(Luschnig et al., 2006)

cuticle | embryonic stage 16

(Wang et al., 2006)

embryonic/first instar larval cuticle

(Luschnig et al., 2006)

embryonic/larval tracheal dorsal trunk

(Luschnig et al., 2006)

embryonic/larval tracheal dorsal trunk | embryonic stage 16

(Wang et al., 2006)

embryonic/larval tracheal section

(Luschnig et al., 2006)

embryonic/larval tracheal system

(Wang et al., 2006)

Detailed Description

Statement

Reference

LCBP1^KG07819 mutants show expanded and deformed cuticles, associated with a lack of cuticle rigidity.

(Luschnig et al., 2006)

Stage 16 LCBP1^KG07819 mutant embryos display excessive elongation and convolutions of the tracheal dorsal trunks, which become gradually more pronounced in later stages. This defect becomes larger ameliorated in mutants expressing LCBP1^Scer\UAS.D in the trachea (under the control of Scer\GAL4^btl.PS), suggesting that LCBP1 restricts tube elongation.

Stage 16 LCBP1^KG07819 mutant embryos display irregular and elongated tracheal cell shapes compared to wild-type. Wild-type dorsal trunks at stage 16 consist of a homogeneously packed luminal matrix of parallel fibrils. The orientation and assembly of the fibrils is distorted in LCBP1^KG07819.

In wild-type, tracheal tubes consist of tightly packed luminal chitin polymers. However, in homozygous LCBP1^KG07819 mutants these structures appear diffuse and radially expanded.

Early events of tracheal cuticle assembly, like the apical deposition of the epicuticle layers at early stage 16 are unaffected in LCBP1^KG07819 mutants, as is the `ladder' of sepate junctions. However, cuticle abnormalities, such as irregular taenidia, reduced procuticle deposition, and aberrant apical membranes are present at late stage 16.

(Wang et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

verm^KG07819/Df(3L)Exel6135 has embryonic/larval tracheal dorsal trunk phenotype, enhanceable by serp^e02821

(Luschnig et al., 2006)

verm^KG07819/Df(3L)Exel6135 has embryonic/larval tracheal section phenotype, enhanceable by serp^e02821

(Luschnig et al., 2006)

verm^KG07819/Df(3L)Exel6135 has embryonic/first instar larval cuticle phenotype, enhanceable by serp^e02821

(Luschnig et al., 2006)

verm^KG07819/Df(3L)Exel6135 has cuticle phenotype, enhanceable by serp^e02821

(Luschnig et al., 2006)

Enhancer of

Statement

Reference

verm^KG07819 is an enhancer of embryonic/first instar larval cuticle phenotype of serp^e02821

(Luschnig et al., 2006)

verm^KG07819 is an enhancer of cuticle phenotype of serp^e02821

(Luschnig et al., 2006)

verm^KG07819 is an enhancer of embryonic/larval tracheal dorsal trunk phenotype of serp^e02821/Df(3L)Exel6135

(Luschnig et al., 2006)

verm^KG07819 is an enhancer of embryonic/larval tracheal section phenotype of serp^e02821/Df(3L)Exel6135

(Luschnig et al., 2006)

verm^KG07819 is an enhancer of embryonic/first instar larval cuticle phenotype of serp^e02821/Df(3L)Exel6135

(Luschnig et al., 2006)

verm^KG07819 is an enhancer of cuticle phenotype of serp^e02821/Df(3L)Exel6135

(Luschnig et al., 2006)

verm^KG07819 is an enhancer of embryonic/larval tracheal dorsal trunk phenotype of serp^e02821

(Luschnig et al., 2006)

verm^KG07819 is an enhancer of embryonic/larval tracheal section phenotype of serp^e02821

(Luschnig et al., 2006)

Other

Statement

Reference

serp^e02821, verm^KG07819 has embryonic/larval tracheal section phenotype

(Förster and Luschnig, 2012)

serp^e02821, verm^KG07819 has embryonic/larval cuticle phenotype

(Gangishetti et al., 2012)

serp^e02821, verm^KG07819 has dorsal tracheal branch primordium phenotype

(Luschnig et al., 2006)

serp^e02821, verm^KG07819 has tracheal transverse connective primordium phenotype

(Luschnig et al., 2006)

verm^KG07819/Df(3L)Exel6135, serp^e02821 has dorsal tracheal branch primordium phenotype

(Luschnig et al., 2006)

verm^KG07819/Df(3L)Exel6135, serp^e02821 has tracheal transverse connective primordium phenotype

(Luschnig et al., 2006)

kkv^S017909, verm^KG07819 has embryonic/larval tracheal system phenotype

(Wang et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Stage 16 serp^e02821 verm^KG07819 double mutant embryos show excessively elongated tracheae with long apical cell profiles.

(Förster and Luschnig, 2012)

Loss of chitin orientation is seen in the cuticle of serp^e02821 verm^KG07819 larvae, although layering of the cuticle is not affected.

(Gangishetti et al., 2012)

serp^e02821 LCBP1^KG07819 double mutants display an enhanced tracheal phenotype. The dorsal trunk begins to elongate excessively at stage 15 and by stage 16 is approximately 40% longer than normal and highly convoluted. Similar effects are observed in other branches including the transverse connective, although the effects are not as pronounced in smaller-caliber branches, such as the dorsal branch. While the apical (inner) cell surfaces are elongated in serp^e02821 LCBP1^KG07819 mutants the basal (outer) side of tracheal cells does not appear to be similarly elongated and does not follow the tortuous path of the lumen. The tracheal pericellular-diffusion barrier is not compromised in serp^e02821 LCBP1^KG07819 mutants.

serp^e02821 LCBP1^KG07819 double mutants exhibit morphological defects in chitin structure, apparent by stage 14, several hours before the elongated-tube phenotype begins to manifest. In contrast to wild-type, in serp^e02821 LCBP1^KG07819 trachea the fibrous structure of the chitin cylinder is abolished and the surface of the cylinder is irregular. Also, the small gap between the chitin cylinder and the apical surface is absent in serp^e02821 LCBP1^KG07819 mutants. This double mutant also affects embryo body shape, presumably through altering the structure and rigidity of epidermal cuticle.

The phenotype of hemizygous serp^e02821 LCBP1^KG07819 embryos (in trans to Df(3L)Exel6135) is indistinguishable from homozygous serp^e02821 LCBP1^KG07819 embryos. In contrast to the dramatic effects of the mutations on tracheal length and shape, there is little or no effect of the mutations on the diameter of the tubes. The dorsal trunk exhibits its characteristic posterior to anterior taper and is of normal caliber except for slight constrictions that are occasionally observed near dorsal trunk fusion joints.

(Luschnig et al., 2006)

The tracheal tube phenotypes of kkv^s017909 LCBP1^KG07819 double mutants are identical to the ones of kkv^s017909.

(Wang et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

verm^76BDx-6

(Cook, 2008.12.1)

Rescued by

verm^KG07819 is rescued by Scer\GAL4^btl.PS/verm^UAS.D

(Wang et al., 2006)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

14660

y¹; P{SUPor-P}verm^KG07819 ry⁵⁰⁶/TM3, Sb¹ Ser¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CG8756^KG07819

LCBP1^KG07819

(Cook, 2008.12.1)

verm^KG07819

verm^KG

(Förster and Luschnig, 2012, Gangishetti et al., 2012, Luschnig et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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