FlyBase Allele Report: Dmel\E(bx)[Nurf301-2]

General Information

Symbol

Dmel\E(bx)^Nurf301-2

Species

D. melanogaster

Name

FlyBase ID

FBal0145005

Feature type

allele

Associated gene

Dmel\E(bx)

Associated Insertion(s)

Carried in Construct

Also Known As

Nurf301²

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Badenhorst et al., 2005)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q545term.

(Badenhorst et al., 2005)

Amino acid replacement: Q546term.

(Badenhorst et al., 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:244,132..244,132 [-]

Nucleotide change:

C244132T

Amino acid change:

Reported amino acid change:

Q545term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Badenhorst et al., 2005, Badenhorst et al., 2002)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of larval stage | recessive

(Badenhorst et al., 2005)

some die during larval stage | recessive

(Badenhorst et al., 2005)

Phenotype Manifest In

hemocyte | increased number

(Badenhorst et al., 2002)

melanotic mass

(Badenhorst et al., 2002)

melanotic mass (with E(bx)^Nurf301-3)

(Badenhorst et al., 2002)

melanotic mass (with E(bx)^Nurf301-4)

(Badenhorst et al., 2002)

melanotic mass (with E(bx)^ry122)

(Badenhorst et al., 2002)

polytene chromosome

(Badenhorst et al., 2002)

polytene chromosome (with Df(3L)3643)

(Bai et al., 2007)

polytene chromosome (with E(bx)^Nurf301-3)

(Badenhorst et al., 2002)

Detailed Description

Statement

Reference

The morphology of the male X polytene chromosome is severely disrupted in E(bx)^Nurf301-2/Df(3L)3643 mutants; the chromosome appears very wide at the expense of length compared to wild type.

(Bai et al., 2007)

Mutants fail to pupariate.

(Badenhorst et al., 2005)

The male X chromosome in homozygous mutants is highly aberrant. This is also seen in E(bx)^Nurf301-2/E(bx)^Nurf301-3 mutants. E(bx)^Nurf301-2/E(bx)^Nurf301-2, E(bx)^Nurf301-2/E(bx)^Nurf301-3, E(bx)^Nurf301-2/E(bx)^Nurf301-4 and E(bx)^Nurf301-2/E(bx)^ry122 mutant larvae exhibit melanotic tumours. The circulating hemocyte cell number in hemolymph isolated from homozygous mutants is increased considerably with respect to wild-type animals, having about 16000 per ul^-1, compared to about 4500 in wild-type.

(Badenhorst et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

E(bx)^Nurf301-2/E(bx)[+] is an enhancer of melanotic mass phenotype | adult stage | dominant phenotype of hop^Tum

(Kugler et al., 2011)

E(bx)^Nurf301-2 is an enhancer of visible | somatic clone phenotype of Iswi²

(Arancio et al., 2010)

E(bx)^Nurf301-2 is an enhancer of visible phenotype of Iswi^{K159R.UAS.Tag:HA}, Scer\GAL4^ey.PH

(Burgio et al., 2008)

Phenotype Manifest In

Enhancer of

Statement

Reference

E(bx)^Nurf301-2/E(bx)[+] is an enhancer of melanotic mass | adult stage phenotype of hop^Tum

(Kugler et al., 2011)

E(bx)^Nurf301-2 is an enhancer of eye | somatic clone phenotype of Iswi²

(Arancio et al., 2010)

E(bx)^Nurf301-2 is an enhancer of eye phenotype of Iswi^{K159R.UAS.Tag:HA}, Scer\GAL4^ey.PH

(Burgio et al., 2008)

E(bx)^Nurf301-2/E(bx)[+] is an enhancer of dorsal appendage | maternal effect phenotype of EcR^{B1-ΔC655.F645A.UAS}, Scer\GAL4^slbo.2.6

(Badenhorst et al., 2005)

E(bx)^Nurf301-2 is an enhancer of adult mesothoracic segment | anterior | ectopic phenotype of Ubx^6.28/Ubx^bx-8

(Badenhorst et al., 2002)

E(bx)^Nurf301-2 is an enhancer of adult metathoracic segment phenotype of Ubx^6.28/Ubx^bx-8

(Badenhorst et al., 2002)

E(bx)^Nurf301-2 is an enhancer of adult mesothoracic segment | anterior | ectopic phenotype of Ubx^6.28/Ubx^bx-34e

(Badenhorst et al., 2002)

E(bx)^Nurf301-2 is an enhancer of adult metathoracic segment phenotype of Ubx^6.28/Ubx^bx-34e

(Badenhorst et al., 2002)

E(bx)^Nurf301-2 is an enhancer of haltere phenotype of Ubx^6.28/Ubx^bx-34e

(Badenhorst et al., 2002)

E(bx)^Nurf301-2 is an enhancer of wing | ectopic phenotype of Ubx^6.28/Ubx^bx-34e

(Badenhorst et al., 2002)

E(bx)^Nurf301-2 is an enhancer of melanotic mass phenotype of hop^Tum

(Badenhorst et al., 2002)

Suppressor of

Statement

Reference

E(bx)^Nurf301-2 is a suppressor of wing phenotype of upd1^unspecified

(Badenhorst et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

The penetrance of the melanotic mass phenotype seen in hop^Tum/+ females is enhanced if they also carry one copy of E(bx)^Nurf301-2.

(Kugler et al., 2011)

E(bx)^Nurf301-2 enhances the eye defects seen in flies in which the eyes are homozygous for Iswi² in an otherwise heterozygous background (generated using the EGUF technique).

(Arancio et al., 2010)

Heterozygosity for E(bx)^Nurf301-2 strongly enhances the penetrance and severity of the wing margin defects seen in Df(1)N-54l9 heterozygotes.

(Kugler and Nagel, 2010)

The severity of the mutant eye phenotype caused by expression of Iswi^{K159R.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^ey.PH is enhanced by E(bx)^Nurf301-2.

(Burgio et al., 2008)

The frequency of abnormal dorsal appendages in embryos derived from females carrying EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^slbo.2.6 (23%) is increased if the females are also heterozygous for E(bx)^Nurf301-2 (70%).

(Badenhorst et al., 2005)

The addition of E(bx)^Nurf301-2 enhances the melanotic tumour phenotype seen in hop^Tum/+ larvae, so that over 60% exhibit tumours at 25^oC and 80% at 28^oC, in the case of E(bx)^Nurf301-2.

(Badenhorst et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

E(bx)^Nurf301-3

(Badenhorst et al., 2002)

E(bx)^Nurf301-4

(Badenhorst et al., 2002)

E(bx)^ry122

(Badenhorst et al., 2002)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

E(bx)^Nurf301-2

(Arancio et al., 2010, Burgio et al., 2008)

E(bx)^nurf301-2

Nurf301²

(Kugler et al., 2011, Kugler and Nagel, 2010, Bai et al., 2007, Badenhorst et al., 2005)

nurf301²

(Badenhorst et al., 2002)

Name Synonyms

Secondary FlyBase IDs

References (7)

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