FlyBase Allele Report: Dmel\Ubx[6.28]

General Information

Symbol

Dmel\Ubx^6.28

Species

D. melanogaster

Name

FlyBase ID

FBal0017486

Feature type

allele

Associated gene

Dmel\Ubx

Associated Insertion(s)

Carried in Construct

Also Known As

Ubx^6-28

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

X ray

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(McCormick et al., 1995, Andrew et al., 1994, Reuter and Scott, 1990, Reuter et al., 1990)

Mutagen

X ray

(Weinzierl et al., 1987, Bender et al., 1983, Capdevila and Garcia-Bellido, 1981)

Progenitor genotype

Cytology

Polytene chromosomes normal.

(Bender et al., 1983, Kerridge and Morata, 1982)

Description

Deletion of 32bp at the 5' end of exon 1 that causes a frame shift at codon 27 and a premature stop to translation.

(Reuter et al., 1990)

32bp deletion in the major 5' exon, introducing a frame shift into the open reading frame (ORF) after 27 codons, and resulting in the premature termination of the ORF after a further 37 incorrect codons.

(Weinzierl et al., 1987)

Deletion of 50bp at map position -31.5kb. very close to the site of the Doc element insertion in Ubx¹.

(Bender et al., 1983)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:16,733,352..16,733,383 [-]

Comment:

Mutation is a deletion

(Weinzierl et al., 1987)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | first instar larval stage

(Estacio-Gómez et al., 2013)

decreased cell number | first instar larval stage

(Estacio-Gómez et al., 2013)

visible | dominant

(de Navas et al., 2006)

visible | somatic clone

(de Navas et al., 2006)

Phenotype Manifest In

abdominal 1 ventral denticle belt | ectopic

(Hittinger et al., 2005)

abdominal segment 1 & haltere | ectopic

(Hittinger et al., 2005)

abdominal segment 1 & Keilin's organ | ectopic

(Hittinger et al., 2005)

abdominal segment 1 & metathoracic leg | ectopic

(Hittinger et al., 2005)

abdominal tergite 1 (with Ubx^ΔQA)

(Hittinger et al., 2005)

adult mesothoracic segment | anterior | ectopic (with Ubx^bx-8)

(Badenhorst et al., 2002)

adult mesothoracic segment | anterior | ectopic (with Ubx^bx-34e)

(Badenhorst et al., 2002)

adult metathoracic segment (with Ubx^bx-8)

(Badenhorst et al., 2002)

adult metathoracic segment (with Ubx^bx-34e)

(Badenhorst et al., 2002)

capitellum

(de Navas et al., 2006)

haltere

(Hittinger et al., 2005)

haltere (with Ubx^bx-8)

(Badenhorst et al., 2002)

haltere (with Ubx^bx-34e)

(Badenhorst et al., 2002)

haltere (with Ubx^ΔQA)

(Hittinger et al., 2005)

haltere | somatic clone

(de Navas et al., 2006)

haltere disc anterior compartment (with Ubx^bx-3)

(de Navas et al., 2006)

haltere disc posterior compartment (with Ubx^pbx-1)

(de Navas et al., 2006)

larval abdominal 1 A-subperineurial glial cell

(Prokop and Technau, 1994)

larval abdominal 1 B-subperineurial glial cell

(Prokop and Technau, 1994)

larval abdominal ganglion Leucokinin neuron | decreased number | first instar larval stage

(Estacio-Gómez et al., 2013)

larval abdominal neuromere | first instar larval stage

(Estacio-Gómez et al., 2013)

larval abdominal segment 1

(Hittinger et al., 2005)

larval metathoracic segment | ectopic

(Hittinger et al., 2005)

mesothoracic laterotergite & macrochaeta | ectopic (with Ubx^ΔQA)

(Hittinger et al., 2005)

mesothoracic laterotergite | ectopic (with Ubx^ΔQA)

(Hittinger et al., 2005)

metathoracic laterotergite (with Ubx^ΔQA)

(Hittinger et al., 2005)

metathoracic ventral denticle belt

(Hittinger et al., 2005)

trichome & mesothoracic leg (with Ubx^ΔQA)

(Hittinger et al., 2005)

trichome & metathoracic leg (with Ubx^ΔQA)

(Hittinger et al., 2005)

ventral denticle belt

(Weinzierl et al., 1987)

wing | ectopic (with Ubx^bx-8)

(Badenhorst et al., 2002)

wing | ectopic (with Ubx^bx-34e)

(Badenhorst et al., 2002)

wing | ectopic | somatic clone

(de Navas et al., 2006)

Detailed Description

Statement

Reference

Homozygous clones in the haltere discs of late third instar larvae are of similar size to their twin spots.

The capitellum of the haltere is enlarged compared to wild type in Ubx^6.28 heterozygotes.

The posterior compartment is increased in size in Ubx^6.28/Ubx^pbx-1 haltere discs.

The anterior compartment is increased in size in Ubx^6.28/Ubx^bx-3 haltere discs.

Homozygous clones in the haltere show transformation of haltere tissue to wing and the haltere is larger than that seen in wild-type flies.

(de Navas et al., 2006)

In Ubx^bx-3 Ubx^pbx-1 Ubx^6.28 L3 larvae, tracheal metamere 3 is transformed into tracheal metamere 2. Tracheal metamere 2 appears normal.

(Guha and Kornberg, 2005)

Ubx^6.28 heterozygotes exhibit halteres that are about twice the volume of wild-type and often have multiple ectopic bristles (2.59 bristles per haltere). Ubx^6.28 larvae form only partial two-bristle ectopic Keilin's Organs on the A1 segment, with only incomplete ectopic limb primordia formed in the A1 segment. Ubx^ΔQA/Ubx^6.28 larvae form ectopic A1 Keilin's Organs with up to two bristles. Ubx^6.28 heterozygotes only very rarely develop limited postnotal tissue, due to transformation of T3 towards a T2 identity. Ubx^6.28 heterozygous larvae exhibit mild transformations of the A1 denticle belts towards an intermediate T3/A1 identity. Ubx^6.28 homozygotes exhibit complete transformations of the A1 denticle belts to T2 identity. Ubx^ΔQA/Ubx^6.28 mutant T3 laterotergites transform towards a T2 thorax identity, as expressed by the number of ectopic bristles present. Ubx^ΔQA mutants adults have moderate transformations of the T3 laterotergite towards T2 identity, with up to three ectopic bristles per half-laterotergite. Ubx^ΔQA/Ubx^6.28 mutants exhibit extensive postnotal tissue, with up to a dozen ectopic bristles per half-laterotergite. Ubx^ΔQA/Ubx^6.28 adults exhibit severely reduced A1 tergites, which in extreme cases are nearly absent. In many Ubx^ΔQA/Ubx^6.28 adults, the combination of an extremely reduced A1 tergite and a partial transformation of the T3 laterotergite toward an intermediate T2/T3 postnotal identity create an indistinct boundary between the dorsal thorax and abdomen. In Ubx^ΔQA/Ubx^6.28 mutants, the area of naked cuticle which in wild-type is without non-sensory microtrichiae (trichomes) in the posterior of the T2 legs (T2p) is reduced, and in some cases, nearly eliminated, whereas the phenotype of Ubx^6.28 heterozygotes is closer to wild-type. In Ubx^ΔQA/Ubx^6.28 mutants, the area of naked cuticle which in wild-type is without non-sensory microtrichiae (trichomes) in the posterior of the T3 legs (T3p), exhibits expansive regions of trichomes formed distally and ventrally, while Ubx^6.28 heterozygous T3p exhibit only a few distal trichomes compared with the wild-type.

(Hittinger et al., 2005)

Ubx^6.28/Ubx^bx-34e and Ubx^6.28/Ubx^bx-8 mutant combinations cause a homeotic transformation of the third thoracic segment to the anterior second thoracic segment. The haltere (T3) is also transformed toward wing fate (t2), manifested by increases in size and the development of bristles.

(Badenhorst et al., 2002)

Embryos have no A- and B- cells in A1, where instead thorax-speciifc A/B-like glial cells could be detected, which do not derive from NB1-1.

(Prokop and Technau, 1994)

Expression of βTub60D in visceral mesoderm reduced, particularly in A1 and A2.

(Hinz et al., 1992)

Anterior constriction of the midgut has formed and is slightly more posterior as compared to wild type.

(Reuter and Scott, 1990)

Ubx^6.28 embryos have no dpp protein in the mesoderm of parasegments 6 or 7, a faint band can be seen at the posterior part of the domain directly adjacent to wg and abd-A expression.

(Reuter et al., 1990)

Homozygotes complete embryogenesis and occasionally survive as third instar larvae. They have the characteristic Ubx phenotype of transformed denticle belts and three pairs of anterior spiracles.

(Weinzierl et al., 1987)

The frequency of variegated bithorax transformations seen in Df(3R)red-P52 heterozygotes is not increased in flies also heterozygous for Ubx^6.28.

(Capdevila and Garcia-Bellido, 1981)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Ubx^6.28 has visible | dominant phenotype, enhanceable by Df(2L)tkv2/+

(de Navas et al., 2006)

Phenotype Manifest In

Enhanced by

Statement

Reference

Ubx^6.28 has capitellum phenotype, enhanceable by Df(2L)tkv2/+

(de Navas et al., 2006)

Ubx^6.28/Ubx^bx-8 has adult mesothoracic segment | anterior | ectopic phenotype, enhanceable by Df(3L)3643

(Badenhorst et al., 2002)

Ubx^6.28/Ubx^bx-8 has adult metathoracic segment phenotype, enhanceable by Df(3L)3643

(Badenhorst et al., 2002)

Ubx^6.28/Ubx^bx-34e has adult metathoracic segment phenotype, enhanceable by E(bx)^ry122

(Badenhorst et al., 2002)

Ubx^6.28/Ubx^bx-34e has haltere phenotype, enhanceable by E(bx)^ry122

(Badenhorst et al., 2002)

Ubx^6.28/Ubx^bx-34e has wing | ectopic phenotype, enhanceable by E(bx)^ry122

(Badenhorst et al., 2002)

Ubx^6.28/Ubx^bx-34e has adult mesothoracic segment | anterior | ectopic phenotype, enhanceable by E(bx)^ry122

(Badenhorst et al., 2002)

Ubx^6.28/Ubx^bx-8 has haltere phenotype, enhanceable by Df(3L)3643

(Badenhorst et al., 2002)

Ubx^6.28/Ubx^bx-8 has wing | ectopic phenotype, enhanceable by Df(3L)3643

(Badenhorst et al., 2002)

Ubx^6.28/Ubx^bx-8 has adult mesothoracic segment | anterior | ectopic phenotype, enhanceable by E(bx)^Nurf301-2

(Badenhorst et al., 2002)

Ubx^6.28/Ubx^bx-8 has adult metathoracic segment phenotype, enhanceable by E(bx)^Nurf301-2

(Badenhorst et al., 2002)

Ubx^6.28/Ubx^bx-34e has adult mesothoracic segment | anterior | ectopic phenotype, enhanceable by E(bx)^Nurf301-2

(Badenhorst et al., 2002)

Ubx^6.28/Ubx^bx-34e has adult metathoracic segment phenotype, enhanceable by E(bx)^Nurf301-2

(Badenhorst et al., 2002)

Ubx^6.28/Ubx^bx-34e has haltere phenotype, enhanceable by E(bx)^Nurf301-2

(Badenhorst et al., 2002)

Ubx^6.28/Ubx^bx-34e has wing | ectopic phenotype, enhanceable by E(bx)^Nurf301-2

(Badenhorst et al., 2002)

Suppressed by

Statement

Reference

Ubx^pbx-1/Ubx^6.28 has haltere disc posterior compartment phenotype, suppressible | partially by Scer\GAL4^en-e16E/tkv^UAS.cLa

(de Navas et al., 2006)

Ubx^bx-3/Ubx^6.28 has haltere disc anterior compartment phenotype, suppressible | partially by Scer\GAL4^ptc-559.1/tkv^UAS.cLa

(de Navas et al., 2006)

Other

Statement

Reference

Scr^hs.PZ, Ubx^6.28 has T1 beard & abdominal segment 1 phenotype

(Andrew et al., 1994)

Additional Comments

Genetic Interactions

Statement

Reference

The enlarged capitellum phenotype seen in Ubx^6.28 heterozygotes is enhanced by Df(2L)tkv2/+.

The increased size of the posterior compartment that is seen in the haltere discs of Ubx^6.28/Ubx^pbx-1 animals is partially suppressed by expression of tkv^Scer\UAS.cLa under the control of Scer\GAL4^en-e16E.

The increased size of the anterior compartment that is seen in the haltere discs of Ubx^6.28/Ubx^bx-3 animals is partially suppressed by expression of tkv^Scer\UAS.cLa under the control of Scer\GAL4^ptc-559.1.

(de Navas et al., 2006)

The addition of E(bx)^Nurf301-2, E(bx)^ry122 or Df(3L)3643 to Ubx^bx-34e/Ubx^6.28 animals enhances the anterior transformation seen in both the third thoracic segment and the haltere. The addition of E(bx)^Nurf301-2 or Df(3L)3643 to Ubx^bx-8/Ubx^6.28 animals enhances the anterior transformation seen in both the third thoracic segment and the haltere.

(Badenhorst et al., 2002)

Scr^hs.PZ expression in Ubx^6.28 heterozygotes or homozygotes causes T1 beard formation in abdominal segment 1.

(Andrew et al., 1994)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 2 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

X05723

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

CAA29194

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Ubx^6-28

(Kerridge and Morata, 1982, Capdevila and Garcia-Bellido, 1981)

Ubx^6.28

(Moris-Sanz et al., 2015, Estacio-Gómez et al., 2013, de Navas et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (22)

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