FlyBase Allele Report: Dmel\Nedd4[CA.UAS.Tag:MYC]

General Information

Symbol

Dmel\Nedd4^{CA.UAS.Tag:MYC}

Species

D. melanogaster

Name

FlyBase ID

FBal0141638

Feature type

allele

Associated gene

Dmel\Nedd4

Associated Insertion(s)

Carried in Construct

P{UAS-Nedd4.CA}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Myat et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-Nedd4.CA}

Cytology

Description

UAS regulatory sequences drive expression of a catalytically inactive form of Nedd4. The coding sequence is tagged with Tag:MYC.

(Myat et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Nedd4

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^dpp.blk1

(Wilkin et al., 2004)

Phenotype Manifest In

wing vein, with Scer\GAL4^dpp.blk1

(Wilkin et al., 2004)

Detailed Description

Statement

Reference

Expression of Nedd4^{CA.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^dpp.blk1 at 29^oC has little effect on the legs, but gaps in the wing veins are seen.

(Wilkin et al., 2004)

Over-expression of Nedd4^{CA.Scer\UAS.T:Hsap\MYC} in all neurons has no effect on axon outgrowth.

(Myat et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Nedd4^{CA.UAS.Tag:MYC}, Scer\GAL4^ptc-559.1 is an enhancer of visible phenotype of N^UAS.cMa, Scer\GAL4^ptc-559.1

(Wilkin et al., 2004)

NOT Enhancer of

Statement

Reference

Nedd4^{CA.UAS.Tag:MYC}, Scer\GAL4^elav.PLu is a non-enhancer of abnormal neuroanatomy | embryonic stage 16 phenotype of Scer\GAL4^elav.PLu, comm^UAS.Tag:MYC

(Keleman et al., 2005)

Suppressor of

Statement

Reference

Nedd4^{CA.UAS.Tag:MYC}, Scer\GAL4^dpp.blk1 is a suppressor | partially of visible phenotype of Scer\GAL4^dpp.blk1, Su(dx)^UAS.cCa

(Wilkin et al., 2004)

Nedd4^{CA.UAS.Tag:MYC}, Scer\GAL4^elav-C155 is a suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^elav-C155, comm^UAS.cWa

(Myat et al., 2002)

Phenotype Manifest In

Enhancer of

Statement

Reference

Nedd4^{CA.UAS.Tag:MYC}, Scer\GAL4^ptc-559.1 is an enhancer of wing margin | ectopic phenotype of N^UAS.cMa, Scer\GAL4^ptc-559.1

(Wilkin et al., 2004)

NOT Enhancer of

Statement

Reference

Nedd4^{CA.UAS.Tag:MYC}, Scer\GAL4^elav.PLu is a non-enhancer of larval longitudinal connective | embryonic stage 16 phenotype of Scer\GAL4^elav.PLu, comm^UAS.Tag:MYC

(Keleman et al., 2005)

Nedd4^{CA.UAS.Tag:MYC}, Scer\GAL4^elav.PLu is a non-enhancer of commissure | embryonic stage 16 phenotype of Scer\GAL4^elav.PLu, comm^UAS.Tag:MYC

(Keleman et al., 2005)

Suppressor of

Statement

Reference

Nedd4^{CA.UAS.Tag:MYC}, Scer\GAL4^dpp.blk1 is a suppressor of leg phenotype of Scer\GAL4^dpp.blk1, Su(dx)^UAS.cCa

(Wilkin et al., 2004)

Nedd4^{CA.UAS.Tag:MYC}, Scer\GAL4^dpp.blk1 is a suppressor | partially of wing vein L3 phenotype of Scer\GAL4^dpp.blk1, Su(dx)^UAS.cCa

(Wilkin et al., 2004)

Nedd4^{CA.UAS.Tag:MYC}, Scer\GAL4^dpp.blk1 is a suppressor of wing phenotype of Scer\GAL4^dpp.blk1, Su(dx)^UAS.cCa

(Wilkin et al., 2004)

Nedd4^{CA.UAS.Tag:MYC}, Scer\GAL4^elav-C155 is a suppressor of larval anterior commissure phenotype of Scer\GAL4^elav-C155, comm^UAS.cWa

(Myat et al., 2002)

Nedd4^{CA.UAS.Tag:MYC}, Scer\GAL4^elav-C155 is a suppressor of larval posterior commissure phenotype of Scer\GAL4^elav-C155, comm^UAS.cWa

(Myat et al., 2002)

Nedd4^{CA.UAS.Tag:MYC}, Scer\GAL4^elav-C155 is a suppressor of larval ventral nerve cord phenotype of Scer\GAL4^elav-C155, comm^UAS.cWa

(Myat et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Nedd4^{CA.Scer\UAS.T:Hsap\MYC} does not suppress the aberrant midline crossing phenotype seen when comm^{Scer\UAS.T:Hsap\MYC} is expressed under the control of Scer\GAL4^elav.PLu in stage 16 embryos.

(Keleman et al., 2005)

Co-expression of Nedd4^{CA.Scer\UAS.T:Hsap\MYC} rescues the leg, wing margin and wing vein phenotypes produced by expression of Su(dx)^Scer\UAS.cCa under the control of Scer\GAL4^dpp.blk1 and only a small amount of extra wing vein is still present in the rescued flies. The ectopic wing phenotype caused by expression of N^Scer\UAS.cMa under the control of Scer\GAL4^ptc-559.1 at 25^oC is enhanced by co-expression of Nedd4^{CA.Scer\UAS.T:Hsap\MYC}.

(Wilkin et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Nedd4^{CA.Scer\UAS.T:Hsap\MYC}

Nedd4^{CA.UAS.Tag:MYC}

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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