FlyBase Allele Report: Dmel\Nedd4[UAS.Tag:MYC]

General Information

Symbol

Dmel\Nedd4^UAS.Tag:MYC

Species

D. melanogaster

Name

FlyBase ID

FBal0141637

Feature type

allele

Associated gene

Dmel\Nedd4

Associated Insertion(s)

Carried in Construct

P{UAS-Nedd4.myc}

Also Known As

UAS-DNedd4, UAS-Nedd4

Key Links

Transgenic product class

wild_type

(Myat et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Myat et al., 2002)

Mutagen

in vitro construct

(Myat et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-Nedd4.myc}

Cytology

Description

UAS regulatory sequences drive expression of the Nedd4 coding region. The coding sequence is tagged with Tag:MYC.

(Myat et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Nedd4

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^ptc-559.1

(Wilkin et al., 2004)

Phenotype Manifest In

wing, with Scer\GAL4^ptc-559.1

(Wilkin et al., 2004)

Detailed Description

Statement

Reference

Expression of Nedd4^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PU at 28[o]C does not affect eye development.

Expression of Nedd4^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^elav.PU does not affect climbing ability or lifespan.

(Davies et al., 2014)

Expression of Nedd4^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^elav.PLu does not produce ectopic midline crossing.

(Keleman et al., 2005)

Expression of Nedd4^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ptc-559.1 results in notching of the wing.

(Wilkin et al., 2004)

Over-expression of Nedd4 in all neurons has no effect on axon outgrowth.

(Myat et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Nedd4^UAS.Tag:MYC, Scer\GAL4^elav-C155 is an enhancer of abnormal neuroanatomy phenotype of Scer\GAL4^elav-C155, comm^UAS.cWa

(Myat et al., 2002)

Scer\GAL4^elav-C155/Nedd4^UAS.Tag:MYC is an enhancer of abnormal neuroanatomy | dominant phenotype of robo1⁵, sli[+]/sli¹

(Myat et al., 2002)

NOT Enhancer of

Statement

Reference

Nedd4^UAS.Tag:MYC, Scer\GAL4^elav.PLu is a non-enhancer of abnormal neuroanatomy | embryonic stage 16 phenotype of Scer\GAL4^elav.PLu, comm^UAS.Tag:MYC

(Keleman et al., 2005)

Nedd4^UAS.Tag:MYC/Scer\GAL4^elav.PLu is a non-enhancer of abnormal neuroanatomy | embryonic stage 16 phenotype of robo1¹, sli²

(Keleman et al., 2005)

Scer\GAL4^insc-Mz1407/Nedd4^UAS.Tag:MYC is a non-enhancer of abnormal neuroanatomy | embryonic stage 16 phenotype of robo1¹, sli²

(Keleman et al., 2005)

Suppressor of

Statement

Reference

Nedd4^UAS.Tag:MYC, Scer\GAL4^elav.PU is a suppressor of abnormal locomotor behavior phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^elav.PU

(Davies et al., 2014)

Phenotype Manifest In

Enhancer of

Statement

Reference

Nedd4^UAS.Tag:MYC, Scer\GAL4^elav-C155 is an enhancer of larval anterior commissure phenotype of Scer\GAL4^elav-C155, comm^UAS.cWa

(Myat et al., 2002)

Nedd4^UAS.Tag:MYC, Scer\GAL4^elav-C155 is an enhancer of larval posterior commissure phenotype of Scer\GAL4^elav-C155, comm^UAS.cWa

(Myat et al., 2002)

Nedd4^UAS.Tag:MYC, Scer\GAL4^elav-C155 is an enhancer of larval ventral nerve cord phenotype of Scer\GAL4^elav-C155, comm^UAS.cWa

(Myat et al., 2002)

Scer\GAL4^elav-C155/Nedd4^UAS.Tag:MYC is an enhancer of larval anterior commissure phenotype of robo1⁵, sli[+]/sli¹

(Myat et al., 2002)

Scer\GAL4^elav-C155/Nedd4^UAS.Tag:MYC is an enhancer of larval posterior commissure phenotype of robo1⁵, sli[+]/sli¹

(Myat et al., 2002)

Scer\GAL4^elav-C155/Nedd4^UAS.Tag:MYC is an enhancer of larval ventral nerve cord phenotype of robo1⁵, sli[+]/sli¹

(Myat et al., 2002)

NOT Enhancer of

Statement

Reference

Nedd4^UAS.Tag:MYC/Scer\GAL4^elav.PLu is a non-enhancer of larval longitudinal connective | embryonic stage 16 phenotype of robo1¹, sli²

(Keleman et al., 2005)

Nedd4^UAS.Tag:MYC/Scer\GAL4^elav.PLu is a non-enhancer of commissure | embryonic stage 16 phenotype of robo1¹, sli²

(Keleman et al., 2005)

Scer\GAL4^insc-Mz1407/Nedd4^UAS.Tag:MYC is a non-enhancer of larval longitudinal connective | embryonic stage 16 phenotype of robo1¹, sli²

(Keleman et al., 2005)

Scer\GAL4^insc-Mz1407/Nedd4^UAS.Tag:MYC is a non-enhancer of commissure | embryonic stage 16 phenotype of robo1¹, sli²

(Keleman et al., 2005)

Nedd4^UAS.Tag:MYC, Scer\GAL4^elav.PLu is a non-enhancer of larval longitudinal connective | embryonic stage 16 phenotype of Scer\GAL4^elav.PLu, comm^UAS.Tag:MYC

(Keleman et al., 2005)

Nedd4^UAS.Tag:MYC, Scer\GAL4^elav.PLu is a non-enhancer of commissure | embryonic stage 16 phenotype of Scer\GAL4^elav.PLu, comm^UAS.Tag:MYC

(Keleman et al., 2005)

Suppressor of

Statement

Reference

Nedd4^UAS.Tag:MYC, Scer\GAL4^GMR.PU is a suppressor of eye | heat sensitive phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^GMR.PU

(Davies et al., 2014)

Nedd4^UAS.Tag:MYC/Scer\GAL4^GMR.PU is a suppressor of dopaminergic neuron phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^elav.PU

(Davies et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Nedd4^{Scer\UAS.T:Hsap\MYC} does not enhance the aberrant midline crossing phenotype seen when comm^{Scer\UAS.T:Hsap\MYC} is expressed under the control of Scer\GAL4^elav.PLu in stage 16 embryos.

Expression of Nedd4^{Scer\UAS.T:Hsap\MYC} under the control of either Scer\GAL4^elav.PLu or Scer\GAL4¹⁴⁰⁷ does not enhance the midline crossing phenotype seen in transheterozygous sli² robo¹ stage 16 embryos.

(Keleman et al., 2005)

In sli¹/+,robo⁵/+ double heterozygote embryos the occasional axon crosses the midline. This phenotype is dramatically enhanced by Nedd4^{Scer\UAS.T:Hsap\MYC}.

(Myat et al., 2002)

Xenogenetic Interactions

Statement

Reference

Co-expression of Nedd4^{Scer\UAS.T:Hsap\MYC} suppresses the rough eye phenotype induced by expression of Hsap\SNCA^Scer\UAS.cFa in the developing eye under the control of Scer\GAL4^GMR.PU.

Co-expression of Nedd4^{Scer\UAS.T:Hsap\MYC} prevents the climbing deficit associated with neuronal Hsap\SNCA^Scer\UAS.cFa expression (both constructs under the control of Scer\GAL4^elav.PU.

Co-expression of Nedd4^{Scer\UAS.T:Hsap\MYC} prevents Hsap\SNCA^Scer\UAS.cFa-induced dopaminergic cell loss.

(Davies et al., 2014)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Nedd4^{Scer\UAS.T:Hsap\MYC}

Nedd4^UAS.Tag:MYC

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

Open Close