Expression in the midgut is found in a distinct group of Dh31-expressing enteroendocrine cells located at the junction region of the anterior midgut and the acid-secreting portion of the larval midgut. This junction region occurs at a conspicuous and characteristic U-shaped bend in the gut. These are referred to as the "midgut junction Dh31-expressing cells".

In the male reproductive tract, ScerGAL4^MJ33a drives expression in testis pigment cells and cyst cells. In embryos, ScerGAL4^MJ33a drives expression in the amnioserosa at stage 14. At larval stages, ScerGAL4^MJ33a drives expression in the wing disc in a weak pattern centered on the A/P boundary, in the eye disc anterior to the morphogenetic furrow, ubiquitously in leg discs, in the gut, and in the salivary glands.