Expression in the midgut is found in a distinct group of Dh31-expressing enteroendocrine cells located at the junction region of the anterior midgut and the acid-secreting portion of the larval midgut. This junction region occurs at a conspicuous and characteristic U-shaped bend in the gut. These are referred to as the "midgut junction Dh31-expressing cells".

Expression in the midgut is found in a distinct group of Dh31-expressing enteroendocrine cells located at the junction region of the anterior midgut and the acid-secreting portion of the larval midgut. This junction region occurs at a conspicuous and characteristic U-shaped bend in the gut. These are referred to as the "midgut junction Dh31-expressing cells". Expression is also observed in the proventriculus.

ScerGAL4^cb20 drives expression in the embyonic longitudinal muscle, in the larval central nervous system including the brain and mushroom body, and in the adult testis. During oogenesis, ScerGAL4^cb20 drives expression in terminal filaments and/or female germline stem cells, in dorsal appendage forming follicle cells, and in nurse cells near the oocyte at stages S1-10.