FB2024_03 , released June 25, 2024
Allele: Dmel\Hsp22EP3247
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General Information
Symbol
Dmel\Hsp22EP3247
Species
D. melanogaster
Name
FlyBase ID
FBal0138481
Feature type
allele
Associated gene
Dmel\Hsp22
Associated Insertion(s)
P{EP}CG4456EP3247
Carried in Construct
Also Known As
EP(3)3247
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
P-element activity
(Bidet et al., 2003, Pena-Rangel et al., 2002, Gene Disruption Project members, 2001-)
Progenitor genotype
Associated Insertion(s)
P{EP}CG4456EP3247
Cytology
Description

P{EP} insertion 643bp upstream of the Hsp22 translation initiation codon. The P{EP} element is oriented such that expression from the element should result in overexpression of Hsp22.

(Bidet et al., 2003)

The P{EP}Hsp67BbEP3247 insertion is within a dicistronic transcript which encodes two open reading frames, represented by Hsp22 and Hsp67Bb. The insert is oriented in the same direction as the direction of transcription and is downstream of the Hsp67Bb open reading frame and upstream of the Hsp22 open reading frame.

(FlyBase Genome Annotators, 2002-2003)
Allele components
Component
Use(s)
Inserted element
P{EP}
Regulatory region(s)
UAS
Mutations Mapped to the Genome
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Animals expressing Hsp22EP3247 under the control of Scer\GAL4Act5C.PI show a 29.2% increase in mean lifespan compared to controls, and the maximum lifespan of the mutant flies is approximately 115 days compared to approximately 95 days in controls.

      (Kim et al., 2010)

      Expression of Hsp22EP3247 under the control of Scer\GAL4Act is able to extend the mean life span under hyperoxic conditions by 15%.

      Expression of Hsp22EP3247 under the control of Scer\GAL4ple.PF rescues the degeneration of dopaminergic neurons that is seen in flies under hyperoxia.

      (Gruenewald et al., 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Statement
      Reference

      Hsp22EP3247/Scer\GAL4pnr-MD237 is a suppressor of visible phenotype of pnrMD237

      (Pena-Rangel et al., 2002)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      Selected as: a P{EP} insertion line that modifies the pnrMD237/+ phenotype when expressed using Scer\GAL4pnr-MD237.

      (Pena-Rangel et al., 2002)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Hsp22EP3247
      Name Synonyms
      Secondary FlyBase IDs
        References (6)