FlyBase Allele Report: Dmel\Akt[UAS.cRa]

General Information

Symbol

Dmel\Akt^UAS.cRa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Rintelen

FlyBase ID

FBal0136146

Feature type

allele

Associated gene

Dmel\Akt

Associated Insertion(s)

Carried in Construct

P{UAS-Akt.R}

Also Known As

UAS-PKB

Key Links

Transgenic product class

wild_type

(Rintelen et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Rintelen et al., 2001)

Mutagen

in vitro construct

(Rintelen et al., 2001)

Progenitor genotype

Carried in construct

P{UAS-Akt.R}

Cytology

Description

UAS regulatory sequences drive expression of Akt1.

(Rintelen et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Akt

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^Toll-6-D42

(Martin-Pena et al., 2006)

visible, with Scer\GAL4^GMR.PF

(Radimerski et al., 2002)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Radimerski et al., 2002)

synapse | increased number, with Scer\GAL4^Toll-6-D42

(Martin-Pena et al., 2006)

Detailed Description

Statement

Reference

Expression of Akt1^Scer\UAS.cRa in the projection neurons, under the control of Scer\GAL4^GH146 or Scer\GAL4^{exba-krasavietz}, does not affect central or cross adaptation to EB or IAA odours.

Expression of Akt1^Scer\UAS.cRa in both the eLNs and iLNs, under the control of both Scer\GAL4^GH298 and Scer\GAL4^{exba-krasavietz} results in normal adaption to EB or IAA, indicating that olfactory central adaption depends on the eLN/iLN activity ratio.

(Acebes et al., 2012)

Larval expression of Akt1^Scer\UAS.cRa under the control of Scer\GAL4^D42 induced a two-fold increase in synapse number compared to wild-type.

Expression of Akt1^Scer\UAS.cRa under the control of Scer\GAL4⁷⁹⁶ increases the synaptic domain, with no significant change in cell number.

(Martin-Pena et al., 2006)

Expression of Akt1^Scer\UAS.cRa under the control of Scer\GAL4^ap-md544 has no effect on the wing. Animals expressing Akt1^Scer\UAS.cRa under the control of Scer\GAL4^GMR.PF have a slightly larger eye than normal.

(Radimerski et al., 2002)

Expression of Akt1^Scer\UAS.cRa under the control of Scer\GAL4^GMR.PF has little effect on the size or structure of the eye. Expression of Akt1^Scer\UAS.cRa under the control of Scer\GAL4^GMR.PB in clones of cells in the eye does not increase cell size.

(Rintelen et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Akt^UAS.cRa, Pdk1^UAS.cRa, Scer\GAL4^GMR.PF has increased body size phenotype, enhanceable by scyl³¹/scyl¹¹³

(Reiling and Hafen, 2004)

Suppressed by

Statement

Reference

Akt^UAS.cRa, Pdk1^EP837, Scer\GAL4^GMR.PF has increased body size phenotype, suppressible by Lk6^EP30.18

(Reiling et al., 2005)

Akt^UAS.cRa, Pdk1^EP837, Scer\GAL4^GMR.PF has increased body size phenotype, suppressible by chrb^EP1035

(Reiling and Hafen, 2004)

Akt^UAS.cRa, Pdk1^EP837, Scer\GAL4^GMR.PF has increased body size phenotype, suppressible by scyl^EP22.1

(Reiling and Hafen, 2004)

Enhancer of

Statement

Reference

Akt^UAS.cRa, Scer\GAL4^Bx-MS1096 is an enhancer of visible phenotype of Pi3K92E^UAS.Tag:MYC, Scer\GAL4^Bx-MS1096

(Radimerski et al., 2002)

Akt^UAS.cRa, Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF

(Radimerski et al., 2002)

Suppressor of

Statement

Reference

Akt^UAS.cRa, Scer\GAL4^ap-md544 is a suppressor | partially of visible phenotype of S6k^UAS.cMa, Scer\GAL4^ap-md544

(Radimerski et al., 2002)

Other

Statement

Reference

Akt^UAS.cRa, Pdk1^EP837, Scer\GAL4^GMR.PF has increased body size phenotype

(Reiling et al., 2005, Reiling and Hafen, 2004)

Akt^UAS.cRa, Pdk1^UAS.cRa, Scer\GAL4^GMR.PF has increased body size phenotype

(Reiling and Hafen, 2004)

Akt^UAS.cRa, Pdk1^EP837, Scer\GAL4^GMR.PF has increased cell size | somatic clone | cell autonomous phenotype

(Rintelen et al., 2001)

Phenotype Manifest In

Enhanced by

Statement

Reference

Akt^UAS.cRa, Pdk1^UAS.cRa, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by scyl³¹/scyl¹¹³

(Reiling and Hafen, 2004)

Suppressed by

Statement

Reference

Akt^UAS.cRa, Pdk1^EP837, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Lk6^EP30.18

(Reiling et al., 2005)

Akt^UAS.cRa, Pdk1^EP837, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Lk6^EP30.18, Scer\GAL4^GMR.PF

(Reiling et al., 2005)

Akt^UAS.cRa, Pdk1^EP837, Scer\GAL4^GMR.PF has eye phenotype, suppressible by chrb^EP1035

(Reiling and Hafen, 2004)

Akt^UAS.cRa, Pdk1^EP837, Scer\GAL4^GMR.PF has eye phenotype, suppressible by scyl^EP22.1

(Reiling and Hafen, 2004)

Enhancer of

Statement

Reference

Akt^UAS.cRa, Scer\GAL4^Bx-MS1096 is an enhancer of wing phenotype of Pi3K92E^UAS.Tag:MYC, Scer\GAL4^Bx-MS1096

(Radimerski et al., 2002)

Akt^UAS.cRa, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF

(Radimerski et al., 2002)

Akt^UAS.cRa, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Pdk1^EP837, Scer\GAL4^GMR.PF

(Rintelen et al., 2001)

Suppressor of

Statement

Reference

Akt^UAS.cRa, Scer\GAL4^ap-md544 is a suppressor | partially of wing phenotype of S6k^UAS.cMa, Scer\GAL4^ap-md544

(Radimerski et al., 2002)

Other

Statement

Reference

Akt^UAS.cRa, Pdk1^EP837, Scer\GAL4^GMR.PF has eye phenotype

(Reiling et al., 2005, Reiling and Hafen, 2004)

Akt^UAS.cRa, Pdk1^UAS.cRa, Scer\GAL4^GMR.PF has eye phenotype

(Reiling and Hafen, 2004)

Akt^UAS.cRa, Pdk1^EP837, Scer\GAL4^GMR.PF has eye | somatic clone | cell autonomous phenotype

(Rintelen et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

Animals expressing both Akt1^Scer\UAS.cRa and Pk61C^EP837 under the control of Scer\GAL4^GMR.PF have a large-eye phenotype. Expression of Lk6^EP30.18 in this background strongly suppresses this phenotype.

(Reiling et al., 2005)

Flies co-expressing Akt1^Scer\UAS.cRa and Pk61C^EP837 under the control of Scer\GAL4^GMR.PF have a large eye phenotype. Flies co-expressing Akt1^Scer\UAS.cRa and Pk61C^Scer\UAS.cRa under the control of Scer\GAL4^GMR.PF have a large eye phenotype. The large eye phenotype caused by co-expression of Akt1^Scer\UAS.cRa and Pk61C^EP837 under the control of Scer\GAL4^GMR.PF is suppressed by co-expression of scyl^EP22.1. The large eye phenotype caused by co-expression of Akt1^Scer\UAS.cRa and Pk61C^EP837 under the control of Scer\GAL4^GMR.PF is suppressed by co-expression of chrb^EP1035. The large eye phenotype caused by co-expression of Akt1^Scer\UAS.cRa and Pk61C^EP837 under the control of Scer\GAL4^GMR.PF is suppressed nearly to wild type by simultaneous co-expression of both scyl^EP22.1 and chrb^EP1035. The large eye phenotype caused by co-expression of Akt1^Scer\UAS.cRa and Pk61C^Scer\UAS.cRa under the control of Scer\GAL4^GMR.PF is enhanced if the flies also carry scyl³¹/scyl¹¹³.

(Reiling and Hafen, 2004)

Co-expression of Akt1^Scer\UAS.cRa slightly suppresses the bent wing phenotype caused by expression of S6k^Scer\UAS.cMa under the control of Scer\GAL4^ap-md544. Co-expression of Akt1^Scer\UAS.cRa results in a clear increase the size of the eye and individual ommatidia in animals expressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PF. Co-expression of Akt1^Scer\UAS.cRa in animals expressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Bx-MS1096 causes the wing to bend strongly downwards.

(Radimerski et al., 2002)

Coexpression of Akt1^Scer\UAS.cRa and Pk61C^EP837 under the control of Scer\GAL4^GMR.PF causes a substantial increase in eye size. Coexpression of Akt1^Scer\UAS.cRa and Pk61C^EP837 under the control of Scer\GAL4^GMR.PB in clones of cells in the eye results in a cell autonomous increase in cell size.

(Rintelen et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Akt1^Scer\UAS.cRa

Akt1^UAS.cRa

Akt^UAS.cRa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Rintelen

(Rintelen et al., 2001)

Secondary FlyBase IDs

References (6)

Report Sections

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