FB2024_03 , released June 25, 2024
Allele: Dmel\spn-F2
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General Information
Symbol
Dmel\spn-F2
Species
D. melanogaster
Name
FlyBase ID
FBal0129430
Feature type
allele
Associated gene
Dmel\spn-F
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Abdu et al., 2006)
Mutagen
Delta2-3 transposase
(Deak et al., 1997)
Progenitor genotype
Cytology
Description

spn-F2 mutants are caused by a 67-nucleotide deletion starting from nucleotide 726.

(Abdu et al., 2006)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
3R:31,255,968..31,256,034 [+]
Comment:

A 67 nucleotide deletion starting at base 726 (where +1 is the A of the initiator Met).

(Abdu et al., 2006)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      spn-F2/spn-F2 or spn-F2/Df(3R)tll-e pupae have dendrite pruning defects (16-18h after puparium formation). Dendrites develop normally in sensory neurons (C4da) of spn-F2/spn-F2 larvae.

      (Lin et al., 2015)

      Both hemizygous and homozygous spn-F2 individuals are female sterile and show bristle defects.

      spn-F2 and spn-F2/Df(3R)tll-e ventralised eggshells exhibit a variety of phenotypes. The majority of these eggs (approximately 84-88%) do not have appendages, with smaller percentages exhibiting a single appendage or fusing of the appendages at the base.

      (Abdu et al., 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT suppressed by
      Statement
      Reference

      spn-F2 has abnormal neuroanatomy | pupal stage phenotype, non-suppressible by Scer\GAL4ppk.PG/IKKεGD5424

      (Lin et al., 2015)
      Suppressor of
      Phenotype Manifest In
      NOT suppressed by
      Statement
      Reference

      spn-F2 has dendrite | pupal stage phenotype, non-suppressible by Scer\GAL4ppk.PG/IKKεGD5424

      (Lin et al., 2015)
      Suppressor of
      Statement
      Reference

      spn-F2/spn-F2 is a suppressor | partially of dendrite | larval stage phenotype of IKKεUAS.cKa, Scer\GAL4ppk.PG

      (Lin et al., 2015)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Dendrite pruning defects in spn-F2/spn-F2 pupae are not suppressed when IKKεGD5424 is driven by Scer\GAL4ppk.PG.

      spn-F2/spn-F2 partially suppresses the dendrite severing and apoptosis seen in larvae with IKKεScer\UAS.cKa overexpressed by Scer\GAL4ppk.PG in combination with Scer\GAL80ts.αTub84B at non-permissive (29[o]C) temperatures.

      (Lin et al., 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      spn-F2 is rescued by spn-FUAS.EGFP/Scer\GAL4ppk.PG

      (Lin et al., 2015)

      spn-F2 is rescued by spn-F8D.UAS.EGFP/Scer\GAL4ppk.PG

      (Lin et al., 2015)

      spn-F2 is rescued by Scer\GAL4VP16.nanos.UTR/spn-FUAS.cAa

      (Abdu et al., 2006)

      spn-F2 is rescued by spn-FUAS.cAa/Scer\GAL4Act5C.PI

      (Abdu et al., 2006)
      Partially rescued by

      spn-F2 is partially rescued by Scer\GAL4ppk.PG/spn-F8A.UAS.EGFP

      (Lin et al., 2015)

      spn-F2 is partially rescued by spn-FΔSCD.UAS.EGFP/Scer\GAL4ppk.PG

      (Lin et al., 2015)
      Not rescued by

      spn-F2 is not rescued by Scer\GAL4ppk.PG/spn-FΔCC3.UAS.EGFP

      (Lin et al., 2015)
      Comments

      spn-FScer\UAS.T:Avic\GFP-EGFP or spn-F8D.Scer\UAS.T:Avic\GFP-EGFP expression driven by Scer\GAL4ppk.PG rescues impaired dendrite pruning in spn-F2/spn-F2 pupae (16-18h after puparium formation).

      spn-F8A.Scer\UAS.T:Avic\GFP-EGFP or spn-FΔSCD.Scer\UAS.T:Avic\GFP-EGFP expression driven by Scer\GAL4ppk.PG partially rescues impaired dendrite pruning in spn-F2/spn-F2 pupae (16h after puparium formation).

      spn-FΔCC3.Scer\UAS.T:Avic\GFP-EGFP expression driven by Scer\GAL4ppk.PG fails to rescue dendrite pruning defects in spn-F2/spn-F2 pupae (16h after puparium formation).

      (Lin et al., 2015)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      Separable from: P{lacW}S113003a.

      (Deak et al., 1997)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      1130/03
      (Deak et al., 1997)
      Spn-F2
      (Lu et al., 2020)
      l(3)S113003bS113003b
      spn-F2
      (Lin et al., 2020, Lin et al., 2015, Dubin-Bar et al., 2008, Abdu et al., 2006)
      Name Synonyms
      Secondary FlyBase IDs
        References (6)